pGL4.26
pGL4.26
编号 | 载体名称 |
北京华越洋生物VECT6094 | pGL4.26 |
pGL4.26载体基本信息
载体名称: | pGL4.26 、pGL4.26[luc2/minP/Hygro] |
质粒类型: | 哺乳细胞表达；信号通路报告载体 |
高拷贝/低拷贝: | -- |
启动子: | 弱启动子 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 5882 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素Ampicillin |
筛选标记: | 潮霉素Hygromycin |
备注: | pGL4.26载体含有荧光素酶报告基因luc2和弱启动子，用于研究细胞内信号通路中转录应答元件的活性。 |
稳定性: | -- |
组成型: | 组成型 |
病毒/非病毒: | 非病毒 |
pGL4.26载体质粒图谱和多克隆位点信息
pGL4.26载体简介
The pGL4.26[luc2/minP/Hygro] Vector(a–e) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and the luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.
pGL4.26载体序列
ORIGIN
1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
61 GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG ACTTCCAGCT TGGCAATCCG
121 GTACTGTTGG TAAAGCCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT
181 TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG
241 CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG
301 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA
361 CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG
421 GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC
481 TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC
541 AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA
601 GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC
661 CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC
721 TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA
781 CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG
841 ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG
901 GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT
961 TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT
1021 TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA
1081 GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC
1141 CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG
1201 AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG
1261 TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG
1321 GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA
1381 AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA
1441 TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC
1501 TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG
1561 ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA
1621 CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG
1681 GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA
1741 AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATAATTCT
1801 AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
1861 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
1921 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
1981 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA
2041 AATGTGGTAA AATCGATAAG GATCCGTTTG CGTATTGGGC GCTCTTCCGC TGATCTGCGC
2101 AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGCTACCT TCTGAGGCGG
2161 AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC
2221 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC
2281 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT
2341 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC
2401 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT
2461 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCGATTC
2521 TTCTGACACT AGCGCCACCA TGAAGAAGCC CGAACTCACC GCTACCAGCG TTGAAAAATT
2581 TCTCATCGAG AAGTTCGACA GTGTGAGCGA CCTGATGCAG TTGTCGGAGG GCGAAGAGAG
2641 CCGAGCCTTC AGCTTCGATG TCGGCGGACG CGGCTATGTA CTGCGGGTGA ATAGCTGCGC
2701 TGATGGCTTC TACAAAGACC GCTACGTGTA CCGCCACTTC GCCAGCGCTG CACTACCCAT
2761 CCCCGAAGTG TTGGACATCG GCGAGTTCAG CGAGAGCCTG ACATACTGCA TCAGTAGACG
2821 CGCCCAAGGC GTTACTCTCC AAGACCTCCC CGAAACAGAG CTGCCTGCTG TGTTACAGCC
2881 TGTCGCCGAA GCTATGGATG CTATTGCCGC CGCCGACCTC AGTCAAACCA GCGGCTTCGG
2941 CCCATTCGGG CCCCAAGGCA TCGGCCAGTA CACAACCTGG CGGGATTTCA TTTGCGCCAT
3001 TGCTGATCCC CATGTCTACC ACTGGCAGAC CGTGATGGAC GACACCGTGT CCGCCAGCGT
3061 AGCTCAAGCC CTGGACGAAC TGATGCTGTG GGCCGAAGAC TGTCCCGAGG TGCGCCACCT
3121 CGTCCATGCC GACTTCGGCA GCAACAACGT CCTGACCGAC AACGGCCGCA TCACCGCCGT
3181 AATCGACTGG TCCGAAGCTA TGTTCGGGGA CAGTCAGTAC GAGGTGGCCA ACATCTTCTT
3241 CTGGCGGCCC TGGCTGGCTT GCATGGAGCA GCAGACTCGC TACTTCGAGC GCCGGCATCC
3301 CGAGCTGGCC GGCAGCCCTC GTCTGCGAGC CTACATGCTG CGCATCGGCC TGGATCAGCT
3361 CTACCAGAGC CTCGTGGACG GCAACTTCGA CGATGCTGCC TGGGCTCAAG GCCGCTGCGA
3421 TGCCATCGTC CGCAGCGGGG CCGGCACCGT CGGTCGCACA CAAATCGCTC GCCGGAGCGC
3481 AGCCGTATGG ACCGACGGCT GCGTCGAGGT GCTGGCCGAC AGCGGCAACC GCCGGCCCAG
3541 TACACGACCG CGCGCTAAGG AGGTAGGTCG AGTTTAAACT CTAGAACCGG TCATGGCCGC
3601 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGT TCGAACTAGA
3661 TGCTGTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG
3721 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG
3781 GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
3841 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
3901 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
3961 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
4021 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
4081 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
4141 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
4201 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
4261 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
4321 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
4381 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
4441 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
4501 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
4561 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
4621 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
4681 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCGGCCG
4741 CAAATGCTAA ACCACTGCAG TGGTTACCAG TGCTTGATCA GTGAGGCACC GATCTCAGCG
4801 ATCTGCCTAT TTCGTTCGTC CATAGTGGCC TGACTCCCCG TCGTGTAGAT CACTACGATT
4861 CGTGAGGGCT TACCATCAGG CCCCAGCGCA GCAATGATGC CGCGAGAGCC GCGTTCACCG
4921 GCCCCCGATT TGTCAGCAAT GAACCAGCCA GCAGGGAGGG CCGAGCGAAG AAGTGGTCCT
4981 GCTACTTTGT CCGCCTCCAT CCAGTCTATG AGCTGCTGTC GTGATGCTAG AGTAAGAAGT
5041 TCGCCAGTGA GTAGTTTCCG AAGAGTTGTG GCCATTGCTA CTGGCATCGT GGTATCACGC
5101 TCGTCGTTCG GTATGGCTTC GTTCAACTCT GGTTCCCAGC GGTCAAGCCG GGTCACATGA
5161 TCACCCATAT TATGAAGAAA TGCAGTCAGC TCCTTAGGGC CTCCGATCGT TGTCAGAAGT
5221 AAGTTGGCCG CGGTGTTGTC GCTCATGGTA ATGGCAGCAC TACACAATTC TCTTACCGTC
5281 ATGCCATCCG TAAGATGCTT TTCCGTGACC GGCGAGTACT CAACCAAGTC GTTTTGTGAG
5341 TAGTGTATAC GGCGACCAAG CTGCTCTTGC CCGGCGTCTA TACGGGACAA CACCGCGCCA
5401 CATAGCAGTA CTTTGAAAGT GCTCATCATC GGGAATCGTT CTTCGGGGCG GAAAGACTCA
5461 AGGATCTTGC CGCTATTGAG ATCCAGTTCG ATATAGCCCA CTCTTGCACC CAGTTGATCT
5521 TCAGCATCTT TTACTTTCAC CAGCGTTTCG GGGTGTGCAA AAACAGGCAA GCAAAATGCC
5581 GCAAAGAAGG GAATGAGTGC GACACGAAAA TGTTGGATGC TCATACTCGT CCTTTTTCAA
5641 TATTATTGAA GCATTTATCA GGGTTACTAG TACGTCTCTC AAGGATAAGT AAGTAATATT
5701 AAGGTACGGG AGGTATTGGA CAGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG
5761 TGTTGGTTTT TTGTGTGAAT CGATAGTACT AACATACGCT CTCCATCAAA ACAAAACGAA
5821 ACAAAACAAA CTAGCAAAAT AGGCTGTCCC CAGTGCAAGT GCAGGTGCCA GAACATTTCT
5881 CT
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其他哺乳动物表达载体：
