pTRE2
pTRE2
编号 | 载体名称 |
北京华越洋生物VECT6091 | pTRE2 |
pTRE2载体基本信息
载体名称: | pTRE2 |
质粒类型: | 哺乳细胞表达；四环素调控载体 |
高拷贝/低拷贝: | -- |
启动子: | Tet-responsive |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 3757bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
备注: | -- |
稳定性: | 瞬表达 |
组成型: | 诱导型 |
病毒/非病毒: | 非病毒 |
pTRE2载体质粒图谱和多克隆位点信息
pTRE2载体序列
ORIGIN
1 CTCGAGTTTA CCACTCCCTA TCAGTGATAG AGAAAAGTGA AAGTCGAGTT TACCACTCCC
61 TATCAGTGAT AGAGAAAAGT GAAAGTCGAG TTTACCACTC CCTATCAGTG ATAGAGAAAA
121 GTGAAAGTCG AGTTTACCAC TCCCTATCAG TGATAGAGAA AAGTGAAAGT CGAGTTTACC
181 ACTCCCTATC AGTGATAGAG AAAAGTGAAA GTCGAGTTTA CCACTCCCTA TCAGTGATAG
241 AGAAAAGTGA AAGTCGAGTT TACCACTCCC TATCAGTGAT AGAGAAAAGT GAAAGTCGAG
301 CTCGGTACCC GGGTCGAGTA GGCGTGTACG GTGGGAGGCC TATATAAGCA GAGCTCGTTT
361 AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA
421 CCGGGACCGA TCCAGCCTCC GCGGCCCCGA ATTCGAGCTC GGTACCCGGG GATCCTCTAG
481 TCAGCTGACG CGTGCTAGCG CGGCCGCATC GATAAGCTTG TCGACGATAT CTCTAGAGCT
541 GAGAACTTCA GGGTGAGTTT GGGGACCCTT GATTGTTCTT TCTTTTTCGC TATTGTAAAA
601 TTCATGTTAT ATGGAGGGGG CAAAGTTTTC AGGGTGTTGT TTAGAATGGG AAGATGTCCC
661 TTGTATCACC ATGGACCCTC ATGATAATTT TGTTTCTTTC ACTTTCTACT CTGTTGACAA
721 CCATTGTCTC CTCTTATTTT CTTTTCATTT TCTGTAACTT TTTTCGTTAA ACTTTAGCTT
781 GCATTTGTAA CGAATTTTTA AATTCACTTT CGTTTATTTG TCAGATTGTA AGTACTTTCT
841 CTAATCACTT TTTTTTCAAG GCAATCAGGG TAATTATATT GTACTTCAGC ACAGTTTTAG
901 AGAACAATTG TTATAATTAA ATGATAAGGT AGAATATTTC TGCATATAAA TTCTGGCTGG
961 CGTGGAAATA TTCTTATTGG TAGAAACAAC TACATCCTGG TAATCATCCT GCCTTTCTCT
1021 TTATGGTTAC AATGATATAC ACTGTTTGAG ATGAGGATAA AATACTCTGA GTCCAAACCG
1081 GGCCCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
1141 CTGGTTGTTG TGCTGTCTCA TCATTTTGGC AAAGAATTCA CTCCTCAGGT GCAGGCTGCC
1201 TATCAGAAGG TGGTGGCTGG TGTGGCCAAT GCCCTGGCTC ACAAATACCA CTGAGATCTT
1261 TTTCCCTCTG CCAAAAATTA TGGGGACATC ATGAAGCCCC TTGAGCATCT GACTTCTGGG
1321 TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTGGGA ATTTTTTGTG TCTCTCACTC
1381 GGAAGGACAT ATGGGAGGGC AAATCATTTA AAACATCAGA ATGAGTATTT GGTTTAGAGT
1441 TTGGCAACAT ATGCCATATG CTGGCTGCCA TGAACAAAGG TGGCTATAAA GAGGTCATCA
1501 GTATATGAAA CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG
1561 TTAGATTTTT TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC
1621 CTTACATGTT TTACTAGCCA GATTTTTCCT CCTCTCCTGA CTACTCCCAG TCATAGCTGT
1681 CCCTCTTCTC TTATGAACTC GACTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG
1741 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
1801 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
1861 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
1921 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
1981 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
2041 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
2101 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC
2161 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
2221 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
2281 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
2341 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
2401 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
2461 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
2521 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
2581 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
2641 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
2701 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
2761 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
2821 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
2881 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
2941 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
3001 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
3061 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
3121 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
3181 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
3241 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
3301 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
3361 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
3421 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
3481 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
3541 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
3601 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
3661 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT
3721 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGT
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其他哺乳动物表达载体：
