pTK-hyg
pTK-hyg
编号 | 载体名称 |
北京华越洋生物VECT6089 | pTK-hyg |
pTK-hyg载体基本信息
载体名称: | pTK-hyg |
质粒类型: | 哺乳细胞表达；四环素调控载体 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 5.1kb |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | Amp |
筛选标记: | Hyg |
备注: | -- |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
pTK-hyg载体质粒图谱和多克隆位点信息
pTK-hyg载体序列
CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCCAGACATG ATAAGATACA TTGATGAGTT
TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC
TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT
TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT
CTACAAATGT GGTATGGCTG ATTATGATCC CCAGGAAGCT CCTCTGTGTC CTCATAAACC
CTAACCTCCT CTACTTGAGA GGACATTCCA ATCATAGGCT GCCCATCCAC CCTCTGTGTC
CTCCTGTTAA TTAGGTCACT TAACAAAAAG GAAATTGGGT AGGGGTTTTT CACAGACCGC
TTTCTAAGGG TAATTTTAAA ATATCTGGGA AGTCCCTTCC ACTGCTGTGT TCCAGAAGTG
TTGGTAAACA GCCCACAAAT GTCAACAGCA GAAACATACA AGCTGTCAGC TTTGCACAAG
GGCCCAACAC CCTGCTCATC AAGAAGCACT GTGGTTGCTG TGTTAGTAAT GTGCAAAACA
GGAGGCACAT TTTCCCCACC TGTGTAGGTT CCAAAATATC TAGTGTTTTC ATTTTTACTT
GGATCAGGAA CCCAGCACTC CACTGGATAA GCATTATCCT TATCCAAAAC AGCCTTGTGG
TCAGTGTTCA TCTGCTGACT GTCAACTGTA GCATTTTTTG GGGTTACAGT TTGAGCAGGA
TATTTGGTCC TGTAGTTTGC TAACACACCC TGCAGCTCCA AAGGTTCCCC ACCAACAGCA
AAAAAATGAA AATTTGACCC TTGAATGGGT TTTCCAGCAC CATTTTCATG AGTTTTTTGT
GTCCCTGAAT GCAAGTTTAA CATAGCAGTT ACCCCAATAA CCTCAGTTTT AACAGTAACA
GCTTCCCACA TCAAAATATT TCCACAGGTT AAGTCCTCAT TTAAATTAGG CAAAGGAATT
CCTCGACCTG CAGCCCAAGC TTGGCACTGG CGCCAGAAAT CCGCGCGGTG GTTTTTGGGG
GTCGGGGGTG TTTGGCAGCC ACAGACGCCC GGTGTTCGTG TCGCGCCAGT ACATGCGGTC
CATGCCCAGG CCATCCAAAA ACCATGGGTC TGTCTGCTCA GTCCAGTCGT GGACCTGACC
CCACGCAACG CCCAAAAGAA TAACCCCCAC GAACCATAAA CCATTCCCCA TGGGGGACCC
CGTCCCTAAC CCACGGGGCC CGTGGCTATG GCGGGCTTGC CGCCCCGACG TTGGCTGCGA
GCCCTGGGCC TTCACCCGAA CTTGGGGGTT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT
ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC
CGCGTTTATG AACAAACGAC CCAACACCCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC
ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCATCTC
CCGATCCCCA CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC
ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG TCCCGGCTCC
GGATCGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT GCATCATCGA AATTGCCGTC
AACCAAGCTC TGATAGAGTT GGTCAAGACC AATGCGGAGC ATATACGCCC GGAGCCGCGG
CGATCCTGCA AGCTCCGGAT GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC
CAACCACGGC CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC
CTCGCTCCAG TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT TGTTGGAGCC
GAAATCCGCG TGCACGAGGT GCCGGACTTC GGGGCAGTCC TCGGCCCAAA GCATCAGCTC
ATCGAGAGCC TGCGCGACGG ACGCACTGAC GGTGTCGTCC ATCACAGTTT GCCAGTGATA
CACATGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC
TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC
CATGGCCTCC GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA
CGTGACACCC TGTGCACGGC GGGAGATGCA ATAGGTCAGG CTCTCGCTGA ATTCCCCAAT
GTCAAGCACT TCCGGAATCG GGAGCGCGGC CGATGCAAAG TGCCGATAAA CATAACGATC
TTTGTAGAAA CCATCGGCGC AGCTATTTAC CCGCAGGACA TATCCACGCC CTCCTACATC
GAAGCTGAAA GCACGAGATT CTTCGCCCTC CGAGAGCTGC ATCAGGTCGG AGACGCTGTC
GAACTTTTCG ATCAGAAACT TCTCGACAGA CGTCGCGGTG AGTTCAGGCT TTTTCATATC
AAGCTGATCT TGCGGCACGC TGTTGACGCT GTTAAGCGGG TCGCTGCAGG GTCGCTCGGT
GTTCGAGGCC ACACGCGTCA CCTTAATATG CGAAGTGGAC CTGGGACCGC GCCGCCCCGA
CTGCATCTGC GTGTTCGAAT TCGCCAATGA CAAGACGCTG GGCGGGGTTT GTGTCATCAT
AGAACTAAAG ACATGCAAAT ATATTTCTTC CGGGGACACC GCCAGCAAAC GCGAGCAACG
GGCCACGGGG ATGAAGCAGC CCGGCGGCAC CTCGCTAACG GATTCACCAC TCCAAGAATT
GGAGCCAATC AATTCTTGCG GAGAACTGTG AATGCGCAAA CCAACCCTTG GCAGAACATA
TCCATCGCGT CCGCCATCTC CAGCAGCCGC ACGCGGCGCA TCTCGGGGCC GACGCGCTGG
GCTACGTCTT GCTGGCGTTC GCGACGCGAG GCTGGATGGC CTTCCCCATT ATGATTCTTC
TCGCTTCCGG CGGCATCGGG ATGCCCGCGT TGCAGGCCAT GCTGTCCAGG CAGGTAGATG
ACGACCATCA GGGACAGCTT CAAGGATCGC TCGCGGCTCT TACCAGCGCC AGCAAAAGGC
CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT
TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTTCAAGA
ATTCTCATGT TTGACAGCTT ATCATC
其他哺乳动物表达载体：
