pGRN145

ꄴ上一个： pcDNA3.1/His C

ꄲ下一个： pGL4.30

pGRN145

编号	载体名称
北京华越洋生物VECT6047	pGRN145

pGRN145载体基本信息

载体名称:	pGRN145
质粒类型:	哺乳细胞表达载体；基因载体
高拷贝/低拷贝:	--
插入基因:	hTERT（人类端粒酶反转录酶)
克隆方法:	多克隆位点，限制性内切酶
载体大小:	14002bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氯霉素
筛选标记:
备注:	宿主菌：E. coli GC10；pGRN145载体是表达人类端粒酶反转录酶Telomerase reverse transcriptase的载体。
稳定性:	--
组成型:	组成型
病毒/非病毒:	非病毒

pGRN145载体质粒图谱和多克隆位点信息

pGRN145载体特征

hTERT: 4225-7623

HygB: 9216-10238 (complementary)

CatR: 10822-11480

AmpS: 12941-13799 (complementary)

PAC: 368-964 (complementary)

F1 ori: 11501-11685 (complementary)

MPSV: 3643-4119 (promoter)

Ori: 12165-12747

插入基因：

Gene: Telomerase reverse transcriptase (TERT)

Source: Human

Insert size (kb): 3.398

GenBank accession: NM_003219

Medium 2585: LB Medium (ATCC medium 1065) with 37 mcg/ml chloramphenicol

Restriction digests of this clone gave the following sizes (in kb): HindIII/KpnI - 10.5, 3.5; EcoRI - 10.6, 3.4; XhoI - 12.0, 2.0.

其他哺乳动物表达载体：

pEBVHis A	pcDNA5/TO	pDsRed2-Bid	pNFκB-MetLuc2-Reporter
pGL4.10	pBApo-CMV-neo	pAcGFP1-N1	pEF1α-IRES-DsRed-Express2
pGL4.29	pDsRed-Monomer	pSecTag2 A	pCMV-DsRed-Express2
pGL4.13	pIRES	pGL4.27	pcDNA3.1/NT-GFP-TOPO
pG5 luciferase	pIRES-hrGFP-1a	pGL4.26	pEF1α-IRES-ZsGreen1
pCMV-AD	pDsRed-Express2-N1	pACT	pCMV-Tag 2A
pRevTet-Off	pCMV-Tag 3B	pBIND-Id Control	pCMV-Tag 5B
pTet-Off	pCRE-hrGFP	pTRE2	pAcGFP1-C In-Fusion Ready
pTRE2-hygro	pDsRED2-Mito	pRevTRE	p3XFLAG-CMV-14
pVgRxR	pAcGFP1-F	pTK-hyg	p3XFLAG-CMV-8
pOPI3CAT	pAcGFP1-C1	pTRE3G-Luc	pFLAG-CMV-2
pBK-RSV	pAsRed2-C1	pSwitch	pcDNA3.3-TOPO
pIRES2-DsRed2	pAsRed2-N1	pcDNA4/His C	pcDNA6.2/cLumio-DEST
pCMV-Myc	pAcGFP1-Lam	c-Flag pcDNA3	pCMV-tdTomato
pCMV-Tag 2C	pAcGFP1-C	pcDNA4/TO/Myc-His A	pAcGFP1-Mito
pCMV-Tag 5A	pSEAP2-Basic	pcDNA6/myc-His B	pAcGFP1-N In-Fusion Ready
pCMV-Tag 3C	pBI-CMV3	pcDNA6/V5-His B	pDsRed-Monomer-N In-Fusion Ready
p3XFLAG-CMV-7	pNFkB-DD-tdTomato	pcDNA6.2/nTC-Tag-DEST	pcDNA4/TO/Myc-His B
p3XFLAG-CMV-9	pcDNA3.1/His A	pOptiVEC-TOPO	pIRES2-EGFP
pFLAG-CMV-4	pEBVHis B	pcDNA5/FRT	pcDNA3.1/His C
pBI-CMV4	pGL4.75	pGL4.30	pcDNA3.1/CT-GFP-TOPO
pcDNA4/His A	pGL4.20	pGL4.19	pEF1α-IRES-AcGFP1
pcDNA4/myc-His B	pCMV-SPORT6	pACT-MyoD	pcDNA3.2/V5/GW/D-TOPO
pcDNA4/HisMax C	pCMV-SPORT6	pCMV-BD	pcDNA4/TO/Myc-His/LacZ
pCMV-Tag 4A	pBIND	pCMV-Tet3G	pcDNA4/HisMax-TOPO
pcDNA6/myc-His C	pBD-NF-κB	pTet on advanced	p3XFLAG-CMV-13
pCMV-Tag 2B	pRevTet-On	pTRE-Tight	p3xFLAG-CMV-10
pGRN145	pTet-On	pIND	pFLAG-CMV-3
pCMV-MEK1	pTRE3G	pGene/V5-His B	pcDNA4/TO/Myc-His C
pCMV-Tag 3A	pcDNA4/TO	pOPRSVI	pcDNA6.2/C-YFP-DEST
pCMVLacI	pcDNA4/His B	pcDNA4/HisMax A	pcDNA6.2/cTC-Tag-DEST
pBI-CMV1	pcDNA4/HisMax B	pIRESpuro3	pcDNA6.2/nGeneBLAzer-DEST
pEF1α-AcGFP1-N1	pcDNA4/myc-His C	pIRESneo3	pCRE-MetLuc2-Reporter
pCMV-LacZ	pcDNA3.1/His B	pIRESneo2	pEF1α-DsRed-Express2
pCMV-Tag 4B	pcDNA6/V5-His C	pcDNA4/myc-His A	pDsRed-Express-C1
pCMV-Tag 5C	pCHO1.0	pCMV-PKA	pEF1α-DsRed-Monomer-N1
plRES2-ZsGreen1	pGL3-Promoter	pAcGFP1-N3	pDD-AmCyan1 Reporter
p3XFLAG-CMV-7.1	pCMV-MEKK1	pcDNA5/FRT/TO	pCRE-DD-AmCyan1
pFLAG-CMV-5a	pFLAG-CMV2	pBApo-CMV-Pur	pIRES2-DsRed-Express2
pBudCE4.1	pAcGFP1-C2	pBApo-EF1α-pur	pDsRed-Express-N1
pREP4	ptdTomato-C1	ptdTomato-N1	pcDNA6.2/nLumio-DEST
pBApo-CMV	pCRE-DD-tdTomato	pAcGFP1-Golgi	pcDNA6/myc-His A
pIRES2-AcGFP1	pAcGFP1-Hyg-C1	pAcGFP1-p53	pcDNA6/V5-His A
pIREShyg3	pAcGFP1-Mem	pAcGFP1-Actin	pcDNA5/FRT/TO-TOPO
pcDNA6/TR	pAcGFP1-C3	pBI-CMV2	pcDNA6.2/V5/GW/D-TOPO
pDsRed-Express2-C1	pTT5	pEF1α-tdTomato	pcDNA6.2/cGeneBLAzer-DEST
pBI-CMV5	pSEAP2-Control	pAmCyan1-C1	pcDNA6.2/nGeneBLAzer-GW/D-TOPO