pGL4.30

pGL4.30

pGL4.30载体基本信息

pGL4.30载体质粒图谱和多克隆位点信息

pGL4.30载体简介

The pGL4.30[luc2P/NFAT-RE/Hygro] Vector(a–f) contains an NFAT response element (NFAT-RE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.

pGL4.30载体序列

ORIGIN

    1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCGGAGGAAA AACTGTTTCA TACAGAAGGC

   61 GTGGAGGAAA AACTGTTTCA TACAGAAGGC GTGGAGGAAA AACTGTTTCA TACAGAAGGC

  121 GTAGATCTAG ACTCTAGAGG GTATATAATG GAAGCTCGAA TTCCAGCTTG GCATTCCGGT

  181 ACTGTTGGTA AAAAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA

  241 AAAACATTAA GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC

  301 AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG

  361 CACATATCGA GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT CGGCTGGCAG

  421 AAGCTATGAA GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC AGCGAGAATA

  481 GCTTGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG

  541 CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG

  601 TCGTATTCGT GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA

  661 TCATACAAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC CAAAGCATGT

  721 ACACCTTCGT GACTTCCCAT TTGCCACCCG GCTTCAACGA GTACGACTTC GTGCCCGAGA

  781 GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC

  841 CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT GCCCGCGACC

  901 CCATCTTCGG CAACCAGATC ATCCCCGACA CCGCTATCCT CAGCGTGGTG CCATTTCACC

  961 ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG GTCGTGCTCA

 1021 TGTACCGCTT CGAGGAGGAG CTATTCTTGC GCAGCTTGCA AGACTATAAG ATTCAATCTG

 1081 CCCTGCTGGT GCCCACACTA TTTAGCTTCT TCGCTAAGAG CACTCTCATC GACAAGTACG

 1141 ACCTAAGCAA CTTGCACGAG ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG

 1201 AGGCCGTGGC CAAACGCTTC CACCTACCAG GCATCCGCCA GGGCTACGGC CTGACAGAAA

 1261 CAACCAGCGC CATTCTGATC ACCCCCGAAG GGGACGACAA GCCTGGCGCA GTAGGCAAGG

 1321 TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG ACTTGGACAC CGGTAAGACA CTGGGTGTGA

 1381 ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC CCATGATCAT GAGCGGCTAC GTTAACAACC

 1441 CCGAGGCTAC AAACGCTCTC ATCGACAAGG ACGGCTGGCT GCACAGCGGC GACATCGCCT

 1501 ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT GAAGAGCCTG ATCAAATACA

 1561 AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGAGCATCCT GCTGCAACAC CCCAACATCT

 1621 TCGACGCCGG GGTCGCCGGC CTGCCCGACG ACGATGCCGG CGAGCTGCCC GCCGCAGTCG

 1681 TCGTGCTGGA ACACGGTAAA ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC

 1741 AGGTTACAAC CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT CGTGGACGAG GTGCCTAAAG

 1801 GACTGACCGG CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT TCTCATTAAG GCCAAGAAGG

 1861 GCGGCAAGAT CGCCGTGAAT TCTCACGGCT TCCCTCCCGA GGTGGAGGAG CAGGCCGCCG

 1921 GCACCCTGCC CATGAGCTGC GCCCAGGAGA GCGGCATGGA TAGACACCCT GCTGCTTGCG

 1981 CCAGCGCCAG GATCAACGTC TAAGGCCGCG ACTCTAGAGT CGGGGCGGCC GGCCGCTTCG

 2041 AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA

 2101 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG

 2161 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT

 2221 GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC

 2281 GTTTGCGTAT TGGGCGCTCT TCCGCTGATC TGCGCAGCAC CATGGCCTGA AATAACCTCT

 2341 GAAAGAGGAA CTTGGTTAGC TACCTTCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT

 2401 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT

 2461 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG

 2521 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG

 2581 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT

 2641 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT

 2701 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC GATTCTTCTG ACACTAGCGC CACCATGAAG

 2761 AAGCCCGAAC TCACCGCTAC CAGCGTTGAA AAATTTCTCA TCGAGAAGTT CGACAGTGTG

 2821 AGCGACCTGA TGCAGTTGTC GGAGGGCGAA GAGAGCCGAG CCTTCAGCTT CGATGTCGGC

 2881 GGACGCGGCT ATGTACTGCG GGTGAATAGC TGCGCTGATG GCTTCTACAA AGACCGCTAC

 2941 GTGTACCGCC ACTTCGCCAG CGCTGCACTA CCCATCCCCG AAGTGTTGGA CATCGGCGAG

 3001 TTCAGCGAGA GCCTGACATA CTGCATCAGT AGACGCGCCC AAGGCGTTAC TCTCCAAGAC

 3061 CTCCCCGAAA CAGAGCTGCC TGCTGTGTTA CAGCCTGTCG CCGAAGCTAT GGATGCTATT

 3121 GCCGCCGCCG ACCTCAGTCA AACCAGCGGC TTCGGCCCAT TCGGGCCCCA AGGCATCGGC

 3181 CAGTACACAA CCTGGCGGGA TTTCATTTGC GCCATTGCTG ATCCCCATGT CTACCACTGG

 3241 CAGACCGTGA TGGACGACAC CGTGTCCGCC AGCGTAGCTC AAGCCCTGGA CGAACTGATG

 3301 CTGTGGGCCG AAGACTGTCC CGAGGTGCGC CACCTCGTCC ATGCCGACTT CGGCAGCAAC

 3361 AACGTCCTGA CCGACAACGG CCGCATCACC GCCGTAATCG ACTGGTCCGA AGCTATGTTC

 3421 GGGGACAGTC AGTACGAGGT GGCCAACATC TTCTTCTGGC GGCCCTGGCT GGCTTGCATG

 3481 GAGCAGCAGA CTCGCTACTT CGAGCGCCGG CATCCCGAGC TGGCCGGCAG CCCTCGTCTG

 3541 CGAGCCTACA TGCTGCGCAT CGGCCTGGAT CAGCTCTACC AGAGCCTCGT GGACGGCAAC

 3601 TTCGACGATG CTGCCTGGGC TCAAGGCCGC TGCGATGCCA TCGTCCGCAG CGGGGCCGGC

 3661 ACCGTCGGTC GCACACAAAT CGCTCGCCGG AGCGCAGCCG TATGGACCGA CGGCTGCGTC

 3721 GAGGTGCTGG CCGACAGCGG CAACCGCCGG CCCAGTACAC GACCGCGCGC TAAGGAGGTA

 3781 GGTCGAGTTT AAACTCTAGA ACCGGTCATG GCCGCAATAA AATATCTTTA TTTTCATTAC

 3841 ATCTGTGTGT TGGTTTTTTG TGTGTTCGAA CTAGATGCTG TCGACCGATG CCCTTGAGAG

 3901 CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA

 3961 TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TTCCGCTTCC

 4021 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA

 4081 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA

 4141 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG

 4201 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG

 4261 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT

 4321 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT

 4381 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC

 4441 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT

 4501 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT

 4561 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC

 4621 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA

 4681 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT

 4741 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT

 4801 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA

 4861 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA

 4921 AGTATATATG AGTAAACTTG GTCTGACAGC GGCCGCAAAT GCTAAACCAC TGCAGTGGTT

 4981 ACCAGTGCTT GATCAGTGAG GCACCGATCT CAGCGATCTG CCTATTTCGT TCGTCCATAG

 5041 TGGCCTGACT CCCCGTCGTG TAGATCACTA CGATTCGTGA GGGCTTACCA TCAGGCCCCA

 5101 GCGCAGCAAT GATGCCGCGA GAGCCGCGTT CACCGGCCCC CGATTTGTCA GCAATGAACC

 5161 AGCCAGCAGG GAGGGCCGAG CGAAGAAGTG GTCCTGCTAC TTTGTCCGCC TCCATCCAGT

 5221 CTATGAGCTG CTGTCGTGAT GCTAGAGTAA GAAGTTCGCC AGTGAGTAGT TTCCGAAGAG

 5281 TTGTGGCCAT TGCTACTGGC ATCGTGGTAT CACGCTCGTC GTTCGGTATG GCTTCGTTCA

 5341 ACTCTGGTTC CCAGCGGTCA AGCCGGGTCA CATGATCACC CATATTATGA AGAAATGCAG

 5401 TCAGCTCCTT AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCGGTG TTGTCGCTCA

 5461 TGGTAATGGC AGCACTACAC AATTCTCTTA CCGTCATGCC ATCCGTAAGA TGCTTTTCCG

 5521 TGACCGGCGA GTACTCAACC AAGTCGTTTT GTGAGTAGTG TATACGGCGA CCAAGCTGCT

 5581 CTTGCCCGGC GTCTATACGG GACAACACCG CGCCACATAG CAGTACTTTG AAAGTGCTCA

 5641 TCATCGGGAA TCGTTCTTCG GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA TTGAGATCCA

 5701 GTTCGATATA GCCCACTCTT GCACCCAGTT GATCTTCAGC ATCTTTTACT TTCACCAGCG

 5761 TTTCGGGGTG TGCAAAAACA GGCAAGCAAA ATGCCGCAAA GAAGGGAATG AGTGCGACAC

 5821 GAAAATGTTG GATGCTCATA CTCGTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT

 5881 ACTAGTACGT CTCTCAAGGA TAAGTAAGTA ATATTAAGGT ACGGGAGGTA TTGGACAGGC

 5941 CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAATCGATA

 6001 GTACTAACAT ACGCTCTCCA TCAAAACAAA ACGAAACAAA ACAAACTAGC AAAATAGGCT

 6061 GTCCCCAGTG CAAGTGCAGG TGCCAGAACA TTTCTCT

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