pGL4.19

pGL4.19

pGL4.19载体基本信息

pGL4.19载体质粒图谱和多克隆位点信息

pGL4.19载体简介

The pGL4.19[luc2CP/Neo] Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed  for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker  for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian  codon usage optimized. This vector is engineered with fewer consensus regulatory sequences for reduced background  and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized  for mammalian expression. The pGL4.19[luc2CP/Neo] Vector is a basic vector with no promoter. However, it contains  a multiple cloning region that allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein  destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater  magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.

pGL4.19载体序列

ORIGIN

    1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC

   61 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT

  121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC

  181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC

  241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG

  301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG

  361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC

  421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC

  481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA

  541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC

  601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC

  661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC

  721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC

  781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC

  841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC

  901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG

  961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC

 1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG

 1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC

 1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC

 1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC

 1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT

 1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC

 1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC

 1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC

 1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG

 1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA

 1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC

 1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG

 1741 ATCGCCGTGA ATTCTGCTTG CAAGAACTGG TTCAGTAGCT TAAGCCACTT TGTGATCCAC

 1801 CTTAACAGCC ACGGCTTCCC TCCCGAGGTG GAGGAGCAGG CCGCCGGCAC CCTGCCCATG

 1861 AGCTGCGCCC AGGAGAGCGG CATGGATAGA CACCCTGCTG CTTGCGCCAG CGCCAGGATC

 1921 AACGTCTAAC TGCAGTCTAG AGTCGGGGCG GCCGGCCGCT TCGAGCAGAC ATGATAAGAT

 1981 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG

 2041 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA

 2101 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA

 2161 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTTTGCG TATTGGGCGC

 2221 TCTTCCGCTG ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG GAACTTGGTT

 2281 AGCTACCTTC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA

 2341 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC

 2401 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA

 2461 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG

 2521 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC

 2581 CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT

 2641 TTGCAAAAAG CTCGATTCTT CTGACACTAG CGCCACCATG ATCGAACAAG ACGGCCTCCA

 2701 TGCTGGCAGT CCCGCAGCTT GGGTCGAACG CTTGTTCGGG TACGACTGGG CCCAGCAGAC

 2761 CATCGGATGT AGCGATGCGG CCGTGTTCCG TCTAAGCGCT CAAGGCCGGC CCGTGCTGTT

 2821 CGTGAAGACC GACCTGAGCG GCGCCCTGAA CGAGCTTCAA GACGAGGCTG CCCGCCTGAG

 2881 CTGGCTGGCC ACCACCGGCG TACCCTGCGC CGCTGTGTTG GATGTTGTGA CCGAAGCCGG

 2941 CCGGGACTGG CTGCTGCTGG GCGAGGTCCC TGGCCAGGAT CTGCTGAGCA GCCACCTTGC

 3001 CCCCGCTGAG AAGGTTTCTA TCATGGCCGA TGCAATGCGG CGCCTGCACA CCCTGGACCC

 3061 CGCTACCTGC CCCTTCGACC ACCAGGCTAA GCATCGGATC GAGCGTGCTC GGACCCGCAT

 3121 GGAGGCCGGC CTGGTGGACC AGGACGACCT GGACGAGGAG CATCAGGGCC TGGCCCCCGC

 3181 TGAACTGTTC GCCCGACTGA AAGCCCGCAT GCCGGACGGT GAGGACCTGG TTGTCACACA

 3241 CGGAGATGCC TGCCTCCCTA ACATCATGGT CGAGAATGGC CGCTTCTCCG GCTTCATCGA

 3301 CTGCGGTCGC CTAGGAGTTG CCGACCGCTA CCAGGACATC GCCCTGGCCA CCCGCGACAT

 3361 CGCTGAGGAG CTTGGCGGCG AGTGGGCCGA CCGCTTCTTA GTCTTGTACG GCATCGCAGC

 3421 TCCCGACAGC CAGCGCATCG CCTTCTACCG CTTGCTCGAC GAGTTCTTTT AATGATCTAG

 3481 AACCGGTCAT GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT

 3541 GTGTGTTCGA ACTAGATGCT GTCGACCGAT GCCCTTGAGA GCCTTCAACC CAGTCAGCTC

 3601 CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT TCTTTATCAT

 3661 GCAACTCGTA GGACAGGTGC CGGCAGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC

 3721 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT

 3781 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA

 3841 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC

 3901 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC

 3961 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG

 4021 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA

 4081 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG

 4141 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC

 4201 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG

 4261 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT

 4321 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT

 4381 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC

 4441 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT

 4501 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT

 4561 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT

 4621 GGTCTGACAG CGGCCGCAAA TGCTAAACCA CTGCAGTGGT TACCAGTGCT TGATCAGTGA

 4681 GGCACCGATC TCAGCGATCT GCCTATTTCG TTCGTCCATA GTGGCCTGAC TCCCCGTCGT

 4741 GTAGATCACT ACGATTCGTG AGGGCTTACC ATCAGGCCCC AGCGCAGCAA TGATGCCGCG

 4801 AGAGCCGCGT TCACCGGCCC CCGATTTGTC AGCAATGAAC CAGCCAGCAG GGAGGGCCGA

 4861 GCGAAGAAGT GGTCCTGCTA CTTTGTCCGC CTCCATCCAG TCTATGAGCT GCTGTCGTGA

 4921 TGCTAGAGTA AGAAGTTCGC CAGTGAGTAG TTTCCGAAGA GTTGTGGCCA TTGCTACTGG

 4981 CATCGTGGTA TCACGCTCGT CGTTCGGTAT GGCTTCGTTC AACTCTGGTT CCCAGCGGTC

 5041 AAGCCGGGTC ACATGATCAC CCATATTATG AAGAAATGCA GTCAGCTCCT TAGGGCCTCC

 5101 GATCGTTGTC AGAAGTAAGT TGGCCGCGGT GTTGTCGCTC ATGGTAATGG CAGCACTACA

 5161 CAATTCTCTT ACCGTCATGC CATCCGTAAG ATGCTTTTCC GTGACCGGCG AGTACTCAAC

 5221 CAAGTCGTTT TGTGAGTAGT GTATACGGCG ACCAAGCTGC TCTTGCCCGG CGTCTATACG

 5281 GGACAACACC GCGCCACATA GCAGTACTTT GAAAGTGCTC ATCATCGGGA ATCGTTCTTC

 5341 GGGGCGGAAA GACTCAAGGA TCTTGCCGCT ATTGAGATCC AGTTCGATAT AGCCCACTCT

 5401 TGCACCCAGT TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCGGGGT GTGCAAAAAC

 5461 AGGCAAGCAA AATGCCGCAA AGAAGGGAAT GAGTGCGACA CGAAAATGTT GGATGCTCAT

 5521 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG TCTCTCAAGG

 5581 ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG CCGCAATAAA ATATCTTTAT

 5641 TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT AGTACTAACA TACGCTCTCC

 5701 ATCAAAACAA AACGAAACAA AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG

 5761 GTGCCAGAAC ATTTCTCT

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