pACT-MyoD
pACT-MyoD
编号 | 载体名称 |
北京华越洋生物VECT6044 | pACT-MyoD |
pACT MyoD载体基本信息
载体名称: | pACT-MyoD |
质粒类型: | 哺乳细胞杂交系统载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7400bp |
5' 测序引物及序列: | T7 -F |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | Ampicillin |
筛选标记: | Neomycin |
备注: | -- |
稳定性: | 瞬表达 |
组成型: | 组成型 |
病毒/非病毒: | 非病毒 |
pACT MyoD载体质粒图谱和多克隆位点信息
pACT MyoD载体描述
MyoD is expressed in skeletal muscle and is a myogenic regulatory protein (14,15). The Id protein acts as a negative regulator of myogenic differentiation (16). MyoD and Id are members of the helix-loop-helix family of nuclear proteins. The pBIND-Id Control Vector contains a segment of murine Id cDNA (amino acids 29–148; 13,16) cloned in-frame with the GAL4 DNA-binding domain of the pBIND Vector (Figure 6). The pACT-MyoD Control Vector contains a segment of murine MyoD cDNA (amino acids 1–318; 13,15) cloned in-frame with the VP16 activation domain of the pACT Vector.
其他哺乳动物表达载体:
pcDNA6.2/nGeneBLAzer-GW/D-TOPO |