北京华越洋生物

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首页 ꄲ 载体及质粒 ꄲ pGL3-Promoter

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pGL3-Promoter

ꄴ上一个： pAcGFP1-N3

ꄲ下一个： pEF1α-tdTomato

pGL3-Promoter

编号	载体名称
北京华越洋生物VECT6010	pGL3-Promoter

pGL3-Promoter载体基本信息

载体名称:	pGL3-promoter, pGL3promoter
质粒类型:	荧光素酶报告系统载体
高拷贝/低拷贝:	高拷贝
启动子:	SV40
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5010bp
5' 测序引物及序列:	RV primer3:CTAGCAAAATAGGCTGTCCC
3' 测序引物及序列:	GLprimer2: CTTTATGTTTTTGGCGTCTTCCA
载体标签:	--
载体抗性:	氨苄
筛选标记:	--
备注:	用于快速定量评估影响哺乳动物细胞特定基因表达的因子及其影响能力。
稳定性:	稳定
组成型:	非组成型
病毒/非病毒:	非病毒

pGL3-Promoter载体质粒图谱和多克隆位点信息

pGL3-Promoter载体序列

ORIGIN

1 GGTACCGAGC TCTTACGCGT GCTAGCCCGG GCTCGAGATC TGCGATCTGC ATCTCAATTA

61 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC

121 CGCCCATTCT CCGCCCCATC GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC

181 CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG

241 CAAAAAGCTT GGCATTCCGG TACTGTTGGT AAAGCCACCA TGGAAGACGC CAAAAACATA

301 AAGAAAGGCC CGGCGCCATT CTATCCGCTG GAAGATGGAA CCGCTGGAGA GCAACTGCAT

361 AAGGCTATGA AGAGATACGC CCTGGTTCCT GGAACAATTG CTTTTACAGA TGCACATATC

421 GAGGTGGACA TCACTTACGC TGAGTACTTC GAAATGTCCG TTCGGTTGGC AGAAGCTATG

481 AAACGATATG GGCTGAATAC AAATCACAGA ATCGTCGTAT GCAGTGAAAA CTCTCTTCAA

541 TTCTTTATGC CGGTGTTGGG CGCGTTATTT ATCGGAGTTG CAGTTGCGCC CGCGAACGAC

601 ATTTATAATG AACGTGAATT GCTCAACAGT ATGGGCATTT CGCAGCCTAC CGTGGTGTTC

661 GTTTCCAAAA AGGGGTTGCA AAAAATTTTG AACGTGCAAA AAAAGCTCCC AATCATCCAA

721 AAAATTATTA TCATGGATTC TAAAACGGAT TACCAGGGAT TTCAGTCGAT GTACACGTTC

781 GTCACATCTC ATCTACCTCC CGGTTTTAAT GAATACGATT TTGTGCCAGA GTCCTTCGAT

841 AGGGACAAGA CAATTGCACT GATCATGAAC TCCTCTGGAT CTACTGGTCT GCCTAAAGGT

901 GTCGCTCTGC CTCATAGAAC TGCCTGCGTG AGATTCTCGC ATGCCAGAGA TCCTATTTTT

961 GGCAATCAAA TCATTCCGGA TACTGCGATT TTAAGTGTTG TTCCATTCCA TCACGGTTTT

1021 GGAATGTTTA CTACACTCGG ATATTTGATA TGTGGATTTC GAGTCGTCTT AATGTATAGA

1081 TTTGAAGAAG AGCTGTTTCT GAGGAGCCTT CAGGATTACA AGATTCAAAG TGCGCTGCTG

1141 GTGCCAACCC TATTCTCCTT CTTCGCCAAA AGCACTCTGA TTGACAAATA CGATTTATCT

1201 AATTTACACG AAATTGCTTC TGGTGGCGCT CCCCTCTCTA AGGAAGTCGG GGAAGCGGTT

1261 GCCAAGAGGT TCCATCTGCC AGGTATCAGG CAAGGATATG GGCTCACTGA GACTACATCA

1321 GCTATTCTGA TTACACCCGA GGGGGATGAT AAACCGGGCG CGGTCGGTAA AGTTGTTCCA

1381 TTTTTTGAAG CGAAGGTTGT GGATCTGGAT ACCGGGAAAA CGCTGGGCGT TAATCAAAGA

1441 GGCGAACTGT GTGTGAGAGG TCCTATGATT ATGTCCGGTT ATGTAAACAA TCCGGAAGCG

1501 ACCAACGCCT TGATTGACAA GGATGGATGG CTACATTCTG GAGACATAGC TTACTGGGAC

1561 GAAGACGAAC ACTTCTTCAT CGTTGACCGC CTGAAGTCTC TGATTAAGTA CAAAGGCTAT

1621 CAGGTGGCTC CCGCTGAATT GGAATCCATC TTGCTCCAAC ACCCCAACAT CTTCGACGCA

1681 GGTGTCGCAG GTCTTCCCGA CGATGACGCC GGTGAACTTC CCGCCGCCGT TGTTGTTTTG

1741 GAGCACGGAA AGACGATGAC GGAAAAAGAG ATCGTGGATT ACGTCGCCAG TCAAGTAACA

1801 ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG TTTGTGGACG AAGTACCGAA AGGTCTTACC

1861 GGAAAACTCG ACGCAAGAAA AATCAGAGAG ATCCTCATAA AGGCCAAGAA GGGCGGAAAG

1921 ATCGCCGTGT AATTCTAGAG TCGGGGCGGC CGGCCGCTTC GAGCAGACAT GATAAGATAC

1981 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA

2041 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC

2101 AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC

2161 AAGTAAAACC TCTACAAATG TGGTAAAATC GATAAGGATC CGTCGACCGA TGCCCTTGAG

2221 AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT

2281 TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT

2341 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT

2401 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG

2461 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA

2521 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC

2581 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG

2641 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC

2701 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG

2761 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC

2821 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA

2881 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG

2941 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA

3001 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG

3061 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT

3121 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT

3181 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT

3241 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA

3301 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA

3361 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC

3421 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA

3481 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG

3541 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG

3601 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG

3661 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG

3721 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC

3781 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT

3841 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA

3901 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA

3961 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA

4021 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC

4081 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC

4141 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG

4201 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC

4261 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA

4321 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG

4381 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT

4441 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG

4501 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA

4561 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT

4621 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT

4681 TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTG CCATTCGCCA TTCAGGCTGC

4741 GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CCCAAGCTAC

4801 CATGATAAGT AAGTAATATT AAGGTACGGG AGGTACTTGG AGCGGCCGCA ATAAAATATC

4861 TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC TAACATACGC

4921 TCTCCATCAA AACAAAACGA AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG

4981 TGCAGGTGCC AGAACATTTC TCTATCGATA

其他哺乳动物表达载体：

pEBVHis A	pcDNA5/TO	pDsRed2-Bid	pNFκB-MetLuc2-Reporter
pGL4.10	pBApo-CMV-neo	pAcGFP1-N1	pEF1α-IRES-DsRed-Express2
pGL4.29	pDsRed-Monomer	pSecTag2 A	pCMV-DsRed-Express2
pGL4.13	pIRES	pGL4.27	pcDNA3.1/NT-GFP-TOPO
pG5 luciferase	pIRES-hrGFP-1a	pGL4.26	pEF1α-IRES-ZsGreen1
pCMV-AD	pDsRed-Express2-N1	pACT	pCMV-Tag 2A
pRevTet-Off	pCMV-Tag 3B	pBIND-Id Control	pCMV-Tag 5B
pTet-Off	pCRE-hrGFP	pTRE2	pAcGFP1-C In-Fusion Ready
pTRE2-hygro	pDsRED2-Mito	pRevTRE	p3XFLAG-CMV-14
pVgRxR	pAcGFP1-F	pTK-hyg	p3XFLAG-CMV-8
pOPI3CAT	pAcGFP1-C1	pTRE3G-Luc	pFLAG-CMV-2
pBK-RSV	pAsRed2-C1	pSwitch	pcDNA3.3-TOPO
pIRES2-DsRed2	pAsRed2-N1	pcDNA4/His C	pcDNA6.2/cLumio-DEST
pCMV-Myc	pAcGFP1-Lam	c-Flag pcDNA3	pCMV-tdTomato
pCMV-Tag 2C	pAcGFP1-C	pcDNA4/TO/Myc-His A	pAcGFP1-Mito
pCMV-Tag 5A	pSEAP2-Basic	pcDNA6/myc-His B	pAcGFP1-N In-Fusion Ready
pCMV-Tag 3C	pBI-CMV3	pcDNA6/V5-His B	pDsRed-Monomer-N In-Fusion Ready
p3XFLAG-CMV-7	pNFkB-DD-tdTomato	pcDNA6.2/nTC-Tag-DEST	pcDNA4/TO/Myc-His B
p3XFLAG-CMV-9	pcDNA3.1/His A	pOptiVEC-TOPO	pIRES2-EGFP
pFLAG-CMV-4	pEBVHis B	pcDNA5/FRT	pcDNA3.1/His C
pBI-CMV4	pGL4.75	pGL4.30	pcDNA3.1/CT-GFP-TOPO
pcDNA4/His A	pGL4.20	pGL4.19	pEF1α-IRES-AcGFP1
pcDNA4/myc-His B	pCMV-SPORT6	pACT-MyoD	pcDNA3.2/V5/GW/D-TOPO
pcDNA4/HisMax C	pCMV-SPORT6	pCMV-BD	pcDNA4/TO/Myc-His/LacZ
pCMV-Tag 4A	pBIND	pCMV-Tet3G	pcDNA4/HisMax-TOPO
pcDNA6/myc-His C	pBD-NF-κB	pTet on advanced	p3XFLAG-CMV-13
pCMV-Tag 2B	pRevTet-On	pTRE-Tight	p3xFLAG-CMV-10
pGRN145	pTet-On	pIND	pFLAG-CMV-3
pCMV-MEK1	pTRE3G	pGene/V5-His B	pcDNA4/TO/Myc-His C
pCMV-Tag 3A	pcDNA4/TO	pOPRSVI	pcDNA6.2/C-YFP-DEST
pCMVLacI	pcDNA4/His B	pcDNA4/HisMax A	pcDNA6.2/cTC-Tag-DEST
pBI-CMV1	pcDNA4/HisMax B	pIRESpuro3	pcDNA6.2/nGeneBLAzer-DEST
pEF1α-AcGFP1-N1	pcDNA4/myc-His C	pIRESneo3	pCRE-MetLuc2-Reporter
pCMV-LacZ	pcDNA3.1/His B	pIRESneo2	pEF1α-DsRed-Express2
pCMV-Tag 4B	pcDNA6/V5-His C	pcDNA4/myc-His A	pDsRed-Express-C1
pCMV-Tag 5C	pCHO1.0	pCMV-PKA	pEF1α-DsRed-Monomer-N1
plRES2-ZsGreen1	pGL3-Promoter	pAcGFP1-N3	pDD-AmCyan1 Reporter
p3XFLAG-CMV-7.1	pCMV-MEKK1	pcDNA5/FRT/TO	pCRE-DD-AmCyan1
pFLAG-CMV-5a	pFLAG-CMV2	pBApo-CMV-Pur	pIRES2-DsRed-Express2
pBudCE4.1	pAcGFP1-C2	pBApo-EF1α-pur	pDsRed-Express-N1
pREP4	ptdTomato-C1	ptdTomato-N1	pcDNA6.2/nLumio-DEST
pBApo-CMV	pCRE-DD-tdTomato	pAcGFP1-Golgi	pcDNA6/myc-His A
pIRES2-AcGFP1	pAcGFP1-Hyg-C1	pAcGFP1-p53	pcDNA6/V5-His A
pIREShyg3	pAcGFP1-Mem	pAcGFP1-Actin	pcDNA5/FRT/TO-TOPO
pcDNA6/TR	pAcGFP1-C3	pBI-CMV2	pcDNA6.2/V5/GW/D-TOPO
pDsRed-Express2-C1	pTT5	pEF1α-tdTomato	pcDNA6.2/cGeneBLAzer-DEST
pBI-CMV5	pSEAP2-Control	pAmCyan1-C1	pcDNA6.2/nGeneBLAzer-GW/D-TOPO