pRS413
pRS413
pRS413
编号 | 载体名称 |
北京华越洋生物VECT2880 | pRS413 |
pRS413载体基本信息
| 出品公司: | -- |
|---|---|
| 载体名称: | pRS413 |
| 质粒类型: | 酿酒酵母载体；酵母着丝粒质粒；穿梭载体 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 高拷贝/低拷贝: | 单拷贝 |
| 启动子: | -- |
| 载体大小: | 4970 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素Ampicillin |
| 筛选标记: | -- |
| 克隆宿主: | XL1-Blue |
| 备注: | 含a beta-gal报告基因；宿主菌株YPH499，YPH500，YPH501 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
pRS413载体质粒图谱和多克隆位点信息
pRS413载体序列
pRS413, 4970 bp version 013001 NOTE: The following sequence has been verified for accuracy at the junctions. The remainder of the sequence has been obtained from existing data. 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 51 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 101 TCAGGGCGCG TCAGCGCGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG 151 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAATT CCCGTTTTAA 201 GAGCTTGGTG AGCGCTAGGA GTCACTGCCA GGTATCGTTT GAACACGGCA 251 TTAGTCAGGG AAGTCATAAC ACAGTCCTTT CCCGCAATTT TCTTTTTCTA 301 TTACTCTTGG CCTCCTCTAG TACACTCTAT ATTTTTTTAT GCCTCGGTAA 351 TGATTTTCAT TTTTTTTTTT CCCCTAGCGG ATGACTCTTT TTTTTTCTTA 401 GCGATTGGCA TTATCACATA ATGAATTATA CATTATATAA AGTAATGTGA 451 TTTCTTCGAA GAATATACTA AAAAATGAGC AGGCAAGATA AACGAAGGCA 501 AAGATGACAG AGCAGAAAGC CCTAGTAAAG CGTATTACAA ATGAAACCAA 551 GATTCAGATT GCGATCTCTT TAAAGGGTGG TCCCCTAGCG ATAGAGCACT 601 CGATCTTCCC AGAAAAAGAG GCAGAAGCAG TAGCAGAACA GGCCACACAA 651 TCGCAAGTGA TTAACGTCCA CACAGGTATA GGGTTTCTGG ACCATATGAT 701 ACATGCTCTG GCCAAGCATT CCGGCTGGTC GCTAATCGTT GAGTGCATTG 751 GTGACTTACA CATAGACGAC CATCACACCA CTGAAGACTG CGGGATTGCT 801 CTCGGTCAAG CTTTTAAAGA GGCCCTACTG GCGCGTGGAG TAAAAAGGTT 851 TGGATCAGGA TTTGCGCCTT TGGATGAGGC ACTTTCCAGA GCGGTGGTAG 901 ATCTTTCGAA CAGGCCGTAC GCAGTTGTCG AACTTGGTTT GCAAAGGGAG 951 AAAGTAGGAG ATCTCTCTTG CGAGATGATC CCGCATTTTC TTGAAAGCTT 1001 TGCAGAGGCT AGCAGAATTA CCCTCCACGT TGATTGTCTG CGAGGCAAGA 1051 ATGATCATCA CCGTAGTGAG AGTGCGTTCA AGGCTCTTGC GGTTGCCATA 1101 AGAGAAGCCA CCTCGCCCAA TGGTACCAAC GATGTTCCCT CCACCAAAGG 1151 TGTTCTTATG TAGTGACACC GATTATTTAA AGCTGCAGCA TACGATATAT 1201 ATACATGTGT ATATATGTAT ACCTATGAAT GTCAGTAAGT ATGTATACGA 1251 ACAGTATGAT ACTGAAGATG ACAAGGTAAT GCATCATTCT ATACGTGTCA 1301 TTCTGAACGA GGCGCGCTTT CCTTTTTTCT TTTTGCTTTT TCTTTTTTTT 1351 TCTCTTGAAC TCGACGGATC TATGCGGTGT GAAATACCGC ACAGATGCGT 1401 AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA 1451 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG 1501 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG 1551 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 1601 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG 1651 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA 1701 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC 1751 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA 1801 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC 1851 GCGCTTAATG CGCCGCTACA GGGCGCGTCG CGCCATTCGC CATTCAGGCT 1901 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC 1951 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA 2001 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA 2051 ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCCCC CTCGAGGTCG 2101 ACGGTATCGA TAAGCTTGAT ATCGAATTCC TGCAGCCCGG GGGATCCACT 2151 AGTTCTAGAG CGGCCGCCAC CGCGGTGGAG CTCCAGCTTT TGTTCCCTTT 2201 AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 2251 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAG GAGCCGGAAG 2301 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGGTAA CTCACATTAA 2351 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG 2401 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 2451 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 2501 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 2551 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 2601 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 2651 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 2701 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 2751 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 2801 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 2851 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 2901 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 2951 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 3001 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 3051 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 3101 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 3151 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 3201 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 3251 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 3301 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 3351 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 3401 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 3451 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 3501 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 3551 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 3601 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 3651 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 3701 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 3751 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 3801 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 3851 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 3901 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 3951 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 4001 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 4051 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 4101 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 4151 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 4201 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 4251 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 4301 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 4351 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGGGT CCTTTTCATC 4401 ACGTGCTATA AAAATAATTA TAATTTAAAT TTTTTAATAT AAATATATAA 4451 ATTAAAAATA GAAAGTAAAA AAAGAAATTA AAGAAAAAAT AGTTTTTGTT 4501 TTCCGAAGAT GTAAAAGACT CTAGGGGGAT CGCCAACAAA TACTACCTTT 4551 TATCTTGCTC TTCCTGCTCT CAGGTATTAA TGCCGAATTG TTTCATCTTG 4601 TCTGTGTAGA AGACCACACA CGAAAATCCT GTGATTTTAC ATTTTACTTA 4651 TCGTTAATCG AATGTATATC TATTTAATCT GCTTTTCTTG TCTAATAAAT 4701 ATATATGTAA AGTACGCTTT TTGTTGAAAT TTTTTAAACC TTTGTTTATT 4751 TTTTTTTCTT CATTCCGTAA CTCTTCTACC TTCTTTATTT ACTTTCTAAA 4801 ATCCAAATAC AAAACATAAA AATAAATAAA CACAGAGTAA ATTCCCAAAT 4851 TATTCCATCA TTAAAAGATA CGAGGCGCGT GTAAGTTACA GGCAAGCGAT 4901 CCGTCCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG 4951 TATCACGAGG CCCTTTCGTC
