pMETαC
pMETαC
pMETαC
编号 | 载体名称 |
北京华越洋生物VECT2780 | pMETαC |
pMETα C载体基本信息
出品公司: | Invitrogen |
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载体名称: | pMETalpha C, pMETα C, pMETalphaC, pMETαC |
质粒类型: | 毕赤酵母蛋白表达载体(methanoloica酵母) |
表达水平: | 高拷贝 |
诱导方法: | 甲醇诱导 |
启动子: | AUG1 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8049 bp |
5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´; alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
载体标签: | C-V5, C-His,N-alpha分泌因子 |
载体抗性: | 氨苄 |
筛选标记: | ADE2 |
备注: | 含有alpha factor酵母分泌信号肽,利用甲醇诱导蛋白在methanoloica酵母中分泌表达蛋白。 |
产品目录号: | -- |
稳定性: | 稳定 Stable |
组成型/诱导型: | 诱导型 |
病毒/非病毒: | 非病毒 |
pMETalpha C载体质粒图谱和多克隆位点信息
pMETalpha C载体序列
LOCUS pMET\alpha\C 8049 bp DNA circular 1-AUG-2013 DEFINITION pMET alpha C. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT VNTNAME|pMET alpha C| FEATURES Location/Qualifiers misc_feature 1106..1130 /vntifkey="21" /label=AUG1_fwd_primer misc_feature complement(1668..1689) /vntifkey="21" /label=AUG1_rev_primer CDS 3091..3666 /vntifkey="4" /label=ORF\frame\1 CDS 3672..4805 /vntifkey="4" /label=ORF\frame\3 CDS 4969..5967 /vntifkey="4" /label=ORF\frame\1 gene complement(6780..7640) /gene="Ampicillin" /vntifkey="60" CDS complement(6780..7640) /vntifkey="4" /label=ORF\frame\2 promoter complement(7682..7710) /vntifkey="30" /label=AmpR_promoter sig_peptide 1197..1463 /vntifkey="94" /label=Alpha\factor\secretion\signal BASE COUNT 2363 a 1741 c 1568 g 2377 t ORIGIN 1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag 61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt 121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac 181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca 241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac 301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa 361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc 421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa 481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg 541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc 601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa 661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct 721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct 781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg 841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa 901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat 961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct 1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt 1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac 1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga 1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag 1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact 1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg 1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc 1441 tcgagaaaag agaggctgaa gcatcgatga attcacgtgg cccagccggc cgtggatcca 1501 cgcgtcgtcg acccgcggcg gccgccagct tactagtagg taagcctatc cctaaccctc 1561 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga tctagtatac 1621 aattctaggg ctgcctgttt ggatattttt ataatttttg agagtttgcc aactaatgtt 1681 tttctcttct atgatattta tcatgtagtt ggatatcttt ttttctttga aaagttttta 1741 tttagttctt tctctctttt ttataatgtt ttcagtttct ttcctttata ttgaaaatac 1801 gatgttcttt gtctttcttt tttaaatcct caattataat gtgtaccgtt tgatcaactt 1861 gtaaaattgt ttgtagtcct gtaatcttaa ttgacgattg gttgatagtt taccccagta 1921 tattgtgcgg ctgagatatt acccgattcg gaagattgat tttttcaaca gacataaacc 1981 cataggagat ttagtacagc gacatatgct aattgcctga taatgctcac taacattttc 2041 tccttttaat ctggtatatg catacaggta tgttgatatg gccttgttga tcaaagctac 2101 gaaagtgtag actatttccc aaacagcccc caaccaccac ctcacacgcg cacactgcga 2161 agtgaaagcg ctcccttatt ttcttctccg ctggttcaaa acaaaaaaga atctaatctg 2221 tagacacacg ccccgtaaaa cccaatcttc atcatcgtaa ttttcagtga cggacgatcg 2281 atcaaccata tagtttgtta gtgactgagc ttcagtagtt gacctcttga agaaataacg 2341 tcttgaacaa catactttat acctaccaac attcgccatc aacaacagtg tgacactgac 2401 ataactttac atccattcat cgacaaaaaa atgtctctag ttcggattca tgcttatggg 2461 ttagctattt cgcccaatgt gtccatctga cattactatt ttgcatttta atttaattag 2521 aacttgacta gcgcactacc agcatatcat ctcatttccg taaataccaa atgtattata 2581 tattgaaagc ttttgaccag gttattataa aagaaacttc atgctcgaaa aagatcattt 2641 cgaaaagttg cctagtttca tgaaatttta aagcagttta tataaatttt accttttgat 2701 gcggaattga ctttttcttg aataatacat aacttttctt aaaagaatca aagacagata 2761 aaatttaaga gatattaaat attagtgaga agccgagaat tttgtaacac caacataaca 2821 ctgacatctt taacaacttt taattatgat acatttctta cgtcatgatt gattattaca 2881 gctatgctga caaatgactc ttgttgcatg gctacgaacc gggtaatact aagtgattga 2941 ctcttgctga ccttttatta agaactaaat ggacaatatt atggagcatt tcatgtataa 3001 attggtgcgt aaaatcgttg gatctctctt ctaagtacat cctactataa caatcaagaa 3061 aaacaagaaa atcggacaaa acaatcaagt atggattcta gaacagttgg tatattagga 3121 gggggacaat tgggacgtat gattgttgag gcagcaaaca ggctcaacat taagacggta 3181 atactagatg ctgaaaattc tcctgccaaa caaataagca actccaatga ccacgttaat 3241 ggctcctttt ccaatcctct tgatatcgaa aaactagctg aaaaatgtga tgtgctaacg 3301 attgagattg agcatgttga tgttcctaca ctaaagaatc ttcaagtaaa acatcccaaa 3361 ttaaaaattt acccttctcc agaaacaatc agattgatac aagacaaata tattcaaaaa 3421 gagcatttaa tcaaaaatgg tatagcagtt acccaaagtg ttcctgtgga acaagccagt 3481 gagacgtccc tattgaatgt tggaagagat ttgggttttc cattcgtctt gaagtcgagg 3541 actttggcat acgatggaag aggtaacttc gttgtaaaga ataaggaaat gattccggaa 3601 gctttggaag tactgaagga tcgtcctttg tcgccgaaaa atgggcacca tttactaaag 3661 aattagcagt catgattgtg agatctgtta acggtttagt gttttcttac ccaattgtag 3721 agactatcca caaggacaat atttgtgact tatgttatgc gcctgctaga gttccggact 3781 ccgttcaact taaggcgaag ttgttggcag aaaatgcaat caaatctttt cccggttgtg 3841 gtatatttgg tgtggaaatg ttctatttag aaacagggga attgcttatt aacgaaattg 3901 ccccaaggcc tcacaactct ggacattata ccattgatgc ttgcgtcact tctcaatttg 3961 aagctcattt gagatcaata ttggatttgc caatgccaaa gaatttcaca tctttctcca 4021 ccattacaac gaacgccatt atgctaaatg ttcttggaga caaacataca aaagataaag 4081 agctagaaac ttgcgaaaga gcattggcga ctccaggttc ctcagtgtac ttatatggaa 4141 aagagtctag acctaacaga aaagtaggtc acataaatat tattgcctcc agtatggcgg 4201 aatgtgaaca aaggctgaac tacattacag gtagaactga tattccaatc aaaatctctg 4261 tcgctcaaaa gttggacttg gaagcaatgg tcaaaccatt ggttggaatc atcatgggat 4321 cagactctga cttgccggta atgtctgccg catgtgcggt tttaaaagat tttggcgttc 4381 catttgaagt gacaatagtc tctgctcata gaactccaca taggatgtca gcatatgcta 4441 tttccgcaag caagcgtgga attaaaacaa ttatcgctgg agctggtggg gctgctcact 4501 tgccaggtat ggtggctgca atgacaccac ttcctgtcat cggtgtgccc gtaaaaggtt 4561 cttgtctaga tggagtagat tctttacatt caattgtgca aatgcctaga ggtgttccag 4621 tagctaccgt cgctattaat aatagtacga acgctgcgct gttggctgtc agactgcttg 4681 gcgcttatga ttcaagttat acaacgaaaa tggaacagtt tttattaaag caagaagaag 4741 aagttcttgt caaagcacaa aagttagaaa ctgtcggtta cgaagcttat ctagaaaaca 4801 agtaatatat aagtttattg atatacttgt acagcaaata attataaaat gatataccta 4861 ttttttaggc tttgttatga ttacatcaaa tgtggacttc atacatagaa atcaacgctt 4921 acaggtgtcc ttttttaaga atttcataca taagatctac tagaatcaat gttaaacctg 4981 ttatcattag aacagccatc tccagaaggt tctatccaag tgaaaacttt ggtcttcaag 5041 ccaaagactg ctaaaacaca aaccccagtc ccaattattg ttgttgctga aacttctact 5101 tctactccgg gtggtgttat agccaaagct tcgggcacta aagagccaag attagcaaac 5161 gatgaattga ccaaccaatt ctttggttct cctgctaaag agttttctat tgccagctta 5221 tcaaacgaac acaaagacgg taaagtcaag atcattattg atgctgggat caaagatgat 5281 gactcgacta attacgttat ttccaacact gttgctaagt tagttttaac cggtaagcaa 5341 ttattaggtt tcttatccaa ctacgaacca accatcgttg attttactgc tgaagctgca 5401 accgctgccc caactccagt tgttaagaaa gaacctgctg catctggtaa gcttgatgat 5461 gccaaattaa ttggtgttac tgctgacaag gaaaaagatt tctccaactg gtactcccaa 5521 attctaatga agggtgaaat gttagactac tacgatgtct ctggttgtta cattatgaga 5581 ccagcttctt acacaatttg ggaaaagatt caatcttggt tcgattcgaa gattaaagaa 5641 gatggtgtta ctaatgctta tttcccaatg tttgtctcat ccaaggtttt agaaaaggaa 5701 aaggaccaca tcgatggttt cgctcctgaa gttgcttggg ttaccagagc tggtaaatct 5761 gaattagaag aaccaattgc tattagacca acttctgaaa ctgttatggg taccctgcag 5821 gggccggcct taattaaggc gcgcccttcc tcgctcactg actcgctgcg ctcggtcgtt 5881 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5941 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 6001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 6481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 6721 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 6781 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 6841 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 6901 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 6961 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 7021 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 7081 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 7141 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 7201 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 7261 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 7321 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 7381 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 7441 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 7501 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 7561 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 7621 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 7681 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 7741 ccgcgcacat ttccccgaaa agtgccacct gtatgcggtg tgaaataccg cacagatgcg 7801 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt 7861 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 7921 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 7981 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc aagggcgatg 8041 gcccactac //