pESC-TRP
pESC-TRP
pESC-TRP
编号 | 载体名称 |
北京华越洋生物VECT2760 | pESC-TRP |
pESC-TRP载体基本信息
| 出品公司: | -- |
|---|---|
| 载体名称: | pESC-TRP, pESC TRP |
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1和GAL10 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6525 bp |
| 5' 测序引物及序列: | GAL1-F: ATTTTCGGTTTGTATTACTTC; GAL10-F: GGTGGTAATGCCATGTAATATG |
| 3' 测序引物及序列: | GALI-R: GTTCTTAATACTAACATAACT GAL10-R: GGCAAGGTAGACAAGCCGACAAC |
| 载体标签: | C-Flag, C-Myc |
| 载体抗性: | 氨苄 |
| 筛选标记: | TRP1 |
| 备注: | 利用半乳糖诱导，可以同时使两个基因在酿酒酵母中表达。 |
| 产品目录号: | -- |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pESC-TRP载体质粒图谱和多克隆位点信息
pESC-TRP载体序列
LOCUS pESC-TRP 6525 bp DNA circular SYN 16-SEP-2005 DEFINITION Cloning vector pESC-TRP, complete sequence. ACCESSION AF063848 VERSION AF063848.1 GI:3228558 SOURCE Cloning vector pESC-TRP. ORGANISM Cloning vector pESC-TRP artificial sequences; vectors. COMMENT This file is created by Vector NTI FEATURES Location/Qualifiers source 1..6525 /organism="Cloning vector pESC-TRP" /mol_type="genomic DNA" /specific_host="Escherichia coli K12" /db_xref="taxon:76665" /vntifkey="98" CDS complement(4126..4983) /vntifkey="4" /label=AmpR rep_origin 5117..6272 /vntifkey="33" /label=2\micron\ori CDS 468..1139 /vntifkey="4" /label=TRP1 /note="yeast TRP1 ORF" terminator complement(1779..1943) /vntifkey="43" /label=ADH1\terminator terminator 2932..3121 /vntifkey="43" /label=CYC1\terminator rep_origin complement(1373..1679) /vntifkey="33" /label=f1\ori promoter 2374..2828 /vntifkey="29" /label=GAL1\promoter promoter complement(2162..2373) /vntifkey="29" /label=GAL10\promoter misc_feature 2870..2905 /vntifkey="21" /label=c-myc\tag misc_feature complement(2092..2118) /vntifkey="21" /label=FLAG\tag misc_feature complement(2074..2157) /vntifkey="21" /label=MCS1 misc_feature 2830..2927 /vntifkey="21" /label=MCS2 rep_origin 3308..3975 /vntifkey="33" /label=pUC\ori BASE COUNT 1845 a 1380 c 1462 g 1838 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 1741 tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg 1801 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac 1861 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa 1921 aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat 1981 ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat 2041 tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctcaga tcttatcgtc 2101 gtcatccttg taatccatcg atactagtgc ggccgccctt tagtgagggt tgaattcgaa 2161 ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata atcatattac 2221 atggcattac caccatatac atatccatat acatatccat atctaatctt acttatatgt 2281 tgtggaaatg taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc 2341 agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg 2401 tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc 2461 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag 2521 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga 2581 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg 2641 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac 2701 tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa 2761 tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga 2821 aaaaaccccg gatccgtaat acgactcact atagggcccg ggcgtcgaca tggaacagaa 2881 gttgatttcc gaagaagacc tcgagtaagc ttggtaccgc ggctagctaa gatccgctct 2941 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt 3001 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg 3061 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa 3121 gatccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 3181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 4081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 4141 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 4201 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 4261 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 4321 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 4381 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 4441 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 4501 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 4561 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 4621 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 4681 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 4741 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 4801 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 4861 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 4921 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 4981 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 5041 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 5101 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga 5161 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg 5221 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa 5281 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg 5341 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa 5401 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg 5461 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa 5521 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg 5581 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta 5641 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat 5701 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat 5761 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa 5821 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag 5881 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat 5941 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt 6001 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg 6061 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga 6121 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac 6181 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga 6241 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg 6301 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc 6361 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct 6421 catccttcaa tgctatcatt tcctttgata ttggatcata ttaagaaacc attattatca 6481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc //
