pMET C
pMET C
pMET C
编号 | 载体名称 |
北京华越洋生物VECT2530 | pMET C |
pMETC载体基本信息
| 载体名称: | pMET C, pMETC |
|---|---|
| 质粒类型: | 毕赤酵母蛋白表达载体（methanoloica酵母） |
| 表达水平: | 高拷贝 |
| 诱导方法: | 甲醇诱导 |
| 启动子: | AUG1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7779 bp |
| 5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´ |
| 3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
| 载体标签: | C-V5， C-His |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 利用甲醇诱导蛋白在methanoloica酵母中表达。 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pMETC载体质粒图谱和多克隆位点信息
pMETC载体序列
LOCUS pMET C 7779 bp DNA circular SYN DEFINITION pMET C ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI FEATURES Location/Qualifiers source 1..7779 /organism="pMET C" /mol_type="other DNA" misc_feature 1106..1130 /label="AUG1_fwd_primer" misc_feature complement(1398..1419) /label="AUG1_rev_primer" CDS 2821..3396 /label="ORF frame 1" CDS 3402..4535 /label="ORF frame 3" CDS 4699..5697 /label="ORF frame 1" gene complement(6510..7370) /label="Ampicillin" /gene="Ampicillin" CDS complement(6510..7370) /label="ORF frame 2" promoter complement(7412..7440) /label="AmpR_promoter" ORIGIN 1 GGCGCGCCTT AATTAAGGCC GGCCCCTGCA GGGGGCCCCG AGATGGTACA TACTTAAAAG 61 CTGCCATATT GAGGAACTTC AAAGTTTTAT CTGTTTTTAG AATTAAAAGA CGATTGTTGT 121 AACAAAACGT TGTGCCTACA TAAACTCAAA TTAATGGAAA TAGCCTGTTT TGAAAAATAC 181 ACCTTCTTAA GTACTGACAA AGTTTTGTTA AATGACTATC GAACAAGCCA TGAAATAGCA 241 CATTTCTGCC AGTCACTTTT AACACTTTCC TGCTTGCTGG TTGACTCTCC TCATACAAAC 301 ACCCAAAAGG GAAACTTTCA GTGTGGGGAC ACTTGACATC TCACATGCAC CCCAGATTAA 361 TTTCCCCAGA CGATGCGGAG ACAAGACAAA ACAACCCTTT GTCCTGCTCT TTTCTTTCTC 421 ACACCGCGTG GGTGTGTGCG CAGGCAGGCA GGCAGGCAGC GGGCTGCCTG CCATCTCTAA 481 TCGCTGCTCC TCCCCCCTGG CTTCAAATAA CAGCCTGCTG CTATCTGTGA CCAGATTGGG 541 ACACCCCCCT CCCCTCCGAA TGATCCATCA CCTTTTGTCG TACTCCGACA ATGATCCTTC 601 CCTGTCATCT TCTGGCAATC AGCTCCTTCA ATAATTAAAT CAAATAAGCA TAAATAGTAA 661 AATCGCATAC AAACGTCATG AAAAGTTTTA TCTCTATGGC CAACGGATAG TCTATCTGCT 721 TAATTCCATC CACTTTGGGA ACCGTTCTCT CTTTACCCCA GATTCTCAAA GCTAATATCT 781 GCCCCTTGTC TATTGTCCTT TCTCCGTGTA CAAGCGGAGC TTTTGCCTCC CATCCTCTTG 841 CTTTGTTTCG GTTATTTTTT TTTTCTTTTG AAACTCTTGG TCAAATCAAA TCAAACAAAA 901 CCAAACCTTC TATTCCATCA GATCAACCTT GTTCAACATT CTATAAATCG ATATAAATAT 961 AACCTTATCC CTCCCTTGTT TTTTACCAAT TAATCAATCT TCAAATTTCA AATATTTTCT 1021 ACTTGCTTTA TTACTCAGTA TTAACATTTG TTTAAACCAA CTATAACTTT TAACTGGCTT 1081 TAGAAGTTTT ATTTAACATC AGTTTCAATT TACATCTTTA TTTATTAACG AAATCTTTAC 1141 GAATTAACTC AATCAAAACT TTTACGAAAA AAAAATCTTA CTATTAAGAG CTCGAGAGGA 1201 ATTCACGTGG CCCAGCCGGC CGTGGATCCA CGCGTCGTCG ACCCGCGGCG GCCGCCAGCT 1261 TACTAGTAGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG CGTACCGGTC 1321 ATCATCACCA TCACCATTGA TCTAGTATAC AATTCTAGGG CTGCCTGTTT GGATATTTTT 1381 ATAATTTTTG AGAGTTTGCC AACTAATGTT TTTCTCTTCT ATGATATTTA TCATGTAGTT 1441 GGATATCTTT TTTTCTTTGA AAAGTTTTTA TTTAGTTCTT TCTCTCTTTT TTATAATGTT 1501 TTCAGTTTCT TTCCTTTATA TTGAAAATAC GATGTTCTTT GTCTTTCTTT TTTAAATCCT 1561 CAATTATAAT GTGTACCGTT TGATCAACTT GTAAAATTGT TTGTAGTCCT GTAATCTTAA 1621 TTGACGATTG GTTGATAGTT TACCCCAGTA TATTGTGCGG CTGAGATATT ACCCGATTCG 1681 GAAGATTGAT TTTTTCAACA GACATAAACC CATAGGAGAT TTAGTACAGC GACATATGCT 1741 AATTGCCTGA TAATGCTCAC TAACATTTTC TCCTTTTAAT CTGGTATATG CATACAGGTA 1801 TGTTGATATG GCCTTGTTGA TCAAAGCTAC GAAAGTGTAG ACTATTTCCC AAACAGCCCC 1861 CAACCACCAC CTCACACGCG CACACTGCGA AGTGAAAGCG CTCCCTTATT TTCTTCTCCG 1921 CTGGTTCAAA ACAAAAAAGA ATCTAATCTG TAGACACACG CCCCGTAAAA CCCAATCTTC 1981 ATCATCGTAA TTTTCAGTGA CGGACGATCG ATCAACCATA TAGTTTGTTA GTGACTGAGC 2041 TTCAGTAGTT GACCTCTTGA AGAAATAACG TCTTGAACAA CATACTTTAT ACCTACCAAC 2101 ATTCGCCATC AACAACAGTG TGACACTGAC ATAACTTTAC ATCCATTCAT CGACAAAAAA 2161 ATGTCTCTAG TTCGGATTCA TGCTTATGGG TTAGCTATTT CGCCCAATGT GTCCATCTGA 2221 CATTACTATT TTGCATTTTA ATTTAATTAG AACTTGACTA GCGCACTACC AGCATATCAT 2281 CTCATTTCCG TAAATACCAA ATGTATTATA TATTGAAAGC TTTTGACCAG GTTATTATAA 2341 AAGAAACTTC ATGCTCGAAA AAGATCATTT CGAAAAGTTG CCTAGTTTCA TGAAATTTTA 2401 AAGCAGTTTA TATAAATTTT ACCTTTTGAT GCGGAATTGA CTTTTTCTTG AATAATACAT 2461 AACTTTTCTT AAAAGAATCA AAGACAGATA AAATTTAAGA GATATTAAAT ATTAGTGAGA 2521 AGCCGAGAAT TTTGTAACAC CAACATAACA CTGACATCTT TAACAACTTT TAATTATGAT 2581 ACATTTCTTA CGTCATGATT GATTATTACA GCTATGCTGA CAAATGACTC TTGTTGCATG 2641 GCTACGAACC GGGTAATACT AAGTGATTGA CTCTTGCTGA CCTTTTATTA AGAACTAAAT 2701 GGACAATATT ATGGAGCATT TCATGTATAA ATTGGTGCGT AAAATCGTTG GATCTCTCTT 2761 CTAAGTACAT CCTACTATAA CAATCAAGAA AAACAAGAAA ATCGGACAAA ACAATCAAGT 2821 ATGGATTCTA GAACAGTTGG TATATTAGGA GGGGGACAAT TGGGACGTAT GATTGTTGAG 2881 GCAGCAAACA GGCTCAACAT TAAGACGGTA ATACTAGATG CTGAAAATTC TCCTGCCAAA 2941 CAAATAAGCA ACTCCAATGA CCACGTTAAT GGCTCCTTTT CCAATCCTCT TGATATCGAA 3001 AAACTAGCTG AAAAATGTGA TGTGCTAACG ATTGAGATTG AGCATGTTGA TGTTCCTACA 3061 CTAAAGAATC TTCAAGTAAA ACATCCCAAA TTAAAAATTT ACCCTTCTCC AGAAACAATC 3121 AGATTGATAC AAGACAAATA TATTCAAAAA GAGCATTTAA TCAAAAATGG TATAGCAGTT 3181 ACCCAAAGTG TTCCTGTGGA ACAAGCCAGT GAGACGTCCC TATTGAATGT TGGAAGAGAT 3241 TTGGGTTTTC CATTCGTCTT GAAGTCGAGG ACTTTGGCAT ACGATGGAAG AGGTAACTTC 3301 GTTGTAAAGA ATAAGGAAAT GATTCCGGAA GCTTTGGAAG TACTGAAGGA TCGTCCTTTG 3361 TCGCCGAAAA ATGGGCACCA TTTACTAAAG AATTAGCAGT CATGATTGTG AGATCTGTTA 3421 ACGGTTTAGT GTTTTCTTAC CCAATTGTAG AGACTATCCA CAAGGACAAT ATTTGTGACT 3481 TATGTTATGC GCCTGCTAGA GTTCCGGACT CCGTTCAACT TAAGGCGAAG TTGTTGGCAG 3541 AAAATGCAAT CAAATCTTTT CCCGGTTGTG GTATATTTGG TGTGGAAATG TTCTATTTAG 3601 AAACAGGGGA ATTGCTTATT AACGAAATTG CCCCAAGGCC TCACAACTCT GGACATTATA 3661 CCATTGATGC TTGCGTCACT TCTCAATTTG AAGCTCATTT GAGATCAATA TTGGATTTGC 3721 CAATGCCAAA GAATTTCACA TCTTTCTCCA CCATTACAAC GAACGCCATT ATGCTAAATG 3781 TTCTTGGAGA CAAACATACA AAAGATAAAG AGCTAGAAAC TTGCGAAAGA GCATTGGCGA 3841 CTCCAGGTTC CTCAGTGTAC TTATATGGAA AAGAGTCTAG ACCTAACAGA AAAGTAGGTC 3901 ACATAAATAT TATTGCCTCC AGTATGGCGG AATGTGAACA AAGGCTGAAC TACATTACAG 3961 GTAGAACTGA TATTCCAATC AAAATCTCTG TCGCTCAAAA GTTGGACTTG GAAGCAATGG 4021 TCAAACCATT GGTTGGAATC ATCATGGGAT CAGACTCTGA CTTGCCGGTA ATGTCTGCCG 4081 CATGTGCGGT TTTAAAAGAT TTTGGCGTTC CATTTGAAGT GACAATAGTC TCTGCTCATA 4141 GAACTCCACA TAGGATGTCA GCATATGCTA TTTCCGCAAG CAAGCGTGGA ATTAAAACAA 4201 TTATCGCTGG AGCTGGTGGG GCTGCTCACT TGCCAGGTAT GGTGGCTGCA ATGACACCAC 4261 TTCCTGTCAT CGGTGTGCCC GTAAAAGGTT CTTGTCTAGA TGGAGTAGAT TCTTTACATT 4321 CAATTGTGCA AATGCCTAGA GGTGTTCCAG TAGCTACCGT CGCTATTAAT AATAGTACGA 4381 ACGCTGCGCT GTTGGCTGTC AGACTGCTTG GCGCTTATGA TTCAAGTTAT ACAACGAAAA 4441 TGGAACAGTT TTTATTAAAG CAAGAAGAAG AAGTTCTTGT CAAAGCACAA AAGTTAGAAA 4501 CTGTCGGTTA CGAAGCTTAT CTAGAAAACA AGTAATATAT AAGTTTATTG ATATACTTGT 4561 ACAGCAAATA ATTATAAAAT GATATACCTA TTTTTTAGGC TTTGTTATGA TTACATCAAA 4621 TGTGGACTTC ATACATAGAA ATCAACGCTT ACAGGTGTCC TTTTTTAAGA ATTTCATACA 4681 TAAGATCTAC TAGAATCAAT GTTAAACCTG TTATCATTAG AACAGCCATC TCCAGAAGGT 4741 TCTATCCAAG TGAAAACTTT GGTCTTCAAG CCAAAGACTG CTAAAACACA AACCCCAGTC 4801 CCAATTATTG TTGTTGCTGA AACTTCTACT TCTACTCCGG GTGGTGTTAT AGCCAAAGCT 4861 TCGGGCACTA AAGAGCCAAG ATTAGCAAAC GATGAATTGA CCAACCAATT CTTTGGTTCT 4921 CCTGCTAAAG AGTTTTCTAT TGCCAGCTTA TCAAACGAAC ACAAAGACGG TAAAGTCAAG 4981 ATCATTATTG ATGCTGGGAT CAAAGATGAT GACTCGACTA ATTACGTTAT TTCCAACACT 5041 GTTGCTAAGT TAGTTTTAAC CGGTAAGCAA TTATTAGGTT TCTTATCCAA CTACGAACCA 5101 ACCATCGTTG ATTTTACTGC TGAAGCTGCA ACCGCTGCCC CAACTCCAGT TGTTAAGAAA 5161 GAACCTGCTG CATCTGGTAA GCTTGATGAT GCCAAATTAA TTGGTGTTAC TGCTGACAAG 5221 GAAAAAGATT TCTCCAACTG GTACTCCCAA ATTCTAATGA AGGGTGAAAT GTTAGACTAC 5281 TACGATGTCT CTGGTTGTTA CATTATGAGA CCAGCTTCTT ACACAATTTG GGAAAAGATT 5341 CAATCTTGGT TCGATTCGAA GATTAAAGAA GATGGTGTTA CTAATGCTTA TTTCCCAATG 5401 TTTGTCTCAT CCAAGGTTTT AGAAAAGGAA AAGGACCACA TCGATGGTTT CGCTCCTGAA 5461 GTTGCTTGGG TTACCAGAGC TGGTAAATCT GAATTAGAAG AACCAATTGC TATTAGACCA 5521 ACTTCTGAAA CTGTTATGGG TACCCTGCAG GGGCCGGCCT TAATTAAGGC GCGCCCTTCC 5581 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA 5641 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 5701 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 5761 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 5821 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 5881 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 5941 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 6001 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 6061 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 6121 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 6181 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 6241 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 6301 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 6361 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 6421 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 6481 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 6541 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 6601 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC 6661 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 6721 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 6781 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 6841 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 6901 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 6961 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 7021 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 7081 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 7141 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 7201 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 7261 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 7321 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 7381 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 7441 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 7501 GTATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT 7561 AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA 7621 CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT 7681 GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA 7741 AGGGCGAAAA ACCGTCTATC AAGGGCGATG GCCCACTAC
pMET C其他酵母表达载体：
