pYES2-NTB
pYES2-NTB
pYES2-NTB
编号 | 载体名称 |
北京华越洋生物VECT2520 | pYES2-NTB |
pYES2-NTB载体基本信息
| 载体名称: | pYES2-NTB, pYES2/NT B |
|---|---|
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6039 bp |
| 5' 测序引物及序列: | T7: TAATACGACTCACTATAGGG |
| 3' 测序引物及序列: | CYC1 Terminator: GTGACATAACTAATTACATGATG |
| 载体标签: | N-His, N-Xpress, C-His, C-V5 |
| 载体抗性: | 氨苄 |
| 筛选标记: | URA3 |
| 备注: | 利用半乳糖诱导蛋白在酿酒酵母中表达。 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pYES2-NTB载体质粒图谱和多克隆位点信息
pYES2-NTB载体简介
pYES2 NT B的是一个6.0 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。
该载体包含以下元素：
1. 酵母GAL1启动子，能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白，同时能够被葡萄糖抑制表达
2.多克隆位点可以使用的很多限制酶切位点，便于基因插入。
3.CYC1终止子能够有效终止mRNA的转录。
4.能够利用URA3基因筛选带有ura3基因型的酵母宿主菌株转化子。
5.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。
6.载体本身带有N端His和Xpress标签，同时载体C端也带有V5抗原表位和His纯化标签。
7.在酵母中，利用载体上的2μ复制子维持质粒的高拷贝。
pYES2-NTB载体序列
LOCUS pYES2/NT\B 6039 bp DNA circular SYN DEFINITION pYES2/NT B ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI FEATURES Location/Qualifiers source 1..6039 /organism="pYES2/NT B" /mol_type="other DNA" promoter 1..451 /label="GAL1_promoter" misc_feature 414..437 /label="GAL1_primer" promoter 475..493 /label="T7_promoter" misc_feature 542..560 /label="Xpress_fwd_primer" misc_feature 543..575 /label="T7_leader" misc_feature 579..602 /label="Xpress_EK" terminator 792..1037 /label="CYC1_terminator" misc_feature complement(801..819) /label="CYC1_primer" misc_feature complement(801..819) /label="pYESTrp_rev_primer" rep_origin complement(1265..1884) /label="pBR322_origin" CDS complement(2039..2899) /label="ORF frame 3" CDS complement(2995..3798) /label="ORF frame 1" gene complement(2998..3798) /label="URA3" /gene="URA3" CDS 3287..3763 /label="ORF frame 2" promoter complement(3800..4025) /label="URA3_promoter" rep_origin 4028..5499 /label="2micron_origin" ORIGIN 1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT 61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA 121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC 181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA 241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT 301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC 361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC 421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC 481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG 541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA 601 AGGTACCTAA GGATCCAGTG TGGTGGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT 661 CGAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 721 TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CCTAGAGGGC 781 CGCATCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC CTCCCCCCAC ATCCGCTCTA 841 ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT TTTTATAGTT 901 ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAGACGC 961 GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG 1021 GCTTTAATTT GCAAGCTGCG GCCCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 1081 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 1141 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 1201 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGCCCAGG AACCGTAAAA 1261 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 1321 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 1381 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 1441 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 1501 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 1561 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 1621 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 1681 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG 1741 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 1801 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 1861 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 1921 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 1981 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 2041 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 2101 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GCGCTTACCA TCTGGCCCCA 2161 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 2221 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 2281 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 2341 TTGTTGGCAT TGCTACAGGC ATCGTGGTGT CACTCTCGTC GTTTGGTATG GCTTCATTCA 2401 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 2461 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 2521 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 2581 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 2641 CTTGCCCGGC GTCAATACGG GATAATAGTG TATCACATAG CAGAACTTTA AAAGTGCTCA 2701 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 2761 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 2821 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 2881 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATGGGT AATAACTGAT ATAATTAAAT 2941 TGAAGCTCTA ATTTGTGAGT TTAGTATACA TGCATTTACT TATAATACAG TTTTTTAGTT 3001 TTGCTGGCCG CATCTTCTCA AATATGCTTC CCAGCCTGCT TTTCTGTAAC GTTCACCCTC 3061 TACCTTAGCA TCCCTTCCCT TTGCAAATAG TCCTCTTCCA ACAATAATAA TGTCAGATCC 3121 TGTAGAGACC ACATCATCCA CGGTTCTATA CTGTTGACCC AATGCGTCTC CCTTGTCATC 3181 TAAACCCACA CCGGGTGTCA TAATCAACCA ATCGTAACCT TCATCTCTTC CACCCATGTC 3241 TCTTTGAGCA ATAAAGCCGA TAACAAAATC TTTGTCGCTC TTCGCAATGT CAACAGTACC 3301 CTTAGTATAT TCTCCAGTAG ATAGGGAGCC CTTGCATGAC AATTCTGCTA ACATCAAAAG 3361 GCCTCTAGGT TCCTTTGTTA CTTCTTCTGC CGCCTGCTTC AAACCGCTAA CAATACCTGG 3421 GCCCACCACA CCGTGTGCAT TCGTAATGTC TGCCCATTCT GCTATTCTGT ATACACCCGC 3481 AGAGTACTGC AATTTGACTG TATTACCAAT GTCAGCAAAT TTTCTGTCTT CGAAGAGTAA 3541 AAAATTGTAC TTGGCGGATA ATGCCTTTAG CGGCTTAACT GTGCCCTCCA TGGAAAAATC 3601 AGTCAAGATA TCCACATGTG TTTTTAGTAA ACAAATTTTG GGACCTAATG CTTCAACTAA 3661 CTCCAGTAAT TCCTTGGTGG TACGAACATC CAATGAAGCA CACAAGTTTG TTTGCTTTTC 3721 GTGCATGATA TTAAATAGCT TGGCAGCAAC AGGACTAGGA TGAGTAGCAG CACGTTCCTT 3781 ATATGTAGCT TTCGACATGA TTTATCTTCG TTTCCTGCAG GTTTTTGTTC TGTGCAGTTG 3841 GGTTAAGAAT ACTGGGCAAT TTCATGTTTC TTCAACACTA CATATGCGTA TATATACCAA 3901 TCTAAGTCTG TGCTCCTTCC TTCGTTCTTC CTTCTGTTCG GAGATTACCG AATCAAAAAA 3961 ATTTCAAAGA AACCGAAATC AAAAAAAAGA ATAAAAAAAA AATGATGAAT TGAATTGAAA 4021 AGCTAGCTTA TCGATGATAA GCTGTCAAAG ATGAGAATTA ATTCCACGGA CTATAGACTA 4081 TACTAGATAC TCCGTCTACT GTACGATACA CTTCCGCTCA GGTCCTTGTC CTTTAACGAG 4141 GCCTTACCAC TCTTTTGTTA CTCTATTGAT CCAGCTCAGC AAAGGCAGTG TGATCTAAGA 4201 TTCTATCTTC GCGATGTAGT AAAACTAGCT AGACCGAGAA AGAGACTAGA AATGCAAAAG 4261 GCACTTCTAC AATGGCTGCC ATCATTATTA TCCGATGTGA CGCTGCAGCT TCTCAATGAT 4321 ATTCGAATAC GCTTTGAGGA GATACAGCCT AATATCCGAC AAACTGTTTT ACAGATTTAC 4381 GATCGTACTT GTTACCCATC ATTGAATTTT GAACATCCGA ACCTGGGAGT TTTCCCTGAA 4441 ACAGATAGTA TATTTGAACC TGTATAATAA TATATAGTCT AGCGCTTTAC GGAAGACAAT 4501 GTATGTATTT CGGTTCCTGG AGAAACTATT GCATCTATTG CATAGGTAAT CTTGCACGTC 4561 GCATCCCCGG TTCATTTTCT GCGTTTCCAT CTTGCACTTC AATAGCATAT CTTTGTTAAC 4621 GAAGCATCTG TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA 4681 ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC 4741 CAACGAAGAA TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGACG AGAGCGCTAA 4801 TTTTTCAAAC AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC 4861 TATTTTACCA ACAAAGAATC TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC 4921 GCTATTTTTC TAACAAAGCA TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG 4981 CAGTCTCTTG ATAACTTTTT GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG 5041 TGTCTATTTT CTCTTCCATA AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA 5101 GCGAAGCTGC GGGTGCATTT TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT 5161 GTGGATTGCG CATACTTTGT GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG 5221 AAAATTATGA ACGGTTTCTT CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA 5281 TTTTCGTATT GTTTTCGATT CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA 5341 GAGTAATACT AGAGATAAAC ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG 5401 AGCGAAAGGT GGATGGGTAG GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA 5461 TACTTTTGAG CAATGTTTGT GGAAGCGGTA TTCGCAATGG GAAGCTCCAC CCCGGTTGAT 5521 AATCAGAAAA GCCCCAAAAA CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT 5581 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACGAATAG 5641 CCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT 5701 GTTCCAGTTT CCAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA 5761 AAAAGGGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG 5821 GGGTCGAGGT GCCGTAAAGC AGTAAATCGG AAGGGTAAAC GGATGCCCCC ATTTAGAGCT 5881 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGG 5941 GCTAGGGCGG TGGGAAGTGT AGGGGTCACG CTGGGCGTAA CCACCACACC CGCCGCGCTT 6001 AATGGGGCGC TACAGGGCGC GTGGGGATGA TCCACTAGT //
pYES2-NTB其他酵母表达载体：
