pYES6/CT
pYES6/CT
pYES6/CT
编号 | 载体名称 |
北京华越洋生物VECT2510 | pYES6/CT |
pYES6-CT载体基本信息
| 载体名称: | pYES6-CT, pYES6/CT |
|---|---|
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5839 bp |
| 5' 测序引物及序列: | GAL1-F: AATATACCTCTATACTTTAACGTC |
| 3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC |
| 载体标签: | C-His, C-V5 |
| 载体抗性: | 氨苄, 杀稻瘟菌素 |
| 筛选标记: | 杀稻瘟菌素(Blasticidin) |
| 备注: | 利用半乳糖诱导蛋白在酿酒酵母中表达。 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pYES6-CT载体质粒图谱和多克隆位点信息
pYES6-CT载体简介
pYES6/CT的是一个5.8 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。
该载体包含以下元素：
1. 酵母GAL1启动子，能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白，同时能够被葡萄糖抑制表达
2.多克隆位点可以使用的10个限制酶切位点，和两个BstX I酶切位点，便于基因插入。
3.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。
4.杀稻瘟菌素(Blasticidin)抗性基因能够方便在大肠杆菌和酵母中的进行载体筛选。
5. 载体C端编码V5抗原表位和His纯化标签，便于蛋白纯化和western blot检测。
6. 在酵母中，利用载体上的2μ复制子维持质粒的高拷贝。
pYES6-CT载体序列
LOCUS pYES6/CT 5839 bp DNA circular SYN DEFINITION pYES6/CT ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI FEATURES Location/Qualifiers source 1..5839 /organism="pYES6/CT" /mol_type="other DNA" promoter 1..451 /label="GAL1_promoter" misc_feature 414..437 /label="GAL1_primer" promoter 475..493 /label="T7_promoter" terminator 716..961 /label="CYC1_terminator" misc_feature complement(725..743) /label="pYESTrp_rev_primer" misc_feature complement(725..743) /label="CYC1_primer" gene complement(1963..2823) /label="Ampicillin" /gene="Ampicillin" CDS complement(1963..2823) /label="ORF frame 2" promoter complement(3341..3408) /label="EM7_promoter" rep_origin 3828..5299 /label="2micron_origin" rep_origin complement(4418..5296) /label="2micron2_origin" ORIGIN 1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT 61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA 121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC 181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA 241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT 301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC 361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC 421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC 481 GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC 541 AGTGTGCTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC 601 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT 661 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCCTAGA GGGCCGCATC ATGTAATTAG 721 TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG 781 TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA 841 CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA 901 TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGGCTTTA ATTTGCAAGC 961 TGCGGCCCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 1021 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 1081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 1141 GAACATGTGA GCAAAAGGCC AGCAAAAGCC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 1201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1621 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 1801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 1861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 1921 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 1981 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 2041 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 2101 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2161 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 2221 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 2281 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2341 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 2401 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 2461 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2521 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 2581 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 2641 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 2701 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 2761 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 2821 CATACTCTTC CTTTTTCAAT GGGTAATAAC TGATATAATT AAATTGAAGC TCTAATTTGT 2881 GAGTTTAGTA GACATGCAGA TCTCCCGGGA CGTGTCAGTC CTGCTCCTCG GCCACGAAGT 2941 GCTTAGCCCT CCCACACATA ACCAGAGGGC AGCAATTCAC GAATCCCAAC TGCCGTCGGC 3001 TGTCCATCAC TGTCCTTCAC TATGGCTTTG ATCCCAGGAT GCAGATCGAG AAGAACCTGT 3061 CGGCACCGTC CGCAGGGCTC AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG 3121 TCAGGTTGCC AGCTGCCGCA GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCGCAAGGTC 3181 CCCCAGTAAA ATGATATACA TTGACACCAG TGAAGATGCG GCCGTCGCTA GAGAGAGCTG 3241 CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGATGCTGTT GATTGTAGCC GTTGCTCTTT 3301 CAATGAGGGT GGATTCTTCT TGAGACAAAG GCTTGGCCAT GGTTTAGTTC CTCACCTTGT 3361 CGTATTATAC TATGCCGATA TACTATGCCG ATGATTAATT GTCAACACCG CCCTTAGATT 3421 AGATTGCTAT GCTTTCTTTC TAATGAGCAA GAAGTAAAAA AAGTTGTAAT AGAACAAGAA 3481 AAATGAAACT GAAACTTGAG AAATTGAAGA CCGTTTATTA ACTTAAATAT CAATGGGAGG 3541 TCATCGAAAG AGAAAAAAAT CAAAAAAAAA AATTTTCAAG AAAAAGAAAC GTGATAAAAA 3601 TTTTTATTGC CTTTTTCGAC GAAGAAAAAG AAACGAGGCG GTCTCTTTTT TCTTTTCCAA 3661 ACCTTTAGTA CGGGTAATTA ACGACACCCT AGAGGAAGAA AGAGGGGAAA TTTAGTATGC 3721 TGTGCTTGGG TGTTTTGAAG TGGTACGGCG ATGCGCGGAG TCCGAGAAAA TCTGGAAGAG 3781 TAAAAAAGGA GTAGAAACAT TTTGAAGCTA TGGTGTGTGG GGCTAGCTTA TCGATGATAA 3841 GCTGTCAAAG ATGAGAATTA ATTCCACGGA CTATAGACTA TACTAGATAC TCCGTCTACT 3901 GTACGATACA CTTCCGCTCA GGTCCTTGTC CTTTAACGAG GCCTTACCAC TCTTTTGTTA 3961 CTCTATTGAT CCAGCTCAGC AAAGGCAGTG TGATCTAAGA TTCTATCTTC GCGATGTAGT 4021 AAAACTAGCT AGACCGAGAA AGAGACTAGA AATGCAAAAG GCACTTCTAC AATGGCTGCC 4081 ATCATTATTA TCCGATGTGA CGCTGCAGCT TCTCAATGAT ATTCGAATAC GCTTTGAGGA 4141 GATACAGCCT AATATCCGAC AAACTGTTTT ACAGATTTAC GATCGTACTT GTTACCCATC 4201 ATTGAATTTT GAACATCCGA ACCTGGGAGT TTTCCCTGAA ACAGATAGTA TATTTGAACC 4261 TGTATAATAA TATATAGTCT AGCGCTTTAC GGAAGACAAT GTATGTATTT CGGTTCCTGG 4321 AGAAACTATT GCATCTATTG CATAGGTAAT CTTGCACGTC GCATCCCCGG TTCATTTTCT 4381 GCGTTTCCAT CTTGCACTTC AATAGCATAT CTTTGTTAAC GAAGCATCTG TGCTTCATTT 4441 TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT 4501 TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC CAACGAAGAA TCTGTGCTTC 4561 ATTTTTGTAA AACAAAAATG CAACGCGACG AGAGCGCTAA TTTTTCAAAC AAAGAATCTG 4621 AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC TATTTTACCA ACAAAGAATC 4681 TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTC TAACAAAGCA 4741 TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG CAGTCTCTTG ATAACTTTTT 4801 GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG TGTCTATTTT CTCTTCCATA 4861 AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA GCGAAGCTGC GGGTGCATTT 4921 TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT GTGGATTGCG CATACTTTGT 4981 GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG AAAATTATGA ACGGTTTCTT 5041 CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA TTTTCGTATT GTTTTCGATT 5101 CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA GAGTAATACT AGAGATAAAC 5161 ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG AGCGAAAGGT GGATGGGTAG 5221 GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA TACTTTTGAG CAATGTTTGT 5281 GGAAGCGGTA TTCGCAATGG GAAGCTCCAC CCCGGTTGAT AATCAGAAAA GCCCCAAAAA 5341 CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC 5401 GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACGAATAG CCCGAAATCG GCAAAATCCC 5461 TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT CCAACAAGAG 5521 TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAAGGGTCT ATCAGGGCGA 5581 TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC 5641 AGTAAATCGG AAGGGTAAAC GGATGCCCCC ATTTAGAGCT TGACGGGGAA AGCCGGCGAA 5701 CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGG GCTAGGGCGG TGGGAAGTGT 5761 AGGGGTCACG CTGGGCGTAA CCACCACACC CGCCGCGCTT AATGGGGCGC TACAGGGCGC 5821 GTGGGGATGA TCCACTAGT //
pYES6/CT其他酵母表达载体：
