pACT2-AD
pACT2-AD
pACT2-AD
编号 | 载体名称 |
北京华越洋生物VECT2400 | pACT2-AD |
pACT2-AD载体基本信息
| 载体名称: | pACT2 AD、pACT2-AD |
|---|---|
| 质粒类型: | 酵母双杂交载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | ADH1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8117bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
pACT2-AD载体质粒图谱和多克隆位点信息
pACT2-AD载体序列
LOCUS pACT2 AD 8117 bp DNA SYN DEFINITION pACT2 AD ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8117 /organism="pACT2 AD" /mol_type="other DNA" gene 10..417 /label="FLP_recombinase (864 - 1271)" /gene="FLP_recombinase (864 - 1271)" rep_origin 420..1580 /label="2micron2_origin" misc_feature 570..617 /label="FRT" rep_origin complement(714..2054) /label="2micron_origin" gene 2462..3568 /label="LEU2" /gene="LEU2" CDS 2462..3568 /label="ORF frame 2" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" misc_feature complement(4186..4219) /label="loxP" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" misc_feature complement(4263..4296) /label="loxP" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" misc_feature complement(4368..4401) /label="loxP" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" terminator complement(4415..4606) /label="yADH1_terminator" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" terminator complement(4429..4743) /label="yADH2_terminator" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" misc_feature 4527..4551 /label="pHybLex_rev_primer" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" misc_feature 4704..4727 /label="pJG4_5_rev_primer" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" CDS complement(4970..5488) /label="ORF frame 2" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" misc_feature complement(5042..5068) /label="HA" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" misc_feature complement(5081..5419) /label="Gal4_AD" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" misc_feature complement(5081..5488) /label="Gal4_AD" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" misc_feature complement(5138..5155) /label="Gal4_AD_primer" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" misc_feature complement(5423..5479) /label="SV40_NLS" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" misc_feature complement(5438..5458) /label="SV40 NLS" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" misc_feature complement(5438..5452) /label="NLS" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" promoter complement(5504..5900) /label="yADH1_promoter" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" misc_feature complement(5934..5956) /label="pGEX_3_primer" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" gene complement(7127..7987) /label="Ampicillin" /gene="Ampicillin" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYASLGGHMAMEAP GIRIRAREIYES*" CDS complement(7127..7987) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(8029..8057) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 GCTTTGAAGA AAAATGCGCC TTATTCAATC TTTGCTATAA AAAATGGCCC AAAATCTCAC 61 ATTGGAAGAC ATTTGATGAC CTCATTTCTT TCAATGAAGG GCCTAACGGA GTTGACTAAT 121 GTTGTGGGAA ATTGGAGCGA TAAGCGTGCT TCTGCCGTGG CCAGGACAAC GTATACTCAT 181 CAGATAACAG CAATACCTGA TCACTACTTC GCACTAGTTT CTCGGTACTA TGCATATGAT 241 CCAATATCAA AGGAAATGAT AGCATTGAAG GATGAGACTA ATCCAATTGA GGAGTGGCAG 301 CATATAGAAC AGCTAAAGGG TAGTGCTGAA GGAAGCATAC GATACCCCGC ATGGAATGGG 361 ATAATATCAC AGGAGGTACT AGACTACCTT TCATCCTACA TAAATAGACG CATATAAGTA 421 CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT GTATATATAT ATACAGGCAA 481 CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT GCGCAGCTCG CGTTGCATTT 541 TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT TCCGAAGTTC CTATTCTCTA 601 GAAAGTATAG GAACTTCAGA GCGCTTTTGA AAACCAAAAG CGCTCTGAAG ACGCACTTTC 661 AAAAAACCAA AAACGCACCG GACTGTAACG AGCTACTAAA ATATTGCGAA TACCGCTTCC 721 ACAAACATTG CTCAAAAGTA TCTCTTTGCT ATATATCTCT GTGCTATATC CCTATATAAC 781 CTACCCATCC ACCTTTCGCT CCTTGAACTT GCATCTAAAC TCGACCTCTA CATTTTTTAT 841 GTTTATCTCT AGTATTACTC TTTAGACAAA AAAATTGTAG TAAGAACTAT TCATAGAGTG 901 AATCGAAAAC AATACGAAAA TGTAAACATT TCCTATACGT AGTATATAGA GACAAAATAG 961 AAGAAACCGT TCATAATTTT CTGACCAATG AAGAATCATC AACGCTATCA CTTTCTGTTC 1021 ACAAAGTATG CGCAATCCAC ATCGGTATAG AATATAATCG GGGATGCCTT TATCTTGAAA 1081 AAATGCACCC GCAGCTTCGC TAGTAATCAG TAAACGCGGG AAGTGGAGTC AGGCTTTTTT 1141 TATGGAAGAG AAAATAGACA CCAAAGTAGC CTTCTTCTAA CCTTAACGGA CCTACAGTGC 1201 AAAAAGTTAT CAAGAGACTG CATTATAGAG CGCACAAAGG AGAAAAAAAG TAATCTAAGA 1261 TGCTTTGTTA GAAAAATAGC GCTCTCGGGA TGCATTTTTG TAGAACAAAA AAGAAGTATA 1321 GATTCTTTGT TGGTAAAATA GCGCTCTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT 1381 CAGATTCTTT GTTTGAAAAA TTAGCGCTCT CGCGTTGCAT TTTTGTTTTA CAAAAATGAA 1441 GCACAGATTC TTCGTTGGTA AAATAGCGCT TTCGCGTTGC ATTTCTGTTC TGTAAAAATG 1501 CAGCTCAGAT TCTTTGTTTG AAAAATTAGC GCTCTCGCGT TGCATTTTTG TTCTACAAAA 1561 TGAAGCACAG ATGCTTCGTT AACAAAGATA TGCTATTGAA GTGCAAGATG GAAACGCAGA 1621 AAATGAACCG GGGATGCGAC GTGCAAGATT ACCTATGCAA TAGATGCAAT AGTTTCTCCA 1681 GGAACCGAAA TACATACATT GTCTTCCGTA AAGCGCTAGA CTATATATTA TTATACAGGT 1741 TCAAATATAC TATCTGTTTC AGGGAAAACT CCCAGGTTCG GATGTTCAAA ATTCAATGAT 1801 GGGTAACAAG TACGATCGTA AATCTGTAAA ACAGTTTGTC GGATATTAGG CTGTATCTCC 1861 TCAAAGCGTA TTCGAATATC ATTGAGAAGC TGCAGCGTCA CATCGGATAA TAATGATGGC 1921 AGCCATTGTA GAAGTGCCTT TTGCATTTCT AGTCTCTTTC TCGGTCTAGC TAGTTTTACT 1981 ACATCGCGAA GATAGAATCT TAGATCACAC TGCCTTTGCT GAGCTGGATC AATAGAGTAA 2041 CAAAAGAGTG GTAAGGAACT GTGGGAATAC TCAGGTATCG TAAGATGCAA GAGTTCGAAT 2101 CTCTTAGCAA CCATTATTTT TTTCCTCAAC ATAACGAGAA CACACAGGGG CGCTATCGCA 2161 CAGAATCAAA TTCGATGACT GGAAATTTTT TGTTAATTTC AGAGGTCGCC TGACGCATAT 2221 ACCTTTTTCA ACTGAAAAAT TGGGAGAAAA AGGAAAGGTG AGAGGCCGGA ACCGGCTTTT 2281 CATATAGAAT AGAGAAGCGT TCATGACTAA ATGCTTGCAT CACAATACTT GAAGTTGACA 2341 ATATTATTTA AGGACCTATT GTTTTTTCCA ATAGGTGGTT AGCAATCGTC TTACTTTCTA 2401 ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA TTTCAAGGAT ATACCATTCT 2461 AATGTCTGCC CCTATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG ACCACGTTGG 2521 TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG TTCGTTCCAA 2581 TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG CTACAGGTGT 2641 CCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGTT GATGCCGTTT TGTTAGGTGC 2701 TGTGGGTGGT CCTAAATGGG GTACCGGTAG TGTTAGACCT GAACAAGGTT TACTAAAAAT 2761 CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT CCGACTCTCT 2821 TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG TTGTTGTCAG 2881 AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG ATGGTGTCGC 2941 TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA TGGCCGCTTT 3001 CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG CTAATGTTTT 3061 GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG AATTTCCTAC 3121 ATTGAAGGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA AGAACCCAAC 3181 CCACCTAAAT GGTATTATAA TCACCAGCAA CATGTTTGGT GATATCATCT CCGATGAAGC 3241 CTCCGTTATC CCAGGTTCCT TGGGTTTGTT GCCATCTGCG TCCTTGGCCT CTTTGCCAGA 3301 CAAGAACACC GCATTTGGTT TGTACGAACC ATGCCACGGT TCTGCTCCAG ATTTGCCAAA 3361 GAATAAGGTT GACCCTATCG CCACTATCTT GTCTGCTGCA ATGATGTTGA AATTGTCATT 3421 GAACTTGCCT GAAGAAGGTA AGGCCATTGA AGATGCAGTT AAAAAGGTTT TGGATGCAGG 3481 TATCAGAACT GGTGATTTAG GTGGTTCCAA CAGTACCACC GAAGTCGGTG ATGCTGTCGC 3541 CGAAGAAGTT AAGAAAATCC TTGCTTAAAA AGATTCTCTT TTTTTATGAT ATTTGTACAT 3601 AAACTTTATA AATGAAATTC ATAATAGAAA CGACACGAAA TTACAAAATG GAATATGTTC 3661 ATAGGGTAGA CGAAACTATA TACGCAATCT ACATACATTT ATCAAGAAGG AGAAAAAGGA 3721 GGATAGTAAA GGAATACAGG TAAGCAAATT GATACTAATG GCTCAACGTG ATAAGGAAAA 3781 AGAATTGCAC TTTAACATTA ATATTGACAA GGAGGAGGGC ACCACACAAA AAGTTAGGTG 3841 TAACAGAAAA TCATGAAACT ACGATTCCTA ATTTGATATT GGAGGATTTT CTCTAAAAAA 3901 AAAAAAATAC AACAAATAAA AAACACTCAA TGACCTGACC ATTTGATGGA GTTTAAGTCA 3961 ATACCTTCTT GAACCATTTC CCATAATGGT GAAAGTTCCC TCAAGAATTT TACTCTGTCA 4021 GAAACGGCCT TAACGACGTA GTCGACCAAT TCCGATCATA TCAATAACCC TTAATATAAC 4081 TTCGTATAAT GTATGCTATC GAAGTTATTA GGTCTGAAGA GGAGTTTACG TCCAGCCAAG 4141 CTAGCCTGGC TGCAGGTCGA GCGGCCGCGA TCCGGAACCC TTAATATAAC TTCGTATAAT 4201 GTATGCTATA CGAAGTTATT AGGTCCCTCG ACCAATTCCG ATCATATTCA ATAACCCTTA 4261 ATATAACTTC GTATAATGTA TGCTATACGA AGTTATTAGG TCTGAAGAGG AGTTTACGTC 4321 CGCTAGCTTG GCTGCAGGTC GAGCGGCCGC GATCCGGAAC CCTTAATATA ACTTCGTATA 4381 ATGTATGCTA TACGAAGTTA TTAGGTCCCT CGCACATGCC GGTAGAGGTG TGGTCAATAA 4441 GAGCGACCTC ATGCTATACC TGAGAAAGCA ACCTGACCTA CAGGAAAGAG TTACTCAAGA 4501 ACAAGAATTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA TTTGTATACA CTTATTTTTT 4561 TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT TTAGAAGTGT 4621 CAACAACGTA TCTACCAACG ATTTGACCCT TTTCCATCTT TTCGTAAATT TCTGGCAAGG 4681 TAGACAAGCC GACAACCTTG ATTGGAGACT TGACCAAACC TCTGGCGAAG AAGTCCAAAG 4741 CTTCTGAATA AGCCCTCGTA ATATATTTTC ATGAAGAATT TAGGTCCAAA AAAAAGATGG 4801 GCATTAATTC TAGTCATTTA AAAAATTCTA TAGATCAGAG GTTACATGGC CAAGATTGAA 4861 ACTTAGAGGA GTATAGTTAC ATAAAAGAAG GCAAAACGAT GTATAAATGA AAGAAATTGA 4921 GATGGTGCAC GATGCACAGT TGAAGTGAAC TTGCGGGGTT TTTCAGTATC TACGATTCAT 4981 AGATCTCTCG AGCTCGAATT CGGATCCCCG GGGCCTCCAT GGCCATATGA CCACCCAAGC 5041 TAGCGTAATC TGGAACATCG TATGGGTAAG CCATACAGAT CTCTTTTTTT GGGTTTGGTG 5101 GGGTATCTTC ATCATCGAAT AGATAGTTAT ATACATCATC CATTGTAGTG GTATTAAACA 5161 TCCCTGTAGT GATTCCAAAC GCGTTATACG CAGTTTGGTC CGTCCAACCA GGTGACAGTG 5221 GTTTTGAATT ATTACCATCA TCAATTTTAC TAGCCGTGAT TTCATTATTC ATGAAGTTAT 5281 CATGAACGTT AGAGGAGGCA ATTGGTTGTG AAAGCGCTTG AGAATTTGTT TGAGTTGTTA 5341 TGAGGTTCGG ACCGTTGCTA CTGTTAGTGA AAGTGAAGGA CAATGAGCTA TCAGCAATAT 5401 TCCCACTTTG ATTAAAATTG GCGGCGGTAC CCAATTCGAC CTTTCTCTTC TTTTTTGGAG 5461 GCTCGGGAAT TAATTCCGCT TTATCCATCT TTGCAAAGCT TGGAGTTGAT TGTATGCTTG 5521 GTATAGCTTG AAATATTGTG CAGAAAAAGA AACAAGGAAG AAAGGGAACG AGAACAATGA 5581 CGAGGAAACA AAAGATTAAT AATTGCAGGT CTATTTATAC TTGATAGCAA AGCGGCAAAC 5641 TTTTTTTATT TCAAATTCAA GTAACTGGAA GGAAGGCCGT ATACCGTTGC TCATTAGAGA 5701 GTAGTGTGCG TGAATGAAGG AAGGAAAAAG TTTCGTGTGT TCGAAGATAC CCCTCATCAG 5761 CTCTGGAACA ACGACATCTG TTGGTGCTGT CTTTGTCGTT AATTTTTTCC TTTAGTGTCT 5821 TCCATCATTT TTTTTGTCAT TGCGGATATG GTGAGACAAC AACGGGGGAG AGAGAAAAGA 5881 AAAAAAAAGA AAAGAAGTTG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA 5941 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA 6001 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA 6061 CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 6121 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 6181 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 6241 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 6301 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 6361 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 6421 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 6481 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 6541 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 6601 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 6661 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 6721 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 6781 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 6841 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 6901 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 6961 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 7021 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 7081 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 7141 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 7201 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 7261 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 7321 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 7381 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 7441 CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 7501 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 7561 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 7621 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 7681 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 7741 CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 7801 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 7861 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 7921 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 7981 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 8041 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 8101 CCCGAAAAGT GCCACCT //
