pBridge
pBridge
pBridge
编号 | 载体名称 |
北京华越洋生物VECT2390 | pBridge |
pBridge载体基本信息
| 载体名称: | pBridge |
|---|---|
| 质粒类型: | 酵母三杂交系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | ADH1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6526bp |
| 5' 测序引物及序列: | Match: 5'd[TCATCGGAAGAGAGTAGT]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | TRP1 |
| 备注: | Used as bait (& bridge) for yeast 3-hybrid screen. |
| 稳定性: | -- |
| 组成型: | 组成型 |
| 病毒/非病毒: | -- |
pBridge载体质粒图谱和多克隆位点信息
pBridge载体序列
LOCUS pBridge 6526 bp DNA SYN 30-Oct-2013 DEFINITION pBridge ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6526 /organism="pBridge" /mol_type="other DNA" promoter 10..406 /label="yADH1_promoter" misc_feature 434..874 /label="Gal4_DBD" misc_feature 838..856 /label="Gal4_N_term_primer" misc_feature complement(976..1000) /label="pHybLex_rev_primer" terminator 921..1112 /label="yADH1_terminator" CDS complement(1161..1835) /label="TRP1" /gene="TRP1" /note="ORF frame 3" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" gene complement(1161..1835) /label="TRP1" /gene="TRP1" misc_feature complement(1964..1986) /label="M13_pUC_rev_primer" promoter complement(2000..2029) /label="lac_promoter" rep_origin complement(3341..3960) /label="pBR322_origin" CDS complement(4115..4975) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4115..4975) /label="Ampicillin" /gene="Ampicillin" promoter complement(5017..5045) /label="AmpR_promoter" misc_feature complement(5204..5226) /label="pGEX_3_primer" rep_origin 5362..6526 /label="2micron2_origin" ORIGIN 1 GCTTGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC 61 ATATCCGCAA TGACAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC 121 AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT 181 TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT 241 CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA 301 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT 361 CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTGAAGCA 421 AGCCTCCTGA AAGATGAAGC TACTGTCTTC TATCGAACAA GCATGCGATA TTTGCCGACT 481 TAAAAAGCTC AAGTGCTCCA AAGAAAAACC GAAGTGCGCC AAGTGTCTGA AGAACAACTG 541 GGAGTGTCGC TACTCTCCCA AAACCAAAAG GTCTCCGCTG ACTAGGGCAC ATCTGACAGA 601 AGTGGAATCA AGGCTAGAAA GACTGGAACA GCTATTTCTA CTGATTTTTC CTCGAGAAGA 661 CCTTGACATG ATTTTGAAAA TGGATTCTTT ACAGGATATA AAAGCATTGT TAACAGGATT 721 ATTTGTACAA GATAATGTGA ATAAAGATGC CGTCACAGAT AGATTGGCTT CAGTGGAGAC 781 TGATATGCCT CTAACATTGA GACAGCATAG AATAAGTGCG ACATCATCAT CGGAAGAGAG 841 TAGTAACAAA GGTCAAAGAC AGTTGACTGT ATCGCCGGAA TTCCCGGGGA TCCGTCGACC 901 TGCAGCCAAG CTAATTCCGG GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG 961 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT 1021 TCTTGTTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGCATGAGG 1081 TCGCTCTTAT TGACCACACC TCTACCGGCA TGCCGGCAAG TGCACAAACA ATACTTAAAT 1141 AAATACTACT CAGTAATAAC CTATTTCTTA GCATTTTTGA CGAAATTTGC TATTTTGTTA 1201 GAGTCTTTTA CACCATTTGT CTCCACACCT CCGCTTACAT CAACACCAAT AACGCCATTT 1261 AATCTAAGCG CATCACCAAC ATTTTCTGGC GTCAGTCCAC CAGCTAACAT AAAATGTAAG 1321 CTTTCGGGGC TCTCTTGCCT TCCAACCCAG TCAGAAATCG AGTTCCAATC CAAAAGTTCA 1381 CCTGTCCCAC CTGCTTCTGA ATCAAACAAG GGAATAAACG AATGAGGTTT CTGTGAAGCT 1441 GCACTGAGTA GTATGTTGCA GTCTTTTGGA AATACGAGTC TTTTAATAAC TGGCAAACCG 1501 AGGAACTCTT GGTATTCTTG CCACGACTCA TCTCCATGCA GTTGGACGAT ATCAATGCCG 1561 TAATCATTGA CCAGAGCCAA AACATCCTCC TTAGGTTGAT TACGAAACAC GCCAACCAAG 1621 TATTTCGGAG TGCCTGAACT ATTTTTATAT GCTTTTACAA GACTTGAAAT TTTCCTTGCA 1681 ATAACCGGGT CAATTGTTCT CTTTCTATTG GGCACACATA TAATACCCAG CAAGTCAGCA 1741 TCGGAATCTA GAGCACATTC TGCGGCCTCT GTGCTCTGCA AGCCGCAAAC TTTCACCAAT 1801 GGACCAGAAC TACCTGTGAA ATTAATAACA GACATACTCC AAGCTGCCTT TGTGTGCTTA 1861 ATCACGTATA CTCACGTGCT CAATAGTCAC CAATGCCCTC CCTCTTGGCC CTCTCCTTTT 1921 CTTTTTTCGA CCGAATTAAT TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA 1981 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 2041 CTAATGAGTG AGGTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 2101 AAACCTGTCG TGCCAGGAAG ATCCGAGGCC TAGCTTCTAA TTCTTCCAAC ATACAATGGG 2161 AGTTTGGCCG AGTGGTTTAA GGCGTCAGAT TTAGGTGGAT TTAACCTCTA AAATCTCTGA 2221 TATCTTCGGA TGCAAGGGTT CGAATCCCTT AGCTCTCATT ATTTTTTGCT TTTTCTCTTG 2281 AGGTCACATG ATCGCAAAAT GGCAAATGGC ACGTGAAGCT GTCGATATTG GGGAACTGTG 2341 GTGGTTGGCA AATGACTAAT TAAGTTAGTC AAGGCGCCAT CCTCATGAAA ACTGTGTAAC 2401 ATAATAACCG AAGTGTCGAA AAGGTGGCAC CTTGTCCAAT TGAACACGCT CGATGAAAAA 2461 AATAAGATAT ATATAAGGTT AAGTAAAGCG TCTGTTAGAA AGGAAGTTTT TCCTTTTTCT 2521 TGCTCTCTTG TCTTTTCATC TACTATTTCC TTCGTGTAAT ACAGGGTCGT CAGATACATA 2581 GATACAATTC TATTACCCCC ATCCATACAA TGGGCCATAT GGCTTCTAGC TATCCTTATG 2641 ACGTGCCTGA CTATGCCAGC CTGGGAGGAC CTTCTAGTCC TAAGAAGAAG AGAAAGGTGG 2701 CGGCCGCATT AGCCCGAAGA TCTTCGGGCT GATCTCCCAT GTCTCTACTG GTGGTGGTGC 2761 TTCTTTGGAA TTATTGGAAG GTAAGGAATT GCCAGGTGTT GCTTTCTTAT CCGAAAAGAA 2821 ATAAATTGAA TTGAATTGAA ATCGATAGAT CAATTTTTTT CTTTTCTCTT TCCCCATCCT 2881 TTACGCTAAA ATAATAGTTT ATTTTATTTT TTGAATATTT TTTATTTATA TACGTATATA 2941 TAGACTATTA TTTATCTTTT AATGATTATT AAGATTTTTA TTAAAAAAAA ATTCGCTCCT 3001 CTTTTAATGC CTTTATGCAG TTTTTTTTTC CCATTCGATA TTTCTATGTT CGGGTTCAGC 3061 GTATTTTAAG TTTAATAACT CGAAAATTCT GCGTTCGTTA AAGCTAGGCC TCGGATCTTC 3121 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGTTT GCGTATTGGG CGCTCTTCCG 3181 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 3241 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 3301 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 3361 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 3421 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 3481 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 3541 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 3601 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 3661 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 3721 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 3781 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 3841 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 3901 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 3961 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 4021 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 4081 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 4141 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 4201 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 4261 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 4321 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 4381 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 4441 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 4501 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 4561 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 4621 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 4681 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 4741 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 4801 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 4861 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 4921 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 4981 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 5041 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 5101 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA 5161 CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC 5221 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG 5281 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA 5341 TTGTACTGAG AGTGCACCAT AACGCATTTA AGCATAAACA CGCACTATGC CGTTCTTCTC 5401 ATGTATATAT ATATACAGGC AACACGCAGA TATAGGTGCG ACGTGAACAG TGAGCTGTAT 5461 GTGCGCAGCT CGCGTTGCAT TTTCGGAAGC GCTCGTTTTC GGAAACGCTT TGAAGTTCCT 5521 ATTCCGAAGT TCCTATTCTC TAGCTAGAAA GTATAGGAAC TTCAGAGCGC TTTTGAAAAC 5581 CAAAAGCGCT CTGAAGACGC ACTTTCAAAA AACCAAAAAC GCACCGGACT GTAACGAGCT 5641 ACTAAAATAT TGCGAATACC GCTTCCACAA ACATTGCTCA AAAGTATCTC TTTGCTATAT 5701 ATCTCTGTGC TATATCCCTA TATAACCTAC CCATCCACCT TTCGCTCCTT GAACTTGCAT 5761 CTAAACTCGA CCTCTACATT TTTTATGTTT ATCTCTAGTA TTACTCTTTA GACAAAAAAA 5821 TTGTAGTAAG AACTATTCAT AGAGTGAATC GAAAACAATA CGAAAATGTA AACATTTCCT 5881 ATACGTAGTA TATAGAGACA AAATAGAAGA AACCGTTCAT AATTTTCTGA CCAATGAAGA 5941 ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA ATCCACATCG GTATAGAATA 6001 TAATCGGGGA TGCCTTTATC TTGAAAAAAT GCACCCGCAG CTTCGCTAGT AATCAGTAAA 6061 CGCGGGAAGT GGAGTCAGGC TTTTTTTATG GAAGAGAAAA TAGACACCAA AGTAGCCTTC 6121 TTCTAACCTT AACGGACCTA CAGTGCAAAA AGTTATCAAG AGACTGCATT ATAGAGCGCA 6181 CAAAGGAGAA AAAAAGTAAT CTAAGATGCT TTGTTAGAAA AATAGCGCTC TCGGGATGCA 6241 TTTTTGTAGA ACAAAAAAGA AGTATAGATT CTTTGTTGGT AAAATAGCGC TCTCGCGTTG 6301 CATTTCTGTT CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG 6361 TTGCATTTTT GTTTTACAAA AATGAAGCAC AGATTCTTCG TTGGTAAAAT AGCGCTTTCG 6421 CGTTGCATTT CTGTTCTGTA AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC 6481 TCGCGTTGCA TTTTTGTTCT ACAAAATGAA GCACAGATGC TTCGTT //
pBridge其他酵母表达载体：
