pRS414

ꄴ上一个： pRS405
ꄲ下一个： pSEP2
pRS414
编号	载体名称
北京华越洋生物VECT2350	pRS414
pRS414载体基本信息

载体名称:	pRS414
质粒类型:	酿酒酵母载体；酵母着丝粒质粒；穿梭载体
克隆方法:	--
高拷贝/低拷贝:	多拷贝
启动子:	--
载体大小:	4788 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	Ampicillin
筛选标记:	TRP1
克隆宿主:	--
备注:	着丝粒: CEN6；含a beta-gal报告基因；宿主菌株YPH499，YPH500，YPH501
稳定性:	--
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒
pRS414载体质粒图谱和多克隆位点信息

pRS414载体图谱
pRS414载体序列

LOCUS       pRS414	4788 bp 	DNA	SYN
DEFINITION  pRS414
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="pRS414"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     gene            468..1142
                     /label="TRP1"
                     /gene="TRP1"
     CDS             468..1142
                     /label="ORF frame 3"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     CDS             complement(1479..2057)
                     /label="ORF frame 2"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(1712..1855)
                     /label="lacZ_a"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    1826..1848
                     /label="M13_pUC_fwd_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        1841..1857
                     /label="M13_forward20_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        1867..1885
                     /label="T7_promoter"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    1910..1926
                     /label="pBluescriptKS_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(1961..1977)
                     /label="pBluescriptSK_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(2013..2032)
                     /label="T3_promoter"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(2049..2067)
                     /label="M13_reverse_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(2066..2088)
                     /label="M13_pUC_rev_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            complement(3214..4074)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             complement(3214..4074)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4116..4144)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4206..4724
                     /label="CEN6_ARS4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
  241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
  301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
  361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
  421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
  481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
  541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
  601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
  661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
  721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
  781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
  841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
  901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
  961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
 1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
 1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
 1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
 1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
 1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
 1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
 1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
 1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
 1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
 1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
 1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
 1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
 1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
 1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
 1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
 1921 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg
 1981 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
 2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
 2101 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact
 2161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
 2221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
 2281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
 2341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
 2401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
 2461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
 2521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
 2581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
 2641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
 2701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
 2761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
 2821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
 2881 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
 2941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
 3001 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
 3061 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
 3121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
 3181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
 3241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
 3301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
 3361 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
 3421 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
 3481 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
 3541 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
 3601 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
 3661 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
 3721 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
 3781 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
 3841 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
 3901 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
 3961 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
 4021 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
 4081 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
 4141 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
 4201 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
 4261 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
 4321 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
 4381 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
 4441 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
 4501 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
 4561 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
 4621 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
 4681 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta
 4741 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
pRS414其他酵母表达载体：
pPIC6αA	pYD1	pGAG424	pSos-MAFB
pRS406	p53his	pGADT7-T	pBridge
pRS416	pCL1	pHis2	pACT2-AD
pSEP1	pSos	pGBKT7	pFLD/CAT
pHisSi-1	pGBKT7-53	pFLD	pYES2-EGFP
pMyr	pFLDα	Ycp22lac-EGFP	pPIC9k-His
pGBKT7-Lam	pADH2	SUMO protease	pPICZ A
pDisplay	pYIP5	pGADT7	LA503
pPinkα-HC	pDR195	BFD15	pPIC ZαFC
pPIC3.5K	pYES2	pPIC ZαGB	pYEPlac112
pAUR123	pPIC ZαD	pYEPlac195	pYRP7
pPICZ C	Ycplac33	pYX212	pFA6a-FGP(S65T)-kanMX6
pHIC-PI	pYIP211	pRS41H	pESC-URA
pRS426gal	pYES2-kan	pRS316	pYES6/CT
p416GFD	pPICZα B	pESC-His	pYES2-NTB
pYCP211	pESC-Leu	pYES2-NTA	pMET C
pRS426	pYES2-NTC	pMETαA	pPink-HC
pESC-TRP	pMETαB	pPink-LC	pGAPZ B
pYES3/CT	pMET A	pGAPZα C	pPICZα A
pMETαC	pHIL-S1	pPICZ B	pYC2/CT
pMET B	pPICZα C	pPIC9	pRS405
pHIL-D2	pPIC3.5	pGAPZ A	pRS414
pGAPZα A	pUG66	pAD-GAL4-2.1	pSEP2
pAO815	pDEST32	pYC2/NT B	p53blue
pGAPZ C	pYC2/NT A	pPIC6 C	pRS415
pYC2/NT C	pYES-DEST52	pPIC6αB	pSEP3
pYES2/CT	pPIC6αC	pBD-GAL4-Cam
pPIC6 A	pRS403	pRS413