pRS405
pRS405
pRS405
编号 | 载体名称 |
北京华越洋生物VECT2340 | pRS405 |
pRS405载体基本信息
| 载体名称: | pRS405 |
|---|---|
| 质粒类型: | 酿酒酵母载体；酵母着丝粒质粒；穿梭载体 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 高拷贝/低拷贝: | 单拷贝 |
| 启动子: | -- |
| 载体大小: | 5507 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素Ampicillin |
| 筛选标记: | LEU2 |
| 克隆宿主: | -- |
| 备注: | 含a beta-gal报告基因；宿主菌株YPH499，YPH500，YPH501 |
| 稳定性: | -- |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pRS405载体质粒图谱和多克隆位点信息
pRS405载体序列
pRS405, 5507 bp version 013001 NOTE: The following sequence has been verified for accuracy at the junctions. The remainder of the sequence has been obtained from existing data. 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 51 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 101 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG 151 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATCGA CTACGTCGTA 201 AGGCCGTTTC TGACAGAGTA AAATTCTTGA GGGAACTTTC ACCATTATGG 251 GAAATGCTTC AAGAAGGTAT TGACTTAAAC TCCATCAAAT GGTCAGGTCA 301 TTGAGTGTTT TTTATTTGTT GTATTTTTTT TTTTTTAGAG AAAATCCTCC 351 AATATCAAAT TAGGAATCGT AGTTTCATGA TTTTCTGTTA CACCTAACTT 401 TTTGTGTGGT GCCCTCCTCC TTGTCAATAT TAATGTTAAA GTGCAATTCT 451 TTTTCCTTAT CACGTTGAGC CATTAGTATC AATTTGCTTA CCTGTATTCC 501 TTTACTATCC TCCTTTTTCT CCTTCTTGAT AAATGTATGT AGATTGCGTA 551 TATAGTTTCG TCTACCCTAT GAACATATTC CATTTTGTAA TTTCGTGTCG 601 TTTCTATTAT GAATTTCATT TATAAAGTTT ATGTACAAAT ATCATAAAAA 651 AAGAGAATCT TTTTAAGCAA GGATTTTCTT AACTTCTTCG GCGACAGCAT 701 CACCGACTTC GGTGGTACTG TTGGAACCAC CTAAATCACC AGTTCTGATA 751 CCTGCATCCA AAACCTTTTT AACTGCATCT TCAATGGCCT TACCTTCTTC 801 AGGCAAGTTC AATGACAATT TCAACATCAT TGCAGCAGAC AAGATAGTGG 851 CGATAGGGTC AACCTTATTC TTTGGCAAAT CTGGAGCAGA ACCGTGGCAT 901 GGTTCGTACA AACCAAATGC GGTGTTCTTG TCTGGCAAAG AGGCCAAGGA 951 CGCAGATGGC AACAAACCCA AGGAACCTGG GATAACGGAG GCTTCATCGG 1001 AGATGATATC ACCAAACATG TTGCTGGTGA TTATAATACC ATTTAGGTGG 1051 GTTGGGTTCT TAACTAGGAT CATGGCGGCA GAATCAATCA ATTGATGTTG 1101 AACCTTCAAT GTAGGGAATT CGTTCTTGAT GGTTTCCTCC ACAGTTTTTC 1151 TCCATAATCT TGAAGAGGCC AAAAGATTAG CTTTATCCAA GGACCAAATA 1201 GGCAATGGTG GCTCATGTTG TAGGGCCATG AAAGCGGCCA TTCTTGTGAT 1251 TCTTTGCACT TCTGGAACGG TGTATTGTTC ACTATCCCAA GCGACACCAT 1301 CACCATCGTC TTCCTTTCTC TTACCAAAGT AAATACCTCC CACTAATTCT 1351 CTGACAACAA CGAAGTCAGT ACCTTTAGCA AATTGTGGCT TGATTGGAGA 1401 TAAGTCTAAA AGAGAGTCGG ATGCAAAGTT ACATGGTCTT AAGTTGGCGT 1451 ACAATTGAAG TTCTTTACGG ATTTTTAGTA AACCTTGTTC AGGTCTAACA 1501 CTACCGGTAC CCCATTTAGG ACCAGCCACA GCACCTAACA AAACGGCATC 1551 AACCTTCTTG GAGGCTTCCA GCGCCTCATC TGGAAGTGGG ACACCTGTAG 1601 CATCGATAGC AGCACCACCA ATTAAATGAT TTTCGAAATC GAACTTGACA 1651 TTGGAACGAA CATCAGAAAT AGCTTTAAGA ACCTTAATGG CTTCGGCTGT 1701 GATTTCTTGA CCAACGTGGT CACCTGGCAA AACGACGATC TTCTTAGGGG 1751 CAGACATAGG GGCAGACATT AGAATGGTAT ATCCTTGAAA TATATATATA 1801 TATTGCTGAA ATGTAAAAGG TAAGAAAAGT TAGAAAGTAA GACGATTGCT 1851 AACCACCTAT TGGAAAAAAC AATAGGTCCT TAAATAATAT TGTCAACTTC 1901 AAGTATTGTG ATGCAAGCAT TTAGTCATGA ACGCTTCTCT ATTCTATATG 1951 AAAAGCCGGT TCCGGCCTCT CACCTTTCCT TTTTCTCCCA ATTTTTCAGT 2001 TGAAAAAGGT ATATGCGTCA GGCGACCTCT GAAATTAACA AAAAATTTCC 2051 AGTCATCGAA TTTGATTCTG TGCGATAGCG CCCCTGTGTG TTCTCGTTAT 2101 GTTGAGGAAA AAAATAATGG TTGCTAAGAG ATTCGAACTC TTGCATCTTA 2151 CGATACCTGA GTATTCCCAC AGTTAACTGC GGTCAAGATA TTTCTTGAAT 2201 CAGGCGCCTT AGACCGCTCG GCCAAACAAC CAATTACTTG TTGAGAAATA 2251 GAGTATAATT ATCCTATAAA TATAACGTTT TTGAACACAC ATGAACAAGG 2301 AAGTACAGGA CAATTGATTT TGAAGAGAAT GTGGATTTTG ATGTAATTGT 2351 TGGGATTCCA TTTTTAATAA GGCAATAATA TTAGGTATGT GGATATACTA 2401 GAAGTTCTCC TCGACCGTCG ATATGCGGTG TGAAATACCG CACAGATGCG 2451 TAAGGAGAAA ATACCGCATC AGGAAATTGT AAACGTTAAT ATTTTGTTAA 2501 AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC 2551 GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT 2601 GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT 2651 CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT 2701 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT 2751 AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC 2801 CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT 2851 AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC 2901 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC GCGCCATTCG CCATTCAGGC 2951 TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC 3001 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC 3051 AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT 3101 AATACGACTC ACTATAGGGC GAATTGGGTA CCGGGCCCCC CCTCGAGGTC 3151 GACGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGCCCG GGGGATCCAC 3201 TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT 3251 TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 3301 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA GGAGCCGGAA 3351 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGGTA ACTCACATTA 3401 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 3451 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 3501 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 3551 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 3601 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 3651 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 3701 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 3751 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 3801 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 3851 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 3901 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 3951 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 4001 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 4051 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 4101 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 4151 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 4201 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 4251 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 4301 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 4351 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 4401 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 4451 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 4501 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 4551 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 4601 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG 4651 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 4701 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 4751 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 4801 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 4851 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 4901 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA 4951 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 5001 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 5051 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 5101 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 5151 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 5201 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG 5251 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 5301 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 5351 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 5401 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA 5451 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC 5501 TTTCGTC
pRS405其他酵母表达载体：
