pPink-LC
pPink-LC
pPink-LC
编号 | 载体名称 |
北京华越洋生物VECT2260 | pPink-LC |
pPink-LC载体基本信息
载体名称: | pPink-LC |
---|---|
质粒类型: | 毕赤酵母(Pink酵母)蛋白表达载体 |
表达水平: | 低拷贝 |
启动子: | AOX1 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7732 bp |
5' 测序引物及序列: | 5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´; |
3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC |
载体标签: | / |
载体抗性: | 氨苄 |
筛选标记: | ADE2 |
备注: | 细胞内蛋白表达载体 |
稳定性: | 稳定 Stable |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
pPink-LC载体质粒图谱和多克隆位点信息
pPink-LC载体序列
LOCUS pPink-LC 7732 bp ds-DNA circular SYN 04-二月-2013 DEFINITION Pichia pastoris integrating vector for low-copy expression in the PichiaPink(TM) system. ACCESSION . VERSION . KEYWORDS pPink-LC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI COMMENT Linearize within PpTRP2 for integration. FEATURES Location/Qualifiers source 1..7732 /organism="synthetic DNA construct" /lab_host="Pichia pastoris" /mol_type="other DNA" promoter 2..940 /gene="Pichia pastoris AOX1" /note="AOX1 promoter" /note="inducible promoter, regulated by methanol" /note="color: #ffffff; direction: RIGHT" misc_feature 941..987 /note="MCS" /note="multiple cloning site" /note="color: #99ccff" terminator 1013..1262 /gene="S. cerevisiae CYC1" /note="CYC1 terminator" /note="transcription terminator for CYC1" /note="color: #ffffff" promoter 1282..1363 /gene="Pichia pastoris ADE2" /note="PpADE2 LC promoter" /note="strong 82-bp promoter fragment that requires only low-copy integration for efficient expression" /note="color: #ffffff; direction: RIGHT" CDS 1364..3055 /codon_start=1 /gene="Pichia pastoris ADE2" /product="phosphoribosylaminoimidazole carboxylase from Pichia pastoris" /note="PpADE2" /note="essential for the biosynthesis of purine nucleotides, including adenine" /note="color: #ff7f50" promoter 3282..3431 /gene="Pichia pastoris TRP2" /note="PpTRP2 promoter" /note="promoter for Pichia pastoris TRP2" /note="color: #ffffff; direction: RIGHT" CDS 3432..5048 /codon_start=1 /gene="Pichia pastoris TRP2" /product="anthranilate synthase from Pichia pastoris" /note="PpTRP2" /note="catalyzes the initial step of tryptophan biosynthesis" /note="color: #ff7f50" rep_origin complement(5512..6100) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(6271..7131) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: ???1:?6271?..?7062?/?#ccffcc ???2:?7063?..?7131?/?#ccffcc?/?signal?sequence Cleavage site after base 7062" promoter complement(7132..7236) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaaacg gaattccgga ccggcatgca 961 ggcctggtac cggccggcca tttaaataca ggcccctttt cctttgtcga tatcatgtaa 1021 ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa 1081 ggagttagac aacctgaagt ctaggtccct atttattttt tttaatagtt atgttagtat 1141 taagaacgtt atttatattt caaatttttc ttttttttct gtacaaacgc gtgtacgcat 1201 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt 1261 gcaagctgga tccgcggccg cgacttgact cttcactagc ctatgcaaat aaggttacct 1321 tttccaagaa tcgtagaaac gattaaaaaa cttccaaact ctcatggatt ctcaggtaat 1381 aggtattcta ggaggaggcc agctaggccg aatgattgtt gaggccgcta gcaggctcaa 1441 tatcaagacc gtgattcttg atgatggttt ttcacctgct aagcacatta atgctgcgca 1501 agaccacatc gacggatcat tcaaagatga ggaggctatc gccaagttag ctgccaaatg 1561 tgatgttctc actgtagaga ttgagcatgt caacacagat gctctaaaga gagttcaaga 1621 cagaactgga atcaagatat atcctttacc agagacaatc gaactaatca aggataagta 1681 cttgcaaaag gaacatttga tcaagcacaa catttcggtg acaaagtctc agggtataga 1741 atctaatgaa aaggcgctgc ttttgtttgg agaagagaat ggatttccat atctgttgaa 1801 gtcccggact atggcttatg atggaagagg caattttgta gtggagtcta aagaggacat 1861 cagtaaggca ttagagttct tgaaagatcg tccattgtat gccgagaagt ttgctccttt 1921 tgttaaagaa ttagcggtaa tggttgtgag atcactggaa ggcgaagtat tctcctaccc 1981 aaccgtagaa actgtgcaca aggacaatat ctgtcatatt gtgtatgctc cggccagagt 2041 taatgacacc atccaaaaga aagctcaaat attagctgaa aacactgtga agactttccc 2101 aggcgctgga atcttcggag ttgagatgtt cctattgtct gatggagaac ttcttgtaaa 2161 tgagattgct ccaaggcccc acaattctgg tcactataca atcgatgcat gtgtaacatc 2221 tcagttcgaa gcacatgtaa gagccataac tggtctgcca atgccactag atttcaccaa 2281 actatctact tccaacacca acgctattat gctcaatgtt ttgggtgctg aaaaatctca 2341 cggggaatta gagttttgta gaagagcctt agaaacaccc ggtgcttctg tatatctgta 2401 cggaaagacc acccgattgg ctcgtaagat gggtcatatc aacataatag gatcttccat 2461 gttggaagca gaacaaaagt tagagtacat tctagaagaa tcaacccact taccatccag 2521 tactgtatca gctgacacta aaccgttggt tggagttatc atgggttcag actctgatct 2581 acctgtgatt tcgaaaggtt gcgatatttt aaaacagttt ggtgttccat tcgaagttac 2641 tattgtctct gctcatagaa caccacagag aatgaccaga tatgcctttg aagccgctag 2701 tagaggtatc aaggctatca ttgcaggtgc tggtggtgct gctcatcttc caggaatggt 2761 tgctgccatg actccgttgc cagtcattgg tgttcctgtc aagggctcta cgttggatgg 2821 tgtagactcg ctacactcga ttgtccaaat gcctagaggt gttcctgtgg ctacggttgc 2881 tatcaacaac gccaccaatg ccgctctgtt ggccatcagg attttaggta caattgacca 2941 caaatggcaa aaggaaatgt ccaagtatat gaatgcaatg gagaccgaag tgttggggaa 3001 ggcatccaac ttggaatctg aagggtatga atcctatttg aagaatcgtc tttgaattta 3061 gtattgtttt ttaatagatg tatatataat agtacacgta acttatctat tccattcata 3121 attttatttt aaaggttcgg tagaaatttg tcctccaaaa agttggttag agcctggcag 3181 ttttgatagg cattattata gattgggtaa tatttaccct gcacctggag gaactttgca 3241 aagagcctca tgtgcggcgc gccaggccat aatggccaaa cggtttctca attactatat 3301 actactaacc atttacctgt agcgtatttc ttttccctct tcgcgaaagc tcaagggcat 3361 cttcttgact catgaaaaat atctggattt cttctgacag atcatcaccc ttgagcccaa 3421 ctctctagcc tatgagtgta agtgatagtc atcttgcaac agattatttt ggaacgcaac 3481 taacaaagca gatacaccct tcagcagaat cctttctgga tattgtgaag aatgatcgcc 3541 aaagtcacag tcctgagaca gttcctaatc tttaccccat ttacaagttc atccaatcag 3601 acttcttaac gcctcatctg gcttatatca agcttaccaa cagttcagaa actcccagtc 3661 caagtttctt gcttgaaagt gcgaagaatg gtgacaccgt tgacaggtac acctttatgg 3721 gacattcccc cagaaaaata atcaagactg ggcctttaga gggtgctgaa gttgacccct 3781 tggtgcttct ggaaaaagaa ctgaagggca ccagacaagc gcaacttcct ggtattcctc 3841 gtctaagtgg tggtgccata ggatacatct cgtacgattg tattaagtac tttgaaccaa 3901 aaactgaaag aaaactgaaa gatgttttgc aacttccgga agcagctttg atgttgttcg 3961 acacgatcgt ggcttttgac aatgtttatc aaagattcca ggtaattgga aacgtttctc 4021 tatccgttga tgactcggac gaagctattc ttgagaaata ttataagaca agagaagaag 4081 tggaaaagat cagtaaagtg gtatttgaca ataaaactgt tccctactat gaacagaaag 4141 atattattca aggccaaacg ttcacctcta atattggtca ggaagggtat gaaaaccatg 4201 ttcgcaagct gaaagaacat attctgaaag gagacatctt ccaagctgtt ccctctcaaa 4261 gggtagccag gccgacctca ttgcaccctt tcaacatcta tcgtcatttg agaactgtca 4321 atccttctcc atacatgttc tatattgact atctagactt ccaagttgtt ggtgcttcac 4381 ctgaattact agttaaatcc gacaacaaca acaaaatcat cacacatcct attgctggaa 4441 ctcttcccag aggtaaaact atcgaagagg acgacaatta tgctaagcaa ttgaagtcgt 4501 ctttgaaaga cagggccgag cacgtcatgc tggtagattt ggccagaaat gatattaacc 4561 gtgtgtgtga gcccaccagt accacggttg atcgtttatt gactgtggag agattttctc 4621 atgtgatgca tcttgtgtca gaagtcagtg gaacattgag accaaacaag actcgcttcg 4681 atgctttcag atccattttc ccagcaggaa ccgtctccgg tgctccgaag gtaagagcaa 4741 tgcaactcat aggagaattg gaaggagaaa agagaggtgt ttatgcgggg gccgtaggac 4801 actggtcgta cgatggaaaa tcgatggaca catgtattgc cttaagaaca atggtcgtca 4861 aggacggtgt cgcttacctt caagccggag gtggaattgt ctacgattct gacccctatg 4921 acgagtacat cgaaaccatg aacaaaatga gatccaacaa taacaccatc ttggaggctg 4981 agaaaatctg gaccgatagg ttggccagag acgagaatca aagtgaatcc gaagaaaacg 5041 atcaatgaac ggaggacgta agtaggaatt tatggtttgg ccataatggc ctagcttggc 5101 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 5161 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 5221 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 5281 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 5341 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5401 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5461 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5521 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5701 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 5821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 5881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 5941 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 6061 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 6121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6181 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6241 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 6301 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 6361 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6421 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6481 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6541 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6601 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6661 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6721 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6781 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 6841 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 6901 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 6961 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7021 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7081 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 7141 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 7201 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 7261 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 7321 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 7381 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 7441 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 7501 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 7561 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 7621 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 7681 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tg //
pPink-LC其他酵母表达载体: