pMETαA
pMETαA
pMETαA
编号 | 载体名称 |
北京华越洋生物VECT2240 | pMETαA |
pMETαA载体基本信息
| 载体名称: | pMETαA |
|---|---|
| 质粒类型: | 毕赤酵母蛋白表达载体（methanoloica酵母） |
| 表达水平: | 高拷贝 |
| 诱导方法: | 甲醇诱导 |
| 启动子: | AUG1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8043 bp |
| 5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´; alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
| 3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
| 载体标签: | C-V5， C-His |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 利用甲醇诱导蛋白在methanoloica酵母中表达。 |
| 产品目录号: | K1780-01 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pMETαA载体质粒图谱和多克隆位点信息
pMETαA载体序列
LOCUS pMET\alpha\A 8043 bp DNA circular 1-AUG-2013 DEFINITION pMETα A. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI COMMENT ORIGDB|GenBank COMMENT VNTNAME|pMET alpha A| FEATURES Location/Qualifiers misc_feature 1106..1130 /vntifkey="21" /label=AUG1_fwd_primer misc_feature complement(1662..1683) /vntifkey="21" /label=AUG1_rev_primer CDS 3085..3660 /vntifkey="4" /label=ORF\frame\3 CDS 3666..4799 /vntifkey="4" /label=ORF\frame\2 CDS 4963..5961 /vntifkey="4" /label=ORF\frame\3 gene complement(6774..7634) /gene="Ampicillin" /vntifkey="60" CDS complement(6774..7634) /vntifkey="4" /label=ORF\frame\2 promoter complement(7676..7704) /vntifkey="30" /label=AmpR_promoter sig_peptide 1197..1463 /vntifkey="94" /label=Alpha\Factor\Secretion\Signal BASE COUNT 2360 a 1741 c 1568 g 2374 t ORIGIN 1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag 61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt 121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac 181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca 241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac 301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa 361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc 421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa 481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg 541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc 601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa 661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct 721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct 781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg 841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa 901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat 961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct 1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt 1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac 1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga 1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag 1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact 1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg 1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc 1441 tcgagaaaag agaggctgaa gctgaattca cgtggcccag ccggccgtgg atccacgcgt 1501 cgtcgacccg cggcggccgc cagcttccta ggggtaagcc tatccctaac cctctcctcg 1561 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgaactagt atacaattct 1621 agggctgcct gtttggatat ttttataatt tttgagagtt tgccaactaa tgtttttctc 1681 ttctatgata tttatcatgt agttggatat ctttttttct ttgaaaagtt tttatttagt 1741 tctttctctc ttttttataa tgttttcagt ttctttcctt tatattgaaa atacgatgtt 1801 ctttgtcttt cttttttaaa tcctcaatta taatgtgtac cgtttgatca acttgtaaaa 1861 ttgtttgtag tcctgtaatc ttaattgacg attggttgat agtttacccc agtatattgt 1921 gcggctgaga tattacccga ttcggaagat tgattttttc aacagacata aacccatagg 1981 agatttagta cagcgacata tgctaattgc ctgataatgc tcactaacat tttctccttt 2041 taatctggta tatgcataca ggtatgttga tatggccttg ttgatcaaag ctacgaaagt 2101 gtagactatt tcccaaacag cccccaacca ccacctcaca cgcgcacact gcgaagtgaa 2161 agcgctccct tattttcttc tccgctggtt caaaacaaaa aagaatctaa tctgtagaca 2221 cacgccccgt aaaacccaat cttcatcatc gtaattttca gtgacggacg atcgatcaac 2281 catatagttt gttagtgact gagcttcagt agttgacctc ttgaagaaat aacgtcttga 2341 acaacatact ttatacctac caacattcgc catcaacaac agtgtgacac tgacataact 2401 ttacatccat tcatcgacaa aaaaatgtct ctagttcgga ttcatgctta tgggttagct 2461 atttcgccca atgtgtccat ctgacattac tattttgcat tttaatttaa ttagaacttg 2521 actagcgcac taccagcata tcatctcatt tccgtaaata ccaaatgtat tatatattga 2581 aagcttttga ccaggttatt ataaaagaaa cttcatgctc gaaaaagatc atttcgaaaa 2641 gttgcctagt ttcatgaaat tttaaagcag tttatataaa ttttaccttt tgatgcggaa 2701 ttgacttttt cttgaataat acataacttt tcttaaaaga atcaaagaca gataaaattt 2761 aagagatatt aaatattagt gagaagccga gaattttgta acaccaacat aacactgaca 2821 tctttaacaa cttttaatta tgatacattt cttacgtcat gattgattat tacagctatg 2881 ctgacaaatg actcttgttg catggctacg aaccgggtaa tactaagtga ttgactcttg 2941 ctgacctttt attaagaact aaatggacaa tattatggag catttcatgt ataaattggt 3001 gcgtaaaatc gttggatctc tcttctaagt acatcctact ataacaatca agaaaaacaa 3061 gaaaatcgga caaaacaatc aagtatggat tctagaacag ttggtatatt aggaggggga 3121 caattgggac gtatgattgt tgaggcagca aacaggctca acattaagac ggtaatacta 3181 gatgctgaaa attctcctgc caaacaaata agcaactcca atgaccacgt taatggctcc 3241 ttttccaatc ctcttgatat cgaaaaacta gctgaaaaat gtgatgtgct aacgattgag 3301 attgagcatg ttgatgttcc tacactaaag aatcttcaag taaaacatcc caaattaaaa 3361 atttaccctt ctccagaaac aatcagattg atacaagaca aatatattca aaaagagcat 3421 ttaatcaaaa atggtatagc agttacccaa agtgttcctg tggaacaagc cagtgagacg 3481 tccctattga atgttggaag agatttgggt tttccattcg tcttgaagtc gaggactttg 3541 gcatacgatg gaagaggtaa cttcgttgta aagaataagg aaatgattcc ggaagctttg 3601 gaagtactga aggatcgtcc tttgtcgccg aaaaatgggc accatttact aaagaattag 3661 cagtcatgat tgtgagatct gttaacggtt tagtgttttc ttacccaatt gtagagacta 3721 tccacaagga caatatttgt gacttatgtt atgcgcctgc tagagttccg gactccgttc 3781 aacttaaggc gaagttgttg gcagaaaatg caatcaaatc ttttcccggt tgtggtatat 3841 ttggtgtgga aatgttctat ttagaaacag gggaattgct tattaacgaa attgccccaa 3901 ggcctcacaa ctctggacat tataccattg atgcttgcgt cacttctcaa tttgaagctc 3961 atttgagatc aatattggat ttgccaatgc caaagaattt cacatctttc tccaccatta 4021 caacgaacgc cattatgcta aatgttcttg gagacaaaca tacaaaagat aaagagctag 4081 aaacttgcga aagagcattg gcgactccag gttcctcagt gtacttatat ggaaaagagt 4141 ctagacctaa cagaaaagta ggtcacataa atattattgc ctccagtatg gcggaatgtg 4201 aacaaaggct gaactacatt acaggtagaa ctgatattcc aatcaaaatc tctgtcgctc 4261 aaaagttgga cttggaagca atggtcaaac cattggttgg aatcatcatg ggatcagact 4321 ctgacttgcc ggtaatgtct gccgcatgtg cggttttaaa agattttggc gttccatttg 4381 aagtgacaat agtctctgct catagaactc cacataggat gtcagcatat gctatttccg 4441 caagcaagcg tggaattaaa acaattatcg ctggagctgg tggggctgct cacttgccag 4501 gtatggtggc tgcaatgaca ccacttcctg tcatcggtgt gcccgtaaaa ggttcttgtc 4561 tagatggagt agattcttta cattcaattg tgcaaatgcc tagaggtgtt ccagtagcta 4621 ccgtcgctat taataatagt acgaacgctg cgctgttggc tgtcagactg cttggcgctt 4681 atgattcaag ttatacaacg aaaatggaac agtttttatt aaagcaagaa gaagaagttc 4741 ttgtcaaagc acaaaagtta gaaactgtcg gttacgaagc ttatctagaa aacaagtaat 4801 atataagttt attgatatac ttgtacagca aataattata aaatgatata cctatttttt 4861 aggctttgtt atgattacat caaatgtgga cttcatacat agaaatcaac gcttacaggt 4921 gtcctttttt aagaatttca tacataagat ctactagaat caatgttaaa cctgttatca 4981 ttagaacagc catctccaga aggttctatc caagtgaaaa ctttggtctt caagccaaag 5041 actgctaaaa cacaaacccc agtcccaatt attgttgttg ctgaaacttc tacttctact 5101 ccgggtggtg ttatagccaa agcttcgggc actaaagagc caagattagc aaacgatgaa 5161 ttgaccaacc aattctttgg ttctcctgct aaagagtttt ctattgccag cttatcaaac 5221 gaacacaaag acggtaaagt caagatcatt attgatgctg ggatcaaaga tgatgactcg 5281 actaattacg ttatttccaa cactgttgct aagttagttt taaccggtaa gcaattatta 5341 ggtttcttat ccaactacga accaaccatc gttgatttta ctgctgaagc tgcaaccgct 5401 gccccaactc cagttgttaa gaaagaacct gctgcatctg gtaagcttga tgatgccaaa 5461 ttaattggtg ttactgctga caaggaaaaa gatttctcca actggtactc ccaaattcta 5521 atgaagggtg aaatgttaga ctactacgat gtctctggtt gttacattat gagaccagct 5581 tcttacacaa tttgggaaaa gattcaatct tggttcgatt cgaagattaa agaagatggt 5641 gttactaatg cttatttccc aatgtttgtc tcatccaagg ttttagaaaa ggaaaaggac 5701 cacatcgatg gtttcgctcc tgaagttgct tgggttacca gagctggtaa atctgaatta 5761 gaagaaccaa ttgctattag accaacttct gaaactgtta tgggtaccct gcaggggccg 5821 gccttaatta aggcgcgccc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 6421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 7741 acatttcccc gaaaagtgcc acctgtatgc ggtgtgaaat accgcacaga tgcgtaagga 7801 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 7861 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 7921 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 7981 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcaagggc gatggcccac 8041 tac //
pMETαA其他酵母表达载体：
