SUMO protease
SUMO protease
编号 | 载体名称 |
北京华越洋生物VECT2140 | SUMO protease |
SUMO protease载体基本信息
| 载体名称: | SUMO protease |
|---|---|
| 质粒类型: | 酵母表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | -- |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 6XHis |
| 载体抗性: | 卡纳霉素（Kanamycin） |
| 筛选标记: | -- |
| 备注: | 表达SUMO酶 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
SUMO protease载体简介
SUMO Protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae.
It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence.
The SUMO Protease features:
Highly active cleavage
No non-specific proteolysis (highly specific cleavage*)
Activity from 2°C to 37°C
A six-histidine sequence to facilitate its removal from the digested protein sample
Application: Removal of fusion tags from recombinant proteins.Unit Definition: One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2µg of substrate protein at 30°C in one hour.
Quality Control: SUMO Protease has greater than 85% single-band purity with no non-specific protease contamination. It is functionally tested for the absence of any non-specific protease activity.
SUMO protease其他酵母表达载体：
