pHis2

pHis2

pHis2载体基本信息

pHis2载体质粒图谱和多克隆位点信息

pHis2载体序列

LOCUS       pHIS-2	7207 bp 	DNA	SYN
DEFINITION  pHIS-2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="pHIS-2"
                     /mol_type="other DNA"
     gene            152..811
                     /label="HIS3"
                     /gene="HIS3"
     CDS             152..814
                     /label="ORF frame 2"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVA
                     EQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQ
                     AFKEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMI
                     PHFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGV
                     LM*"
     terminator      815..1449
                     /label="HIS3_3UTR_terminator"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVA
                     EQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQ
                     AFKEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMI
                     PHFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGV
                     LM*"
     CDS             1117..1716
                     /label="ORF frame 1"
                     /translation="MAELSEQVQNLSINDNNENGYVPPHLRGKPRSARNNMSNYNNNN
                     GGYNGGRGGGSFFSNNRRGGYGNGGFFGGNNGGSRSNGRSGGRWIDGKHVPAPRNEKA
                     EIAIFGVPEDPLRRTHRGRHHRRHRCGCWRLYRRHHRWGRSGSPLRAHERLFRRGYGG
                     RPRGRGTVGRHLLACTIPCGGGAQRPQPTTGLLPNAGVA*"
     CDS             1385..2350
                     /label="ORF frame 2"
                     /translation="MANMSQLQETKRPRSPYLVSPRILYAGRIVAGITGATGAVAGAY
                     IADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLG
                     CFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            1460..2350
                     /label="tet (300 - 1190)"
                     /gene="tet (300 - 1190)"
                     /translation="MANMSQLQETKRPRSPYLVSPRILYAGRIVAGITGATGAVAGAY
                     IADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLG
                     CFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    complement(1512..1531)
                     /label="pBRrevBam_primer"
                     /translation="MANMSQLQETKRPRSPYLVSPRILYAGRIVAGITGATGAVAGAY
                     IADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLG
                     CFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(1697..2155)
                     /label="ORF frame 1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     gene            2858..3532
                     /label="TRP1"
                     /gene="TRP1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     CDS             2858..3532
                     /label="ORF frame 2"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     promoter        complement(3613..3632)
                     /label="T3_promoter"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     promoter        complement(3649..3667)
                     /label="M13_reverse_primer"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     misc_feature    complement(3666..3688)
                     /label="M13_pUC_rev_primer"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     promoter        complement(3702..3731)
                     /label="lac_promoter"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     CDS             complement(4814..5608)
                     /label="ORF frame 1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(4817..5596)
                     /label="NeoR/KanR (variant)"
                     /gene="NeoR/KanR (variant)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     promoter        complement(5650..5678)
                     /label="AmpR_promoter"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    complement(5741..6257)
                     /label="CEN6_ARS4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    complement(6356..6378)
                     /label="pGEX_3_primer"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     rep_origin      complement(6696..7002)
                     /label="f1_origin"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(6802..59)
                     /label="ORF frame 3"
                     /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV
                     TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT
                     ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(7035..7183)
                     /label="lacZ_a"
                     /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV
                     TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT
                     ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    7149..7171
                     /label="M13_pUC_fwd_primer"
                     /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV
                     TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT
                     ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        7164..7180
                     /label="M13_forward20_primer"
                     /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV
                     TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT
                     ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        7187..7205
                     /label="T7_promoter"
                     /translation="MPGISRPRIDSRTRELPGNSPYSESYYNSLAVVLQRRDWENPGV
                     TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVT
                     ATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
ORIGIN
    1 GAATTCCCGG GGAGCTCACG CGTTCGCGAA TCGATCCGCG GTCTAGAAAT TCCTGGCATT
   61 ATCACATAAT GAATTATACA TTATATAAAG TAATGTGATT TCTTCGAAGA ATATACTAAA
  121 AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC TAGTAAAGCG
  181 TATTACAAAT GAAACCAAGA TTCAGATTGC GATCTCTTTA AAGGGTGGTC CCCTAGCGAT
  241 AGAGCACTCG ATCTTCCCAG AAAAAGAGGC AGAAGCAGTA GCAGAACAGG CCACACAATC
  301 GCAAGTGATT AACGTCCACA CAGGTATAGG GTTTCTGGAC CATATGATAC ATGCTCTGGC
  361 CAAGCATTCC GGCTGGTCGC TAATCGTTGA GTGCATTGGT GACTTACACA TAGACGACCA
  421 TCACACCACT GAAGACTGCG GGATTGCTCT CGGTCAAGCT TTTAAAGAGG CCCTAGGGGC
  481 CGTGCGTGGA GTAAAAAGGT TTGGATCAGG ATTTGCGCCT TTGGATGAGG CACTTTCCAG
  541 AGCGGTGGTA GATCTTTCGA ACAGGCCGTA CGCAGTTGTC GAACTTGGTT TGCAAAGGGA
  601 GAAAGTAGGA GATCTCTCTT GCGAGATGAT CCCGCATTTT CTTGAAAGCT TTGCAGAGGC
  661 TAGCAGAATT ACCCTCCACG TTGATTGTCT GCGAGGCAAG AATGATCATC ACCGTAGTGA
  721 GAGTGCGTTC AAGGCTCTTG CGGTTGCCAT AAGAGAAGCC ACCTCGCCCA ATGGTACCAA
  781 CGATGTTCCC TCCACCAAAG GTGTTCTTAT GTAGTGACAC CGATTATTTA AAGCTGCAGC
  841 ATACGATATA TATACATGTG TATATATGTA TACCTATGAA TGTCAGTAAG TATGTATACG
  901 AACAGTATGA TACTGAAGAT GACAAGGTAA TGCATCATTC TATACGTGTC ATTCTGAACG
  961 AGGCGCGCTT TCCTTTTTTC TTTTTGCTTT TTCTTTTTTT TTCTCTTGAA CTCGAGAAAA
 1021 AAAATATAAA AGAGATGGAG GAACGGGAAA AAGTTAGTTG TGGTGATAGG TGGCAAGTGG
 1081 TATTCCGTAA GAACAACAAG AAAAGCATTT CATATTATGG CTGAACTGAG CGAACAAGTG
 1141 CAAAATTTAA GCATCAACGA CAACAACGAG AATGGTTATG TTCCTCCTCA CTTAAGAGGA
 1201 AAACCAAGAA GTGCCAGAAA TAACATGAGC AACTACAATA ACAACAACGG CGGCTACAAC
 1261 GGTGGCCGTG GCGGTGGCAG CTTCTTTAGC AACAACCGTC GTGGTGGTTA CGGCAACGGT
 1321 GGTTTCTTCG GTGGAAACAA CGGTGGCAGC AGATCTAACG GCCGTTCTGG TGGTAGATGG
 1381 ATCGATGGCA AACATGTCCC AGCTCCAAGA AACGAAAAGG CCGAGATCGC CATATTTGGT
 1441 GTCCCCGAGG ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC
 1501 GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG
 1561 GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT
 1621 GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT
 1681 ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAC CGATGCCCTT
 1741 GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC
 1801 ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTGGGT
 1861 CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT CGCTTGCGGT
 1921 ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA CCAAACGTTT
 1981 CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCGAC GCGCTGGGCT ACGTCTTGCT
 2041 GGCGTTCGCG ACGCGAGGCT GGATGGCCTT CCCCATTATG ATTCTTCTCG CTTCCGGCGG
 2101 CATCGGGATG CCCGCGTTGC AGGCCATGCT GTCCAGGCAG GTAGATGACG ACCATCAGGG
 2161 ACAGCTTCAA GGATCGCTCG CGGCTCTTAC CAGCCTAACT TCGATCATTG GACCGCTGAT
 2221 CGTCACGGCG ATTTATGCCG CCTCGGCGAG CACATGGAAC GGGTTGGCAT GGATTGTAGG
 2281 CGCCGCCCTA TACCTTGTCT GCCTCCCCGC GTTGCGTCGC GGTGCATGGA GCCGGGCCAC
 2341 CTCGACCTGA ATGGAAGCCG GCGGCACCTC GCTAACGGAT TCACCACTCC AAGAATTGGA
 2401 GCCAATCAAT TCTTGCGGAG AACTGTGAAT GCGCAAACCA ACCCTTGGCA GAACATATCC
 2461 ATCGCGTCCG CCATCTCCAG CAGCCGCACG CGGCGCATCG GGGGGGGGGT TTCAATTCAA
 2521 TTCATCATTT TTTTTTTATT CTTTTTTTTG ATTTCGGTTT CTTTGAAATT TTTTTAACGA
 2581 CATTACTATA TATATAATAT AGGAAGCATT TAATAGAACA GCATCGTAAT ATATGTGTAC
 2641 TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAGATATT CTTTATTGAA AAATAGCTTG
 2701 TCACCTTACG TACAATCTTG ATCCGGAGCT TTTCTTTTTT TGCCGATTAA GAATTAATTC
 2761 GGTCGAAAAA AGAAAAGGAG AGGGCCAAGA GGGAGGGCAT TGGTGACTAT TGAGCACGTG
 2821 AGTATACGTG ATTAAGCACA CAAAGGCAGC TTGGAGTATG TCTGTTATTA ATTTCACAGG
 2881 TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC
 2941 TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT
 3001 TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG
 3061 CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT
 3121 GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA
 3181 CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT
 3241 ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC
 3301 AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA
 3361 GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA
 3421 TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG
 3481 TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC
 3541 TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCCT AGAGTCGACC TGCAGGCATG
 3601 CAAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTTCGAGCTT GGCGTAATCA TGGTCATAGC
 3661 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA
 3721 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT
 3781 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC
 3841 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
 3901 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
 3961 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
 4021 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
 4081 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
 4141 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
 4201 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
 4261 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
 4321 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
 4381 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
 4441 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG
 4501 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
 4561 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
 4621 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
 4681 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA
 4741 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
 4801 CTTGGTCTGA CAGTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT
 4861 CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT
 4921 CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC
 4981 CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT
 5041 CGTCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA
 5101 GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG
 5161 CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG
 5221 TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG
 5281 CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT
 5341 CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA
 5401 GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT
 5461 TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC
 5521 CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC
 5581 CTGCGTGCAA TCCATCTTGT TCAATCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
 5641 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
 5701 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGC GGACGGATCG CTTGCCTGTA
 5761 ACTTACACGC GCCTCGTATC TTTTAATGAT GGAATAATTT GGGAATTTAC TCTGTGTTTA
 5821 TTTATTTTTA TGTTTTGTAT TTGGATTTTA GAAAGTAAAT AAAGAGTAGA AGAGTTACGG
 5881 AATGAAGAAA AAAAAATAAA CAAAGGTTTA AAAAATTTCA ACAAAAAGCG TACTTTACAT
 5941 ATATATTTAT TAGACAAGAA AAGCAGATTA AATAGATATA CATTCGATTA ACGATAAGTA
 6001 AAATGTAAAA TCACAGGATT TTCGTGTGTG GTCTTCTACA CAGACAAGAT GAAACAATTC
 6061 GGCATTAATA CCTGAGAGCA GGAAGAGCAA GATAAAAGGN AGTATTTGTT GGCGATCCCC
 6121 CTAGAGTCTT TTACATCTTC GGAAAACAAA AACTATTTTT TCTTTAATTT CTTTTTTTAC
 6181 TTTCTATTTT TAATTTATAT ATTTATATTA AAAAATTTAA ATTATAATTA TTTTTATAGC
 6241 ACGTGATGAA AAGGACCGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA
 6301 ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT
 6361 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC
 6421 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
 6481 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG
 6541 TAAGGAGAAA ATACCGCATC AGGAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT
 6601 AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA
 6661 TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC
 6721 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG
 6781 CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT
 6841 AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT
 6901 GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC
 6961 GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC
 7021 GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC
 7081 GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC
 7141 AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT
 7201 ATAGGGC

// 

pHis2其他酵母表达载体：