pRS415
pRS415
pRS415
编号 | 载体名称 |
北京华越洋生物VECT2070 | pRS415 |
pRS415载体基本信息
载体名称: | pRS415 |
---|---|
质粒类型: | 酿酒酵母载体;酵母着丝粒质粒;穿梭载体 |
克隆方法: | 多克隆位点,限制性内切酶 |
高拷贝/低拷贝: | -- |
启动子: | -- |
载体大小: | 6021 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素Ampicillin |
筛选标记: | LEU2 |
克隆宿主: | -- |
备注: | 含a beta-gal报告基因;宿主菌株YPH499,YPH500,YPH501 |
稳定性: | -- |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pRS415载体质粒图谱和多克隆位点信息
pRS415载体序列
LOCUS pRS415 6021 bp DNA SYN DEFINITION pRS415 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6021 /organism="pRS415" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" gene complement(663..1757) /label="LEU2 (variant)" /gene="LEU2 (variant)" CDS complement(663..1769) /label="ORF frame 2" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" rep_origin complement(2606..2912) /label="f1_origin" /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" CDS complement(2712..3290) /label="ORF frame 2" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" misc_feature complement(2945..3088) /label="lacZ_a" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" misc_feature 3059..3081 /label="M13_pUC_fwd_primer" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" promoter 3074..3090 /label="M13_forward20_primer" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" promoter 3100..3118 /label="T7_promoter" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" misc_feature 3143..3159 /label="pBluescriptKS_primer" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" misc_feature complement(3194..3210) /label="pBluescriptSK_primer" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" promoter complement(3246..3265) /label="T3_promoter" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" promoter complement(3282..3300) /label="M13_reverse_primer" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" misc_feature complement(3299..3321) /label="M13_pUC_rev_primer" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" gene complement(4447..5307) /label="Ampicillin" /gene="Ampicillin" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*" CDS complement(4447..5307) /label="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5349..5377) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 5439..5957 /label="CEN6_ARS4" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 3121 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc 3181 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt 3241 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc 3301 tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa gcataaagtg 3361 taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc 3421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 3481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 3541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 3601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 3661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 3721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 3781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 3841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 3901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 3961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 4021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 4081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 4141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 4201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 4261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 4321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 4381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 4441 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 4501 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 4561 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 4621 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 4681 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 4741 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 4801 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 4861 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 4921 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 4981 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 5041 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 5101 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 5161 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 5221 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 5281 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 5341 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 5401 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat 5461 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa 5521 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga 5581 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt 5641 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt 5701 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta 5761 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta 5821 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa 5881 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac 5941 aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 6001 gtatcacgag gccctttcgt c //
pRS415其他酵母表达载体: