pUG66
pUG66
pUG66
编号 | 载体名称 |
北京华越洋生物VECT2010 | pUG66 |
pUG66载体基本信息
| 载体名称: | pUG66 |
|---|---|
| 质粒类型: | 酵母菌株基因敲除载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3580 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | Bleomycin 博来霉素 |
| 稳定性: | 稳定 |
| 组成型: | 组成型表达 |
| 病毒/非病毒: | 非病毒 |
| 备注: | pUG66载体是大肠杆菌-酵母穿梭载体，在大肠杆菌中抗性是氨苄。pUG66载体质粒含有来自Tn5载体质粒的Ble基因，使得载体质粒在酵母菌中呈现出博来霉素抗性。 pUG66载体在筛选基因Ble两侧含有loxp基因，通过表达cre重组酶，可以利用loxp基因将Ble s筛选基因删除。 pUG66载体可以作为酵母基因敲除的一个质粒PCR模板。代表性的PCR片段的整合插入宿主菌，使得基因敲除突变子能够进行顺利筛选。 |
pUG66载体质粒图谱和多克隆位点信息
载体简介
pUG66载体序列
LOCUS pUG66 3580 bp DNA circular SYN DEFINITION PCR template vector pUG66, complete sequence. ACCESSION AF298794 VERSION AF298794.1 GI:11344912 KEYWORDS . SOURCE PCR template vector pUG66 ORGANISM PCR template vector pUG66 other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI REFERENCE 1 (bases 1 to 3580) AUTHORS Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H. TITLE A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast JOURNAL Nucleic Acids Res. 30 (6), E23 (2002) PUBMED 11884642 REFERENCE 2 (bases 1 to 3580) AUTHORS Gueldener,U., Heinisch,J.H., Voss,D., Koehler,G. and Hegemann,J.H. TITLE Direct Submission JOURNAL Submitted (23-AUG-2000) Institut fuer Mikrobiologie, Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf 40225, Germany FEATURES Location/Qualifiers source 1..3580 /organism="PCR template vector pUG66" /mol_type="other DNA" /db_xref="taxon:142963" /note="derived from Saccharomyces cerevisiae" misc_feature 54..87 /note="loxP site" regulatory 88..484 /regulatory_class="promoter" /note="from TEF" gene 486..866 /gene="ble" CDS 486..866 /gene="ble" /codon_start=1 /transl_table=11 /product="bleomycin resistance protein" /protein_id="AAG34545.1" /db_xref="GI:11344913" regulatory 873..1131 /regulatory_class="terminator" /note="from TEF" misc_feature 1132..1165 /note="loxP site" gene complement(2254..3114) /gene="AmpR" CDS complement(2254..3114) /gene="AmpR" /codon_start=1 /transl_table=11 /product="ampicillin resistance protein" /protein_id="AAG34546.1" /db_xref="GI:11344914" ORIGIN 1 agaacgcggc cgccagctga agcttcgtac gctgcaggtc gacaaccctt aatataactt 61 cgtataatgt atgctatacg aagttattag gtctagagat ctgtttagct tgcctcgtcc 121 ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac 181 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat 241 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc 301 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc 361 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt 421 catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa 481 caaccatggc cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccacggccg 541 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 601 agcgcgggga tctcaagctg gagttcttcg cccaccccgg gctcgatccc ctcgcgagtt 661 ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg 721 tcggcatcca ggaaaccagc agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt 781 ggggaggcac gatggccgct ttggtcgacc cggacgggac gctcctgcgc ctgatacaga 841 acgaattgct tgcaggcatc tcatgatcag tactgacaat aaaaagattc ttgttttcaa 901 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag 961 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca 1021 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg 1081 atactaacgc cgccatccag tgtcgaaaac gagctctcga gaacccttaa tataacttcg 1141 tataatgtat gctatacgaa gttattaggt gatatcagat ccactagtgg cctatgcggc 1201 cgcggatctg ccggtctccc tatagtgagt cgtattaatt tcgataagcc aggttaacct 1261 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 1321 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 1381 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 1441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 1501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 1561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 1621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 1681 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 1741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 1801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 1861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1921 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 1981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 2041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 2101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 2161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 2221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 2281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 2341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 2401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 2461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 2521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 2581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 2641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 2701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 2761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 2821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 2881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 2941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 3001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 3061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 3121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 3181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 3241 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 3301 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 3361 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 3421 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 3481 tgtactgaga gtgcaccata tggacatatt gtcgttagaa cgcggctaca attaatacat 3541 aaccttatgt atcatacaca tacgatttag gtgacactat //
pUG66其他酵母表达载体：
