pCambia1305.2
pCambia1305.2
pCambia1305.2
产品编号 | 载体名称 |
北京华越洋生物VECT0210 | pCambia1305.2 |
pCambia 1305.2载体基本信息
载体名称: | pCambia1305.2, pCambia 1305.2 |
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质粒类型: | 植物表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CAMV35S |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 11922 bp |
5' 测序引物及序列: | 35S promoter: CTATCCTTCGCAAGACCCTTC |
3' 测序引物及序列: | -- |
载体标签: | 带有分泌信号肽的Gus序列。 |
载体抗性: | 卡那 |
筛选标记: | HPTII |
备注: | -- |
产品目录号: | 1305.2 |
稳定性: | 稳定表达 |
组成型: | 非组成型 |
病毒/非病毒: | 非病毒 |
pCambia 1305.2载体质粒图谱和多克隆位点信息
pCambia 1305.2载体序列
LOCUS pCAMBIA1305.2 11922 bp ds-DNA circular SYN DEFINITION Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and secreted GUSPlus(TM) genes. ACCESSION AF354046 VERSION . KEYWORDS pCAMBIA1305.2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI COMMENT The GenBank record was corrected by inserting a G at position 4815. FEATURES Location/Qualifiers source 1..11922 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS 2..82 /codon_start=1 /product="signal sequence from?Oryza sativa glycine-rich cell wall protein" /label="GRP signal sequence" /translation="MATTKHLALAILVLLSIGMTTSARTLL" CDS join(83..91,282..2099) /codon_start=1 /gene=" " /product="beta-glucuronidase" /label="GUSPlus(TM)" /label="codon-optimized Staphylococcus gusA?gene?with a catalase intron to ensure expression in plants but not bacteria" /protein_id=" " intron 92..281 /label="cat1 intron" /label="castor bean catalase intron, modified" CDS 2106..2123 /codon_start=1 /product="6xHis affinity tag" /label="6xHis" /translation="HHHHHH" terminator 2155..2407 /label="NOS terminator" /label="nopaline synthase terminator and poly(A) signal" misc_feature 2429..2453 /label="RB T-DNA repeat" /label="right border repeat from nopaline C58 T-DNA" CDS 3753..4382 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label="pVS1 StaA" CDS 4811..5884 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label="pVS1 RepA" /label=" " /protein_id=" " misc_feature 6488..6628 /label="bom" /label="basis of mobility region from pBR322" rep_origin complement(6814..7402) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7489..8283) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label="KanR" /label="confers resistance to kanamycin" /protein_id=" " misc_feature 8708..8732 /label="LB T-DNA repeat" /label="left border repeat from nopaline C58 T-DNA" polyA_signal 8810..8984 /label="CaMV poly(A) signal" /label="cauliflower mosaic virus polyadenylation signal" CDS complement(9024..10049) /codon_start=1 /product="hygromycin B phosphotransferase" /label="HygR" /label="confers resistance to hygromycin" promoter complement(10117..10794) /label="CaMV 35S promoter (enhanced)" /label="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 11021..11051 /label="lac promoter" /label="promoter for the E. coli lac operon" protein_bind 11059..11075 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /label="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11083..11099 /label="M13 rev" /label="common sequencing primer, one of multiple similar variants" CDS 11095..11328 /codon_start=1 /gene="lacZ (truncated)" /product="LacZ-alpha fragment of?beta-galactosidase" /label="lacZ-alpha" /protein_id=" " /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC" misc_feature 11109..11165 /gene=" " /label="MCS" /label="pUC18/19 multiple cloning site" primer_bind complement(11169..11185) /label="M13 fwd" /label="common sequencing primer, one of multiple similar variants" promoter 11562..11907 /label="CaMV 35S promoter" /label="strong constitutive promoter from cauliflower mosaic virus" ORIGIN 1 catggctact actaagcatt tggctcttgc catccttgtc ctccttagca ttggtatgac 61 caccagtgca agaaccctcc tagatctgag ggtaaatttc tagtttttct ccttcatttt 121 cttggttagg acccttttct ctttttattt ttttgagctt tgatctttct ttaaactgat 181 ctatttttta attgattggt tatggtgtaa atattacata gctttaactg ataatctgat 241 tactttattt cgtgtgtcta tgatgatgat gatagttaca gaaccgacga actagtctgt 301 acccgatcaa caccgagacc cgtggcgtct tcgacctcaa tggcgtctgg aacttcaagc 361 tggactacgg gaaaggactg gaagagaagt ggtacgaaag caagctgacc gacactatta 421 gtatggccgt cccaagcagt tacaatgaca ttggcgtgac caaggaaatc cgcaaccata 481 tcggatatgt ctggtacgaa cgtgagttca cggtgccggc ctatctgaag gatcagcgta 541 tcgtgctccg cttcggctct gcaactcaca aagcaattgt ctatgtcaat ggtgagctgg 601 tcgtggagca caagggcgga ttcctgccat tcgaagcgga aatcaacaac tcgctgcgtg 661 atggcatgaa tcgcgtcacc gtcgccgtgg acaacatcct cgacgatagc accctcccgg 721 tggggctgta cagcgagcgc cacgaagagg gcctcggaaa agtcattcgt aacaagccga 781 acttcgactt cttcaactat gcaggcctgc accgtccggt gaaaatctac acgaccccgt 841 ttacgtacgt cgaggacatc tcggttgtga ccgacttcaa tggcccaacc gggactgtga 901 cctatacggt ggactttcaa ggcaaagccg agaccgtgaa agtgtcggtc gtggatgagg 961 aaggcaaagt ggtcgcaagc accgagggcc tgagcggtaa cgtggagatt ccgaatgtca 1021 tcctctggga accactgaac acgtatctct accagatcaa agtggaactg gtgaacgacg 1081 gactgaccat cgatgtctat gaagagccgt tcggcgtgcg gaccgtggaa gtcaacgacg 1141 gcaagttcct catcaacaac aaaccgttct acttcaaggg ctttggcaaa catgaggaca 1201 ctcctatcaa cggccgtggc tttaacgaag cgagcaatgt gatggatttc aatatcctca 1261 aatggatcgg cgccaacagc ttccggaccg cacactatcc gtactctgaa gagttgatgc 1321 gtcttgcgga tcgcgagggt ctggtcgtga tcgacgagac tccggcagtt ggcgtgcacc 1381 tcaacttcat ggccaccacg ggactcggcg aaggcagcga gcgcgtcagt acctgggaga 1441 agattcggac gtttgagcac catcaagacg ttctccgtga actggtgtct cgtgacaaga 1501 accatccaag cgtcgtgatg tggagcatcg ccaacgaggc ggcgactgag gaagagggcg 1561 cgtacgagta cttcaagccg ttggtggagc tgaccaagga actcgaccca cagaagcgtc 1621 cggtcacgat cgtgctgttt gtgatggcta ccccggagac ggacaaagtc gccgaactga 1681 ttgacgtcat cgcgctcaat cgctataacg gatggtactt cgatggcggt gatctcgaag 1741 cggccaaagt ccatctccgc caggaatttc acgcgtggaa caagcgttgc ccaggaaagc 1801 cgatcatgat cactgagtac ggcgcagaca ccgttgcggg ctttcacgac attgatccag 1861 tgatgttcac cgaggaatat caagtcgagt actaccaggc gaaccacgtc gtgttcgatg 1921 agtttgagaa cttcgtgggt gagcaagcgt ggaacttcgc ggacttcgcg acctctcagg 1981 gcgtgatgcg cgtccaagga aacaagaagg gcgtgttcac tcgtgaccgc aagccgaagc 2041 tcgccgcgca cgtctttcgc gagcgctgga ccaacattcc agatttcggc tacaagaacg 2101 ctagccatca ccatcaccat cacgtgtgaa ttggtgacca gctcgaattt ccccgatcgt 2161 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt 2221 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg 2281 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata 2341 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta 2401 ctagatcggg aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag 2461 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg 2521 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca 2581 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa 2641 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg 2701 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca 2761 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga 2821 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt 2881 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca 2941 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg 3001 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc 3061 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg 3121 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc 3181 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca 3241 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg 3301 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga 3361 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc 3421 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa 3481 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag 3541 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc 3601 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa 3661 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga 3721 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga 3781 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg 3841 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg 3901 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg 3961 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact 4021 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt 4081 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg 4141 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg 4201 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc 4261 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg 4321 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt 4381 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc 4441 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa 4501 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg 4561 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat 4621 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc 4681 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc 4741 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg 4801 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg 4861 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc 4921 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg 4981 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc 5041 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga 5101 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga 5161 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga 5221 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga 5281 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt 5341 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt 5401 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac 5461 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg 5521 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa 5581 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg 5641 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa 5701 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg 5761 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc 5821 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc 5881 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg 5941 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa 6001 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga 6061 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct 6121 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa 6181 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc 6241 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca 6301 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt 6361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 6421 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 6481 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 6541 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 6601 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 6661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7441 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc 7501 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa 7561 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc 7621 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt 7681 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc 7741 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc 7801 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc 7861 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa 7921 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata 7981 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg 8041 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc 8101 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata 8161 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc 8221 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc 8281 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat 8341 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca 8401 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg 8461 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc 8521 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg 8581 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg 8641 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc 8701 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca 8761 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt 8821 tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata 8881 caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc 8941 taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc 9001 gacagatccg gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg 9061 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg 9121 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc 9181 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga 9241 ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc 9301 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg 9361 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg 9421 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact 9481 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg 9541 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg 9601 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc 9661 gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca 9721 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg 9781 caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg 9841 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt 9901 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc 9961 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca 10021 gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccgggatc tgcgaaagct 10081 cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa 10141 tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt 10201 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct 10261 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca 10321 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt 10381 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc 10441 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg 10501 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca atccacttgc 10561 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca 10621 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga 10681 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct 10741 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc 10801 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc 10861 accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga 10921 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 10981 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 11041 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 11101 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca 11161 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 11221 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 11281 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc 11341 ttggatcaga ttgtcgtttc ccgccttcag tttagcttca tggagtcaaa gattcaaata 11401 gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga 11461 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc 11521 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg 11581 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag 11641 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc 11701 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc 11761 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc 11821 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa 11881 ggaagttcat ttcatttgga gagaacacgg gggactcttg ac //
pCambia1305.2其他植物表达载体: