pCambia1381Z
pCambia1381Z
pCambia1381Z
产品编号 | 载体名称 |
北京华越洋生物VECT0180 | pCambia1381Z |
pCambia1381Z载体基本信息
载体名称: | pcambia1381Z, pCambia 1381Z植物表达载体 |
---|---|
质粒类型: | 植物表达载体 |
Genbank序列号: | AF234306 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 11226 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | GusA |
载体抗性: | Kanamycin |
筛选标记: | HptII/ hygromycin B |
备注: | 本载体用于感染植物细胞,研究外源基因在植物组织细胞中的作用。 在大肠杆菌中,该载体是卡那抗性,在植物细胞中,可以利用潮霉素B进行筛选。 载体含有报告基因gusA. pCambia1381Z载体也含有LacZ基因,可以直接用于蓝白斑筛选。 |
稳定性: | 稳定 |
组成型: | 非组成型 |
病毒/非病毒: | 非病毒 |
pCambia1381Z载体质粒图谱和多克隆位点信息
pCambia1381Z载体序列
LOCUS pCambia1381Z 11226 bp DNA circular SYN DEFINITION Binary vector pCAMBIA-1381Z, complete sequence. ACCESSION AF234306 VERSION AF234306.1 GI:7638107 KEYWORDS . SOURCE Binary vector pCAMBIA-1381Z ORGANISM Binary vector pCAMBIA-1381Z other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P. TITLE The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994) PUBMED 7919218 REFERENCE 2 (bases 1 to 11226) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE A comprehensive set of modular vectors for advanced manipulations and efficient transformation of plants JOURNAL Unpublished REMARK Full description of constructs REFERENCE 3 (bases 1 to 11226) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE Direct Submission JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box 3200, Canberra, ACT 2601, Australia FEATURES Location/Qualifiers source 1..11226 /organism="Binary vector pCAMBIA-1381Z" /mol_type="other DNA" /db_xref="taxon:118398" CDS join(2..16,207..2054) /codon_start=1 /product="GUSA, hexaHis tagged" /protein_id="AAF65372.1" /db_xref="GI:7638111" /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV" exon 2..16 /note="synthetic first gus exon (modular)" intron 17..206 /note="Castorbean catalase intron; modified" exon 207..2054 /note="second gusA exon, His6" misc_feature 2086..2338 /note="nos (nopaline synthase) 3'UTR (polyA signal)" misc_feature 2376..2401 /note="right border T-DNA repeat" misc_feature complement(3442..4442) /note="STA region from pVS1 plasmid" rep_origin complement(5035..6035) /note="pVS1-REP; replication origin from pVS1" misc_feature complement(6445..6705) /note="bom site from pBR322" rep_origin complement(6845..7125) /note="pBR322 origin of replication" CDS complement(7416..8210) /note="aadA (kanamycin resistance) gene amplified from pIG121Hm" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAF65369.1" /db_xref="GI:7638108" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 8635..8660 /note="left border repeat from C58 T-DNA" misc_feature 8727..8935 /note="CaMV 3'UTR (polyA signal)" CDS complement(8951..9976) /note="hptII (hygromycin resistance) gene" /codon_start=1 /product="hygromycin phosphotransferase" /protein_id="AAF65370.1" /db_xref="GI:7638109" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKK" promoter complement(10012..10792) /note="CaMV35S2; CaMV 35S promoter, duplicated" promoter 10927..10981 /note="PlacZ; lacZ promoter" CDS 11009..>11207 /codon_start=1 /product="LacZ alpha, truncated" /protein_id="AAF65371.1" /db_xref="GI:7638110" /translation="MTMITNSRGSVDLQPSLALAVVLQRRDWENPGVTQLNRLAAHPP FASWRNSEEARTDRPSQQLRSL" misc_feature 11023..11058 /note="pUC8 MCS; polylinker" ORIGIN 1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa 241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg 301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag 361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt 421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg 481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg 541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac 601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga 661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg 721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg 781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca 841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag 901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc 961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg 1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct 1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg 1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct 1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga 1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca 1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact 1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga 1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg 1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct 1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt 1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac 1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata 1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat 1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat 1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag 1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga 1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc 2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg 2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa 2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg 2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg 2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca 2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc 2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa 2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt 2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat 2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg 2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga 2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc 3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt 3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg 3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct 3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc 3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg 3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga 3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt 3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac 3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc 3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta 3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa 3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag 3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc 3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc 3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg 3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg 3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg 4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac 4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg 4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct 4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg 4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg 4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag 4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt 4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac 4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat 4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag 4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg 4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt 4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg 4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc 4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca 4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt 4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc 5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg 5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta 5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac 5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc 5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac 5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt 5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc 5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg 5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc 5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa 5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg 5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta 5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag 5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg 5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg 5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa 6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg 6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt 6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc 6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg 6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg 6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata 7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca 7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac 7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca 7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc 7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt 7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg 7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac 7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa 7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc 7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc 8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt 8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg 8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc 8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact 8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt 8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat 8401 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc 8461 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa 8521 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt 8581 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag 8641 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt 8701 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt 8761 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta 8821 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc 8881 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatccggtc 8941 ggcatctact ctatttcttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg 9001 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac 9061 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg 9121 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca 9181 tatacgcccg gagtcgtggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg 9241 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt 9301 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg 9361 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct 9421 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca 9481 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg 9541 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat 9601 cggccgcagc gatcgcatcc atagcctccg cgaccggttg tagaacagcg ggcagttcgg 9661 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc 9721 tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt 9781 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat 9841 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca 9901 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga 9961 gttcaggctt tttcatatct cattgccccc cgggatctgc gaaagctcga gagagataga 10021 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat 10081 atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga 10141 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc 10201 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag 10261 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt 10321 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt 10381 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt 10441 agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg 10501 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac 10561 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg 10621 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc 10681 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg 10741 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcca 10801 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 10861 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 10921 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 10981 ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattccc ggggatccgt 11041 cgacctgcag ccaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 11101 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 11161 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaagc ctagggagga 11221 gtccac //
pCambia1381Z其他植物表达载体: