pCambia3301
pCambia3301
pCambia3301
产品编号 | 载体名称 |
北京华越洋生物VECT0150 | pCambia3301 |
pCambia3301载体基本信息
载体名称: | pcambia3301, pCambia 3301植物表达载体 |
---|---|
质粒类型: | 植物双元表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CAMV35S |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 11319 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | C-GusA |
载体抗性: | 卡那 |
筛选标记: | -- |
备注: | 本载体用于感染植物细胞,研究外源基因在植物组织细胞中的作用。 在大肠杆菌中,该载体是卡那抗性。 载体含有报告基因gusA. |
稳定性: | 稳定 |
组成型: | 非组成型 |
病毒/非病毒: | 非病毒 |
pCambia3301载体质粒图谱和多克隆位点信息
pCambia3301载体序列
LOCUS pCAMBIA3301 11319 bp DNA circular SYN DEFINITION pCAMBIA3301 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..11319 /organism="pCAMBIA3301" /mol_type="other DNA" CDS 2..16 /label="GUS\First\Exon" /translation="MVDLR" misc_feature 17..206 /label="Catalase\Intron" misc_feature 324..343 /label="gusA176" misc_feature complement(521..540) /label="gusA-354" misc_feature 561..587 /label="gusA350" misc_feature 624..645 /label="gusA478" misc_feature complement(815..834) /label="gusA-648" misc_feature 927..946 /label="gusA779" CDS 207..2024 /label="gusA\Second\Exon" misc_feature complement(1119..1138) /label="gusA-952" misc_feature 1247..1266 /label="gusA1099" misc_feature complement(1423..1442) /label="gusA-1256" misc_feature 1554..1573 /label="gusA1403" misc_feature complement(1556..1579) /label="gusA-1400" misc_feature complement(1721..1740) /label="gusA-1551" misc_feature 1852..1871 /label="gusA1701" misc_feature 2086..2338 /label="NOS\polyA" misc_feature 2376..2401 /label="T-BORDER\(R)" misc_feature complement(3442..4442) /label="pVS1\Sta" rep_origin complement(5035..6035) /label="pVS1-REP" misc_feature complement(6445..6705) /label="pBR322\bom\site" rep_origin complement(6845..7125) /label="pBR322\ori" CDS complement(7419..8210) /label="kanamycin\(R)" misc_feature 8635..8660 /label="T\BORDER\(L)" misc_feature 8801..8808 /label="POLY\A\SITE" CDS complement(8918..9469) /label="bar" misc_feature complement(9482..10289) /label="CAMV35S" misc_feature 10534..10584 /label="MCS" CDS 10510..10744 /label="LAC\Z\ALPHA" promoter 10776..11313 /label="35S\promoter" ORIGIN 1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa 241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg 301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag 361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt 421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg 481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg 541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac 601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga 661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg 721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg 781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca 841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag 901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc 961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg 1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct 1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg 1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct 1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga 1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca 1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact 1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga 1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg 1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct 1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt 1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac 1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata 1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat 1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat 1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag 1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga 1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc 2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg 2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa 2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg 2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg 2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca 2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc 2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa 2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt 2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat 2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg 2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga 2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc 3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt 3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg 3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct 3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc 3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg 3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga 3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt 3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac 3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc 3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta 3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa 3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag 3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc 3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc 3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg 3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg 3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg 4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac 4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg 4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct 4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg 4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg 4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag 4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt 4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac 4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat 4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag 4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg 4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt 4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg 4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc 4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca 4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt 4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc 5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg 5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta 5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac 5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc 5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac 5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt 5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc 5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg 5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc 5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa 5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg 5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta 5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag 5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg 5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg 5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa 6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg 6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt 6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc 6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg 6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg 6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata 7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca 7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac 7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca 7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc 7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt 7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg 7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac 7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa 7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc 7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc 8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt 8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg 8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc 8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact 8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt 8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat 8401 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc 8461 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa 8521 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt 8581 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag 8641 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt 8701 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt 8761 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta 8821 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc 8881 tgggaactac tcacacatta ttatggagaa actcgagtca aatctcggtg acgggcagga 8941 ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt 9001 tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt 9061 gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct 9121 gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt 9181 ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc 9241 aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat 9301 agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac 9361 ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt 9421 ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg gtagactcga 9481 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga 9541 acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg 9601 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt 9661 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct 9721 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct 9781 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat 9841 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata 9901 ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt 9961 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct 10021 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg 10081 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg 10141 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt 10201 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc 10261 atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 10321 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 10381 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 10441 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 10501 attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc 10561 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 10621 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 10681 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg 10741 gatcagattg tcgtttcccg ccttcagttt agcttcatgg agtcaaagat tcaaatagag 10801 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc 10861 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa 10921 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta 10981 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata 11041 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt 11101 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg 11161 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact 11221 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga 11281 agttcatttc atttggagag aacacggggg actcttgac //
pCambia3301其他植物表达载体: