pCambia1301
pCambia1301
pCambia1301
产品编号 | 载体名称 |
北京华越洋生物VECT0080 | pCambia1301 |
pCambia1301载体基本信息
载体名称: | pCambia1301 |
---|---|
质粒类型: | 植物表达载体 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 11849bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | Kanamycin |
筛选标记: | Hygromycin |
备注: | |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
pCambia1301载体质粒图谱和多克隆位点信息
pCambia1301载体序列
LOCUS AF234297 11849 bp DNA circular SYN DEFINITION Binary vector pCAMBIA-1301, complete sequence. ACCESSION AF234297 VERSION AF234297.1 GI:7638068 KEYWORDS . SOURCE Binary vector pCAMBIA-1301 ORGANISM Binary vector pCAMBIA-1301 other sequences; artificial sequences; vectors. REFERENCE 1 (sites) AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P. TITLE The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994) PUBMED 7919218 REFERENCE 2 (bases 1 to 11849) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE A comprehensive set of modular vectors for advanced manipulations and efficient transformation of plants JOURNAL Unpublished REMARK Full description of constructs REFERENCE 3 (bases 1 to 11849) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE Direct Submission JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box 3200, Canberra, ACT 2601, Australia FEATURES Location/Qualifiers source 1..11849 /organism="Binary vector pCAMBIA-1301" /mol_type="other DNA" /db_xref="taxon:118389" CDS join(11844..11849,1..9,200..2047) /codon_start=1 /product="GUSA, hexaHis tagged" /protein_id="AAF65342.1" /db_xref="GI:7638072" /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV" exon 9..11844 /note="synthetic first gus exon (modular)" intron 10..199 /note="Castorbean catalase intron; modified" exon 200..2047 /note="second gusA exon, His6" misc_feature 2079..2331 /note="nos (nopaline synthase) 3'UTR (polyA signal)" misc_feature 2369..2394 /note="right border T-DNA repeat" misc_feature complement(3435..4435) /note="STA region from pVS1 plasmid" rep_origin complement(5028..6028) /note="pVS1-REP; replication origin from pVS1" misc_feature complement(6438..6698) /note="bom site from pBR322" rep_origin complement(6838..7118) /note="pBR322 origin of replication" CDS complement(7409..8203) /note="aadA (kanamycin resistance) gene amplified from pIG121Hm" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAF65340.1" /db_xref="GI:7638070" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 8628..8653 /note="left border repeat from C58 T-DNA" misc_feature 8720..8928 /note="CaMV 3'UTR (polyA signal)" CDS complement(8944..9969) /note="hptII (hygromycin resistance) gene" /codon_start=1 /product="hygromycin phosphotransferase" /protein_id="AAF65341.1" /db_xref="GI:7638071" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKK" promoter complement(10005..10785) /note="CaMV35S2; CaMV 35S promoter, duplicated" promoter 10933..10987 /note="PlacZ; lacZ promoter" CDS 11015..11248 /codon_start=1 /product="LacZ alpha fragment" /protein_id="AAF65339.1" /db_xref="GI:7638069" /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC" misc_feature 11029..11085 /note="pUC18 MCS; polylinker" promoter 11299..11836 /note="CaMV35S; 35S promoter from CaMV" ORIGIN 1 gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac ccttttctct 61 ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat tgattggtta 121 tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg tgtgtctatg 181 atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc caacccgtga 241 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga 301 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt 361 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg 421 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt 481 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta 541 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac 601 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga 661 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca 721 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac 781 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga 841 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag 901 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga 961 actcgaagtc acagccaaaa gccagacaga gtctgatatc tacccgcttc gcgtcggcat 1021 ccggtcagtg gcagtgaagg gccaacagtt cctgattaac cacaaaccgt tctactttac 1081 tggctttggt cgtcatgaag atgcggactt acgtggcaaa ggattcgata acgtgctgat 1141 ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta 1201 cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga 1261 aactgctgct gtcggctttc agctgtcttt aggcattggt ttcgaagcgg gcaacaagcc 1321 gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc 1381 gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc 1441 caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc 1501 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc 1561 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg 1621 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc 1681 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc 1741 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga 1801 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt 1861 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt 1921 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat 1981 gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc accaccacca 2041 cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa 2101 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga 2161 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt 2221 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg 2281 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact 2341 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa 2401 taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc 2461 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt 2521 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc 2581 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt 2641 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc 2701 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac 2761 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc 2821 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt 2881 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc 2941 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc 3001 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt 3061 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag 3121 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac 3181 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg 3241 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc 3301 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa 3361 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag 3421 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa 3481 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg 3541 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt 3601 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac 3661 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac 3721 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt 3781 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct 3841 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg 3901 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa 3961 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc 4021 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc 4081 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct 4141 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg 4201 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga 4261 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag 4321 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag 4381 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg 4441 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc 4501 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag 4561 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga 4621 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt 4681 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc 4741 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt 4801 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat 4861 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg 4921 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc 4981 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt 5041 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag 5101 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg 5161 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg 5221 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg 5281 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca 5341 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct 5401 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg 5461 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg 5521 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac 5581 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg 5641 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg 5701 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc 5761 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc 5821 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca 5881 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa 5941 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg 6001 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg 6061 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc 6121 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa 6181 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg 6241 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac 6301 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 6361 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 6421 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg 6481 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 6541 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 6601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 6661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 6721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 6781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 6841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 6901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 6961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 7021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 7201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 7261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 7321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 7381 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt 7441 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt 7501 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg 7561 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt 7621 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta 7681 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat 7741 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc 7801 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat 7861 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc 7921 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct 7981 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat 8041 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca 8101 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt 8161 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc 8221 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc 8281 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc 8341 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc 8401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc 8461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc 8521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt 8581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat 8641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg 8701 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt 8761 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt 8821 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac 8881 tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg gtcggcatct 8941 actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac 9001 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac 9061 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc 9121 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc 9181 ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg 9241 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc 9301 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac 9361 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca 9421 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt 9481 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta 9541 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc 9601 agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg 9661 caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct 9721 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata 9781 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg 9841 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc 9901 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg 9961 ctttttcata tctcattgcc ccccgggatc tgcgaaagct cgagagagat agatttgtag 10021 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg 10081 aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca 10141 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt 10201 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc 10261 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa 10321 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa 10381 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag 10441 agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac 10501 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc 10561 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc 10621 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag 10681 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt 10741 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc 10801 tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 10861 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 10921 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 10981 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attcgagctc 11041 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt 11101 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 11161 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 11221 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc 11281 ccgccttcag tttagcttca tggagtcaaa gattcaaata gaggacctaa cagaactcgc 11341 cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat 11401 cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt 11461 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct 11521 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg 11581 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga 11641 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc 11701 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 11761 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 11821 gagaacacgg gggactcttg accatggta //
pCambia1301其他植物表达载体: