pCambia1303
pCambia1303
pCambia1303
产品编号 | 载体名称 |
北京华越洋生物VECT0060 | pCambia1303 |
pCambia1303载体基本信息
出品公司: | -- |
---|---|
载体名称: | pcambia1303 |
质粒类型: | 植物双元表达载体 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 12361 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | Kanamycin |
筛选标记: | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
pCambia1303载体质粒图谱和多克隆位点信息
pCambia1303载体序列
LOCUS AF234299 12361 bp DNA circular SYN DEFINITION Binary vector pCAMBIA-1303, complete sequence. ACCESSION AF234299 VERSION AF234299.1 GI:7638078 KEYWORDS . SOURCE Binary vector pCAMBIA-1303 ORGANISM Binary vector pCAMBIA-1303 other sequences; artificial sequences; vectors. REFERENCE 1 (sites) AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P. TITLE The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994) PUBMED 7919218 REFERENCE 2 (bases 1 to 12361) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE A comprehensive set of modular vectors for advanced manipulations and efficient transformation of plants JOURNAL Unpublished REMARK Full description of constructs REFERENCE 3 (bases 1 to 12361) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE Direct Submission JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box 3200, Canberra, ACT 2601, Australia FEATURES Location/Qualifiers source 1..12361 /organism="Binary vector pCAMBIA-1303" /mol_type="other DNA" /db_xref="taxon:118391" CDS 2..2569 /note="gusA (N358Q) - mgfp5* gene fusion; His6" /codon_start=1 /product="GUSA (N358Q)/MGFP5 fusion, hexaHis tagged" /protein_id="AAF65347.1" /db_xref="GI:7638079" /translation="MVDLTSLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESAL QESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKV WVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVS VELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGI RSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTS HYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQA HLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVN VMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIIT EYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGIL RVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASKGEELFTGVVPILV ELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRY PDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE DGNILGHKLEYNYNSHNVYIMADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGD GPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKASHHHHHHV" misc_feature 1088..1090 /note="N358Q glycosylation mutation" misc_feature 2598..2850 /note="nos (nopaline synthase) 3'UTR (polyA signal)" misc_feature 2888..2913 /note="right border T-DNA repeat" misc_feature complement(3954..4954) /note="STA region from pVS1 plasmid" rep_origin complement(5547..6547) /note="pVS1-REP; replication origin from pVS1" misc_feature complement(6957..7217) /note="bom site from pBR322" rep_origin complement(7357..7637) /note="pBR322 origin of replication" CDS complement(7928..8722) /note="aadA (kanamycin resistance) gene amplified from pIG121Hm" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAF65349.1" /db_xref="GI:7638081" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 9147..9172 /note="left border repeat from C58 T-DNA" misc_feature 9239..9447 /note="CaMV 3'UTR (polyA signal)" CDS complement(9463..10488) /note="hptII (hygromycin resistance) gene" /codon_start=1 /product="hygromycin phosphotransferase" /protein_id="AAF65350.1" /db_xref="GI:7638082" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKK" promoter complement(10524..11331) /note="CaMV35S2; CaMV 35S promoter, duplicated" promoter 11452..11506 /note="PlacZ; lacZ promoter" CDS 11534..11767 /codon_start=1 /product="LacZ alpha fragment" /protein_id="AAF65348.1" /db_xref="GI:7638080" /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC" misc_feature 11576..11626 /note="pUC18 MCS; polylinker" promoter 11818..12355 /note="CaMV35S; 35S promoter from CaMV" ORIGIN 1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact 61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg 121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt 181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat 241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg 301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga 361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa 421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa 481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat 541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca 601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga 661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca 721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac 781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc 841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg 901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca 961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga 1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt 1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta 1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct 1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga 1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact 1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac 1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag 1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa 1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc 1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg 1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc 1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg 1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga 1801 aaaaccgcag cagggaggca aacaagctag taaaggagaa gaacttttca ctggagttgt 1861 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga 1921 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa 1981 actacctgtt ccgtggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc 2041 aagataccca gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata 2101 cgtgcaggag aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt 2161 caagtttgag ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga 2221 ggacggaaac atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat 2281 catggccgac aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga 2341 agacggcggc gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatggccc 2401 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa 2461 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg 2521 catggatgaa ctatacaaag ctagccacca ccaccaccac cacgtgtgaa ttggtgacca 2581 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg 2641 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa 2701 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat 2761 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc 2821 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata 2881 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg 2941 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg 3001 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca 3061 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc 3121 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact 3181 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc 3241 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg 3301 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag 3361 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac 3421 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc 3481 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg 3541 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc 3601 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc 3661 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa 3721 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc 3781 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc 3841 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc 3901 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg 3961 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc 4021 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa 4081 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg 4141 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt 4201 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc 4261 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag 4321 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg 4381 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg 4441 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat 4501 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa 4561 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat 4621 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat 4681 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac 4741 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga 4801 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac 4861 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg 4921 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag 4981 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc 5041 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa 5101 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg 5161 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg 5221 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac 5281 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc 5341 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc 5401 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc 5461 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg 5521 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg 5581 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg 5641 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat 5701 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg 5761 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg 5821 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca 5881 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg 5941 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc 6001 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga 6061 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag 6121 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga 6181 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga 6241 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg 6301 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag 6361 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc 6421 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt 6481 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt 6541 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca 6601 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg 6661 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca 6721 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag 6781 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 6841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 6901 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 6961 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 7021 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 7681 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt 7921 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct 7981 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga 8041 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat 8101 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt 8161 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg 8221 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat 8281 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg 8341 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag 8401 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca 8461 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt 8521 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc 8581 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag 8641 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg 8701 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat 8761 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa 8821 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt 8881 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta 8941 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc 9001 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg 9061 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc 9121 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg 9181 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta 9241 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga 9301 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag 9361 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag 9421 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg 9481 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca 9541 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac 9601 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct 9661 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc 9721 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg 9781 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca 9841 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc 9901 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc 9961 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg 10021 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc 10081 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc 10141 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc 10201 ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac 10261 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa 10321 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa 10381 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc 10441 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc 10501 cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc 10561 gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg 10621 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga 10681 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg 10741 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt 10801 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg 10861 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc 10921 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt 10981 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct 11041 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc 11101 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt 11161 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg 11221 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta 11281 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct 11341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 11521 acaggaaaca gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga 11581 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga 11641 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 11701 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 11761 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat 11821 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt 11881 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca 11941 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat 12001 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat 12061 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg 12121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc 12181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt 12241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca 12301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga 12361 c //
pCambia1303其他植物表达载体: