pDEST20
pDEST20
编号 | 载体名称 |
北京华越洋VECT511113 | pDEST20 |
pDEST20 Gateway载体基本信息：
载体名称: | pDEST20 |
质粒类型: | Gateway系统载体；昆虫细胞表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | polyhedrin (P-PH) |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 7066bp |
5' 测序引物及序列: | 5'd[AAATGATAACCATCTCGC]3' |
3' 测序引物及序列: | -- |
载体标签: | GST (N-term) |
载体抗性: | 氨苄青霉素，氯霉素（仅空载体） |
筛选标记: | Gentamycin 2.html' target='_blank'>庆大霉素（Gentamicin） |
克隆菌株: | ccdB阳性筛选 DB3.1 感受态细胞， 阴性筛选用OmniMAX2-T1 感受态细胞 |
细胞系: | Sf9、Sf21、High Five |
备注: | -- |
稳定性: | 瞬表达 或 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 非病毒 |
pDEST20载体质粒图谱和多克隆位点信息：
pDEST20载体简介：
Gateway昆虫细胞表达载体包括：
pDEST8 不带标签
pDEST10 His标签 N末端
pDEST20 GST标签 N末端
pDEST20载体序列：
ORIGIN
1 CTACGTATAC TCCGGAATAT TAATAGATCA TGGAGATAAT TAAAATGATA ACCATCTCGC
61 AAATAAATAA GTATTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT
121 TCCGGATTAT TCATACCGTC CCACCATCGG GCGCGGATCC ATGTCCCCTA TACTAGGTTA
181 TTGGAAAATT AAGGGCCTTG TGCAACCCAC TCGACTTCTT TTGGAATATC TTGAAGAAAA
241 ATATGAAGAG CATTTGTATG AGCGCGATGA AGGTGATAAA TGGCGAAACA AAAAGTTTGA
301 ATTGGGTTTG GAGTTTCCCA ATCTTCCTTA TTATATTGAT GGTGATGTTA AATTAACACA
361 GTCTATGGCC ATCATACGTT ATATAGCTGA CAAGCACAAC ATGTTGGGTG GTTGTCCAAA
421 AGAGCGTGCA GAGATTTCAA TGCTTGAAGG AGCGGTTTTG GATATTAGAT ACGGTGTTTC
481 GAGAATTGCA TATAGTAAAG ACTTTGAAAC TCTCAAAGTT GATTTTCTTA GCAAGCTACC
541 TGAAATGCTG AAAATGTTCG AAGATCGTTT ATGTCATAAA ACATATTTAA ATGGTGATCA
601 TGTAACCCAT CCTGACTTCA TGTTGTATGA CGCTCTTGAT GTTGTTTTAT ACATGGACCC
661 AATGTGCCTG GATGCGTTCC CAAAATTAGT TTGTTTTAAA AAACGTATTG AAGCTATCCC
721 ACAAATTGAT AAGTACTTGA AATCCAGCAA GTATATAGCA TGGCCTTTGC AGGGCTGGCA
781 AGCCACGTTT GGTGGTGGCG ACCATCCTCC AAAATCGGAT CTGGTTCCGC GTCATAATCA
841 AACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT
901 AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC
961 ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT
1021 GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG
1081 AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT
1141 GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG
1201 GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT
1261 CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG
1321 GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT
1381 TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA
1441 GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG
1501 TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT
1561 ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG
1621 GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTCT GTGATGGCTT CCATGTCGGC
1681 AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAATCTAGA
1741 GGATCCGGCT TACTAAAAGC CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT
1801 ATAAGAATAT ATACTGATAT GTATACCCGA AGTATGTCAA AAAGAGGTGT GCTATGAAGC
1861 AGCGTATTAC AGTGACAGTT GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT
1921 CAATATCTCC GGTCTGGTAA GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA
1981 ACGCTGGAAA GCGGAAAATC AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA
2041 CGGCTCTTTT GCTGACGAGA ACAGGGACTG GTGAAATGCA GTTTAAGGTT TACACCTATA
2101 AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG TGATATTATT GACACGCCCG
2161 GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT GTCAGATAAA GTCTCCCGTG
2221 AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG CATGATGACC ACCGATATGG
2281 CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA TCTCAGCCAC CGCGAAAATG
2341 ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA AATGTCAGGC TCCCTTATAC
2401 ACAGCCAGTC TGCAGGTCGA CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA
2461 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT
2521 CGTTCAGCTT TCTTGTACAA AGTGGTTTGA TAGCTTGTCG AGAAGTACTA GAGGATCATA
2581 ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC
2641 CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT
2701 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
2761 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCTGATCA
2821 CTGCTTGAGC CTAGGAGATC CGAACCAGAT AAGTGAAATC TAGTTCCAAA CTATTTTGTC
2881 ATTTTTAATT TTCGTATTAG CTTACGACGC TACACCCAGT TCCCATCTAT TTTGTCACTC
2941 TTCCCTAAAT AATCCTTAAA AACTCCATTT CCACCCCTCC CAGTTCCCAA CTATTTTGTC
3001 CGCCCACAGC GGGGCATTTT TCTTCCTGTT ATGTTTTTAA TCAAACATCC TGCCAACTCC
3061 ATGTGACAAA CCGTCATCTT CGGCTACTTT TTCTCTGTCA CAGAATGAAA ATTTTTCTGT
3121 CATCTCTTCG TTATTAATGT TTGTAATTGA CTGAATATCA ACGCTTATTT GCAGCCTGAA
3181 TGGCGAATGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC
3241 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC
3301 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG
3361 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA
3421 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC
3481 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT
3541 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
3601 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCAGG TGGCACTTTT
3661 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT
3721 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG
3781 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT
3841 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA
3901 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA
3961 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT
4021 ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT
4081 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC
4141 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA
4201 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT
4261 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT
4321 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC
4381 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG
4441 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC
4501 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG
4561 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA
4621 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA
4681 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC
4741 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA
4801 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
4861 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA
4921 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC
4981 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA
5041 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA
5101 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
5161 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT
5221 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC
5281 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC
5341 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC
5401 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC
5461 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT
5521 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG
5581 CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CAGACCAGCC
5641 GCGTAACCTG GCAAAATCGG TTACGGTTGA GTAATAAATG GATGCCCTGC GTAAGCGGGT
5701 GTGGGCGGAC AATAAAGTCT TAAACTGAAC AAAATAGATC TAAACTATGA CAATAAAGTC
5761 TTAAACTAGA CAGAATAGTT GTAAACTGAA ATCAGTCCAG TTATGCTGTG AAAAAGCATA
5821 CTGGACTTTT GTTATGGCTA AAGCAAACTC TTCATTTTCT GAAGTGCAAA TTGCCCGTCG
5881 TATTAAAGAG GGGCGTGGCC AAGGGCATGG TAAAGACTAT ATTCGCGGCG TTGTGACAAT
5941 TTACCGAACA ACTCCGCGGC CGGGAAGCCG ATCTCGGCTT GAACGAATTG TTAGGTGGCG
6001 GTACTTGGGT CGATATCAAA GTGCATCACT TCTTCCCGTA TGCCCAACTT TGTATAGAGA
6061 GCCACTGCGG GATCGTCACC GTAATCTGCT TGCACGTAGA TCACATAAGC ACCAAGCGCG
6121 TTGGCCTCAT GCTTGAGGAG ATTGATGAGC GCGGTGGCAA TGCCCTGCCT CCGGTGCTCG
6181 CCGGAGACTG CGAGATCATA GATATAGATC TCACTACGCG GCTGCTCAAA CCTGGGCAGA
6241 ACGTAAGCCG CGAGAGCGCC AACAACCGCT TCTTGGTCGA AGGCAGCAAG CGCGATGAAT
6301 GTCTTACTAC GGAGCAAGTT CCCGAGGTAA TCGGAGTCCG GCTGATGTTG GGAGTAGGTG
6361 GCTACGTCTC CGAACTCACG ACCGAAAAGA TCAAGAGCAG CCCGCATGGA TTTGACTTGG
6421 TCAGGGCCGA GCCTACATGT GCGAATGATG CCCATACTTG AGCCACCTAA CTTTGTTTTA
6481 GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTGCGTA ACATCGTTGC TGCTCCATAA
6541 CATCAAACAT CGACCCACGG CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG
6601 TACAAAAAAA CAGTCATAAC AAGCCATGAA AACCGCCACT GCGCCGTTAC CACCGCTGCG
6661 TTCGGTCAAG GTTCTGGACC AGTTGCGTGA GCGCATACGC TACTTGCATT ACAGTTTACG
6721 AACCGAACAG GCTTATGTCA ACTGGGTTCG TGCCTTCATC CGTTTCCACG GTGTGCGTCA
6781 CCCGGCAACC TTGGGCAGCA GCGAAGTCGA GGCATTTCTG TCCTGGCTGG CGAACGAGCG
6841 CAAGGTTTCG GTCTCCACGC ATCGTCAGGC ATTGGCGGCC TTGCTGTTCT TCTACGGCAA
6901 GGTGCTGTGC ACGGATCTGC CCTGGCTTCA GGAGATCGGA AGACCTCGGC CGTCGCGGCG
6961 CTTGCCGGTG GTGCTGACCC CGGATGAAGT GGTTCGCATC CTCGGTTTTC TGGAAGGCGA
7021 GCATCGTTTG TTCGCCCAGG ACTCTAGCTA TAGTTCTAGT GGTTGG
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pDEST20其他相关Gateway载体：
