pTRV1
pTRV1
编号 | 载体名称 |
北京华越洋VECT75603 | pTRV1 |
pTRV1载体基本信息：
启动子: | CaMV 35S |
复制子: | ori |
质粒分类: | 植物系列，RNAi载体 |
质粒大小: | 6791bp |
原核抗性: | Kan |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 植物细胞 |
5'测序引物: | 35S:GACGCACAATCCCACTATCC |
3'测序引物: | 根据序列设计引物 |
pTRV1载体图谱：
pTRV1载体简介：
pTRV1 Tobacco rattle virus (TRV) based cDNA clone was placed between the duplicated CaMV 35S promoter and the nopaline synthase terminator in a T-DNA vector
pTRV1载体序列：
ORIGIN
1 ataaaacatt tcaatccttt gaacgcggta gaacgtgcta attggatttt ggtgagaacg
61 cggtagaacg tacttatcac ctacagtttt attttgtttt tctttttggt ttaatctatc
121 cagcttagta ccgagtgggg gaaagtgact ggtgtgccta aaaccttttc tttgatactt
181 tgtaaaaata catacagata caatggcgaa cggtaacttc aagttgtctc aattgctcaa
241 tgtggacgag atgtctgctg agcagaggag tcatttcttt gacttgatgc tgactaaacc
301 tgattgtgag atcgggcaaa tgatgcaaag agttgttgtt gataaagtcg atgacatgat
361 tagagaaaga aagactaaag atccagtgat tgttcatgaa gttctttctc agaaggaaca
421 gaacaagttg atggaaattt atcctgaatt caatatcgtg tttaaagacg acaaaaacat
481 ggttcatggg tttgcggctg ctgagcgaaa actacaagct ttattgcttt tagatagagt
541 tcctgctctg caagaggtgg atgacatcgg tggtcaatgg tcgttttggg taactagagg
601 tgagaaaagg attcattcct gttgtccaaa tctagatatt cgggatgatc agagagaaat
661 ttctcgacag atatttctta ctgctattgg tgatcaagct agaagtggta agagacagat
721 gtcggagaat gagctgtgga tgtatgacca atttcgtgaa aatattgctg cgcctaacgc
781 ggttaggtgc aataatacat atcagggttg tacatgtagg ggtttttctg atggtaagaa
841 gaaaggcgcg cagtatgcga tagctcttca cagcctgtat gacttcaagt tgaaagactt
901 gatggctact atggttgaga agaaaactaa agtggttcat gctgctatgc tttttgctcc
961 tgaaagtatg ttagtggacg aaggtccatt accttctgtt gacggttact acatgaagaa
1021 gaacgggaag atctatttcg gttttgagaa agatccttcc ttttcttaca ttcatgactg
1081 ggaagagtac aagaagtatc tactggggaa gccagtgagt taccaaggga atgtgttcta
1141 cttcgaaccg tggcaggtga gaggagacac aatgcttttt tcgatctaca ggatagctgg
1201 agttccgagg aggtctctat catcgcaaga gtactaccga agaatatata tcagtagatg
1261 ggaaaacatg gttgttgtcc caattttcga tctggtcgaa tcaacgcgag agttggtcaa
1321 gaaagacctg tttgtagaga aacaattcat ggacaagtgt ttggattaca tagctaggtt
1381 atctgaccag cagctgacca taagcaatgt taaatcatac ttgagttcaa ataattgggt
1441 cttattcata aacggggcgg ccgtgaagaa caagcaaagt gtagattctc gagatttaca
1501 gttgttggct caaactttgc tagtgaagga acaagtggcg agacctgtca tgagggagtt
1561 gcgtgaagca attctgactg agacgaaacc tatcacgtca ttgactgatg tgctgggttt
1621 aatatcaaga aaactgtgga agcagtttgc taacaagatc gcagtcggcg gattcgttgg
1681 catggttggt actctaattg gattctatcc aaagaaggta ctaacctggg cgaaggacac
1741 accaaatggt ccagaactat gttacgagaa ctcgcacaaa accaaggtga tagtatttct
1801 gagtgttgtg tatgccattg gaggaatcac gcttatgcgt cgagacatcc gagatggact
1861 ggtgaaaaaa ctatgtgata tgtttgatat caaacggggg gcccatgtct tagacgttga
1921 gaatccgtgc cgctattatg aaatcaacga tttctttagc agtctgtatt cggcatctga
1981 gtccggtgag accgttttac cagatttatc cgaggtaaaa gccaagtctg ataagctatt
2041 gcagcagaag aaagaaatcg ctgacgagtt tctaagtgca aaattctcta actattctgg
2101 cagttcggtg agaacttctc caccatcggt ggtcggttca tctcgaagcg gactgggtct
2161 gttgttggaa gacagtaacg tgctgaccca agctagagtt ggagtttcaa gaaaggtaga
2221 cgatgaggag atcatggagc agtttctgag tggtcttatt gacactgaag cagaaattga
2281 cgaggttgtt ccagcctttt cagctgaatg tgaaagaggg gaaacaagcg gtacaaaggt
2341 gttgtgtaaa cctttaacgc caccaggatt tgagaacgtg ttgccagctg tcaaaccttt
2401 ggtcagcaaa ggaaaaacgg tcaaacgtgt cgattacttc caagtgatgg gaggtgagag
2461 attaccaaaa aggccggttg tcagtggaga cgattctgtg gacgctagaa gagagtttct
2521 gtactactta gatgcggaga gagtcgctca aaatgatgaa attatgtctc tgtatcgtga
2581 ctattcgaga ggagttattc gaactggagg tcagaattac ccgcacggac tgggagtgtg
2641 ggatgtggag atgaagaact ggtgcatacg tccagtggtc actgaacatg cttatgtgtt
2701 ccaaccagac aaacgtatgg atgattggtc gggatactta gaagtggctg tttgggaacg
2761 aggtatgttg gtcaacgact tcgcggtcga aaggatgagt gattatgtca tagtttgcga
2821 tcagacgtat ctttgcaata acaggttgat cttggacaat ttaagtgccc tggatctagg
2881 accagttaac tgttcttttg aattagttga cggtgtacct ggttgtggta agtcgacaat
2941 gattgtcaac tcagctaatc cttgtgtcga tgtggttctc tctactggga gagcagcaac
3001 cgacgacttg atcgagagat tcgcgagcaa aggttttcca tgcaaattga aaaggagagt
3061 gaagacggtt gattcttttt tgatgcattg tgttgatggt tctttaaccg gagacgtgtt
3121 gcatttcgat gaagctctca tggcccatgc tggtatggtg tacttttgcg ctcagatagc
3181 tggtgctaaa cgatgtatct gtcaaggaga tcagaatcaa atttctttca agcctagggt
3241 atctcaagtt gatttgaggt tttctagtct ggtcggaaag tttgacattg ttacagaaaa
3301 aagagaaact tacagaagtc cagcagatgt ggctgccgta ttgaacaagt actatactgg
3361 agatgtcaga acacataacg cgactgctaa ttcgatgacg gtgaggaaga ttgtgtctaa
3421 agaacaggtt tctttgaagc ctggtgctca gtacataact ttccttcagt ctgagaagaa
3481 ggagttggta aatttgttgg cattgaggaa agtggcagct aaagtgagta cagtacacga
3541 gtcgcaagga gagacattca aagatgtagt cctagtcagg acgaaaccta cggatgactc
3601 aatcgctaga ggtcgggagt acttaatcgt ggcgttgtcg cgtcacacac aatcacttgt
3661 gtatgaaact gtgaaagagg acgatgtaag caaagagatc agggaaagtg ccgcgcttac
3721 gaaggcggct ttggcaagat tttttgttac tgagaccgtc ttatgacggt ttcggtctag
3781 gtttgatgtc tttagacatc atgaagggcc ttgcgccgtt ccagattcag gtacgattac
3841 ggacttggag atgtggtacg acgctttgtt tccgggaaat tcgttaagag actcaagcct
3901 agacgggtat ttggtggcaa cgactgattg caatttgcga ttagacaatg ttacgatcaa
3961 aagtggaaac tggaaagaca agtttgctga aaaagaaacg tttctgaaac cggttattcg
4021 tactgctatg cctgacaaaa ggaagactac tcagttggag agtttgttag cattgcagaa
4081 aaggaaccaa gcggcacccg atctacaaga aaatgtgcac gcaacagttc taatcgaaga
4141 gacgatgaag aagttgaaat ctgttgtcta cgatgtggga aaaattcggg ctgatcctat
4201 tgtcaataga gctcaaatgg agagatggtg gagaaatcaa agcacagcgg tacaggctaa
4261 ggtagtagca gatgtgagag agttacatga aatagactat tcgtcttaca tgtatatgat
4321 caaatctgac gtgaaaccta agactgattt aacaccgcaa tttgaatact cagctctaca
4381 gactgttgtg tatcacgaga agttgatcaa ctcgttgttc ggtccaattt tcaaagaaat
4441 taatgaacgc aagttggatg ctatgcaacc acattttgtg ttcaacacga gaatgacatc
4501 gagtgattta aacgatcgag tgaagttctt aaatacggaa gcggcttacg actttgttga
4561 gatagacatg tctaaattcg acaagtcggc aaatcgcttc catttacaac tgcagctgga
4621 gatttacagg ttatttgggc tagatgagtg ggcggccttc ctttgggagg tgtcgcacac
4681 tcaaactact gtgagagata ttcaaaatgg tatgatggcg catatttggt accaacaaaa
4741 gagtggagat gctgatactt ataatgcaaa ttcagataga acactgtgtg cactcttgtc
4801 tgaattacca ttggagaaag cagtcatggt tacatatgga ggagatgact cactgattgc
4861 gtttcctaga ggaacgcagt ttgttgatcc gtgtccaaag ttggctacta agtggaattt
4921 cgagtgcaag atttttaagt acgatgtccc aatgttttgt gggaagttct tgcttaagac
4981 gtcatcgtgt tacgagttcg tgccagatcc ggtaaaagtt ctgacgaagt tggggaaaaa
5041 gagtataaag gatgtgcaac atttagccga gatctacatc tcgctgaatg attccaatag
5101 agctcttggg aactacatgg tggtatccaa actgtccgag tctgtttcag accggtattt
5161 gtacaaaggt gattctgttc atgcgctttg tgcgctatgg aagcatatta agagttttac
5221 agctctgtgt acattattcc gagacgaaaa cgataaggaa ttgaacccgg ctaaggttga
5281 ttggaagaag gcacagagag ctgtgtcaaa cttttacgac tggtaatatg gaagacaagt
5341 cattggtcac cttgaagaag aagactttcg aagtctcaaa attctcaaat ctaggggcca
5401 ttgaattgtt tgtggacggt aggaggaaga gaccgaagta ttttcacaga agaagagaaa
5461 ctgtcctaaa tcatgttggt gggaagaaga gtgaacacaa gttagacgtt tttgaccaaa
5521 gggattacaa aatgattaaa tcttacgcgt ttctaaagat agtaggtgta caactagttg
5581 taacatcaca tctacctgca gatacgcctg ggttcattca aatcgatctg ttggattcga
5641 gacttactga gaaaagaaag agaggaaaga ctattcagag attcaaagct cgagcttgcg
5701 ataactgttc agttgcgcag tacaaggttg aatacagtat ttccacacag gagaacgtac
5761 ttgatgtctg gaaggtgggt tgtatttctg agggcgttcc ggtctgtgac ggtacatacc
5821 ctttcagtat cgaagtgtcg ctaatatggg ttgctactga ttcgactagg cgcctcaatg
5881 tggaagaact gaacagttcg gattacattg aaggcgattt taccgatcaa gaggttttcg
5941 gtgagttcat gtctttgaaa caagtggaga tgaagacgat tgaggcgaag tacgatggtc
6001 cttacagacc agctactact agacctaagt cattattgtc aagtgaagat gttaagagag
6061 cgtctaataa gaaaaactcg tcttaatgca taaagaaatt tattgtcaat atgacgtgtg
6121 tactcaaggg ttgtgtgaat gaagtcactg ttcttggtca cgagacgtgt agtatcggtc
6181 atgctaacaa attgcgaaag caagttgctg acatggttgg tgtcacacgt aggtgtgcgg
6241 aaaataattg tggatggttt gtctgtgttg ttatcaatga ttttactttt gatgtgtata
6301 attgttgtgg ccgtagtcac cttgaaaagt gtcgtaaacg tgttgaaaca agaaatcgag
6361 aaatttggaa acaaattcga cgaaatcaag ctgaaaacat gtctgcgaca gctaaaaagt
6421 ctcataattc gaagacctct aagaagaaat tcaaagagga cagagaattt gggacaccaa
6481 aaagattttt aagagatgat gttcctttcg ggattgatcg tttgtttgct ttttgatttt
6541 attttatatt gttatctgtt tctgtgtata gactgtttga gattggcgct tggccgactc
6601 attgtcttac cataggggaa cggactttgt ttgtgttgtt attttatttg tattttatta
6661 aaattctcaa tgatctgaaa aggcctcgag gctaagagat tattgggggg tgagtaagta
6721 cttttaaagt gatgatggtt acaaaggcaa aaggggtaaa acccctcgcc tacgtaagcg
6781 ttattacgcc c
//
pTRV1载体其他相关的RNAi载体：
