北京华越洋生物
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pmGeos-M-C1

pmGeos-M-C1

pmGeos-M-C1

pmGeos-M-C1

 



编号

载体名称

北京华越洋VECT90248

pmGeos-M-C1

 

pmGeos-M-C1载体基本信息:

启动子:

CMV

复制子:

pUC ori,f1 ori

终止子:

SV40 poly(A) signal

质粒分类:

哺乳细胞,荧光蛋白报告载体

质粒大小:

4692bp

原核抗性:

Kan

克隆菌株:

DH5α

培养条件:

37℃,有氧 LB

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:

CMV-FCGCAAATGGGCGGTAGGCGTG

3'测序引物:

根据序列设计引物

 

pmGeos-M-C1载体质粒图谱和多克隆位点信息:

 

 

pmGeos-M-C1载体简介:

PmGeos-M-C1 is the evolution of the pEGFP-C1 from the plasmid, it is the carrier of the size of mEos3.2, 4692bp this tag is a combination of mEos2 and I102N/H158E/Y189A from me.

 

pmGeos-M-C1载体序列:

ORIGIN

        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG

       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT

      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA

      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC

      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA

      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC

      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG

      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG

      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT

      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA

      601 CCGGTCGCCA CCATGAGTGC GATTAAGCCA GACATGAAGA TCAAACTCCG TATGGAAGGC

      661 AACGTAAACG GGCACCACTT TGTGATCGAC GGAGATGGTA CAGGCAAGCC TTTTGAGGGA

      721 AAACAGAGTA TGGATCTTGA AGTCAAAGAG GGCGGACCTC TGCCTTTTGC CTTTGATATC

      781 CTGACCACTG CATTCATGTA CGGCAACAGG GTATTCGCCA AATATCCAGA CAACATACAA

      841 GACTATTTTA AGCAGTCGTT TCCTAAGGGG TATTCGTGGG AACGAAGCTT GACTTTCGAA

      901 GACGGGGGCA TTTGCATTGC CAGAAACGAC ATAACAATGG AAGGGGACAC TTTCTATAAT

      961 AAAGTTCGAT TTTATGGTAC CAACTTTCCC GCCAATGGTC CAGTTATGCA GAAGAAGACG

     1021 CTGAAATGGG AGCCCTCCAC TGAGAAAATG TATGTGCGTG ATGGAGTGCT GACGGGTGAT

     1081 ATTCATATGG CTTTGTTGCT TGAAGGAAAT GCCCATTACC GATGTGACTT CAGAACTACT

     1141 TACAAAGCTA AGGAGAAGGG TGTCAAGTTA CCAGGCTACC ACTTTGTGGA CCACTGCATT

     1201 GAGATTTTAA GCCATGACAA AGATTACAAC AAGGTTAAGC TGTATGAGCA TGCTGTTGCT

     1261 CATTCTGGAT TGCCTGACAA TGCCAGACGA TCCGGACTCA GATCTCGAGC TCAAGCTTCG

     1321 AATTCTGCAG TCGACGGTAC CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCATA

     1381 ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC

     1441 CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT

     1501 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG

     1561 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT AACGCGTAAA TTGTAAGCGT

     1621 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA

     1681 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT

     1741 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG

     1801 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT

     1861 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC

     1921 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG

     1981 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT

     2041 TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC

     2101 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG

     2161 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTCCTGAG GCGGAAAGAA CCAGCTGTGG

     2221 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA

     2281 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC

     2341 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG

     2401 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT

     2461 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA

     2521 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC

     2581 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG

     2641 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG

     2701 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA

     2761 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC

     2821 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC

     2881 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA

     2941 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA

     3001 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT

     3061 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT

     3121 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT

     3181 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA

     3241 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA

     3301 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG

     3361 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG

     3421 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC

     3481 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG

     3541 TTCTTCGCCC ACCCTAGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA

     3601 ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT

     3661 CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT

     3721 TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA

     3781 GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCCTC AGGTTACTCA

     3841 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC

     3901 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA

     3961 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC

     4021 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA

     4081 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT

     4141 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC

     4201 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG

     4261 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG

     4321 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG

     4381 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC

     4441 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT

     4501 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG

     4561 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC

     4621 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT

     4681 ACCGCCATGC AT

//

 

pmGeos-M-C1其他相关荧光蛋白报告载体

pEF1/V5-HisA

pEGFP-N3

pEF-GFP

pEGFP-C3

pFLAG-CMV2

pEGFP-N2

pCI-NEO

pEGFP-N1

pCEP4

pEGFP-1

pVAX1

pECFP-C1

pcDNA3.1/V5-His B

pECFP-N1

pCDNA6-Myc-HisB

pDsRed2-Nuc

pCDNA3.1-3×HA

pDsRed2-ER

pCDNA3.1-3×HA

Top-Flash

pCDNA3.1(-)/Myc-His B

pSTAT3-TA-luc

pCDNA3.1(-)/Myc-His A

pSilencer2.1-U6 neo

pCDNA3.1(+)/Myc-His B

psiCHECK-2

pCDNA3.1(+)-Zeo

pRL-TK

pCDNA3.1(+)-EGFP

pRL-SV40

pCDNA3.1(+)

pRL-CMV

pCDNA3.1(-)

pp53-TA-luc

pBudCE4.1-hrGFP

pNF-KB-TA-LUC

pCAGGS-HA

pNF-kB-Luc

pVAX1-FLAG

pMIR-Reporter

pSV2-DHFR

pmirGLO

pCEP4/CAT

pGL4.79[hRluc/Neo]

pCAGGS

pGL4.75[hRluc/CMV]

pCAG-GFP

pGL4.73[hRluc /SV40]

pCDNA3.0

pGL4.29[luc2P/CRE/Hygro]

pCDNA3.0-HA

pGL4.13-luci2

pCDNA3.1(+)-3×FLAG

pGL4.13-luci2

pSV-Beta-Gal

pGL4.10[luc2]

SuperCos1

pGL3-Enhancer

pT2/VENUS

pGL3-Control

pAdVAntage

pGL3-Basic

pEYFP-Tubulin

pGL3-3.6kb human IFN-gamma luc

pEGFP-C1

pENTR5'-UbCp

pmGeos-M-N1

pBiFC-VN173

pEYFP-N1

pBiFC-VC155

pEYFP-C1

pBIFC-bFosVC155

pEGFP-C2

pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP)   VC155

pEYFP-Actin

Laconic/pcDNA3.1(-)

pDsRed2-Peroxi

Fop-Flash

pEBFP-C1

pTet-On-Advanced

pmGeos-M-C1

pmCherry-EGFP-LC3b-r

pCerulean-N1

pmCherry-EGFP-LC3b-m

pmKO2-N1

pmCherry-EGFP-LC3B-h

pMyosin-PSmOrange

pEGFP-LC3B-r

pTagBFP-N

pEGFP-LC3B-m

pCAG-DsRed

pEGFP-LC3B-h

pmCherry-C1

pEGFP-LC3B-c

pmCherry-N1

Super PiggyBac Transposase (PB200PA-1)

pEYFP-Golgi

pTol2-Promoter-T2A-EGFP

pEYFP-Mem

pSP64-Tol2-Transposase

pDsRed2-C1

PiggyBac Dual promoter (PB513B-1)

pEGFP-Actin

pBIND-ID

pEBFP-N1

pG5Luc

pDsRed2-N1

pFUSE-hIgG1e1-Fc2

pN3

pEE12.4

pEGFP-EcoRV

pEE6.4