pLentG-KOSM
pLentG-KOSM
编号 | 载体名称 |
北京华越洋VECT231216 | pLentG-KOSM |
pLentG-KOSM载体基本信息：
载体名称: | pLentG-KOSM |
质粒类型: | 慢病毒系统载体 |
高拷贝/低拷贝: | -- |
启动子: | CMV |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 13kb |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | GFP |
载体抗性: | 氨苄青霉素（Ampicillian） |
筛选标记: | -- |
备注: | -- |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
pLentG-KOSM载体质粒图谱和多克隆位点信息：
pLentG-KOSM载体简介：
pLentG-KOSM is a simple lentiviral vector in which defined factors are in-frame fused into a single open
reading frame (ORF) via self-cleaving 2A peptides and are controlled by a CMV promoter. The mouse
transcription factor ORF is followed by IRES-GFP as a reporter for viral transduction. This polycistronic
expression system can efficiently reprogram mouse or human somatic cells into iPS cells; for example, in
infected MEF (GFP+), the reprogramming efficiency is around 1%, which is at least 10-fold higher than
the reprogramming method using a pool of viruses in which each virus expresses a different stem cell
factor gene. Notably, most of the iPS cells generated using the pLentG-KOSM system contain only a
single copy of the viral vector (Ref. 1).
pLentG-KOSM载体序列
pLentG-KOSM其他相关慢病毒载体：
